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    1. Home
    2. Molecular Biology Methods
    Overview
    Isolation & Purification
    Labeling, Detection & More
    Amplification
    Cloning & Modification
    Transfection
    Analysis
    Related Resources
    Other Applications

    Molecular Biology Methods

    Products for Your Workflow

    Molecular biology techniques are used to investigate the structure and function of the cells and tissues of living organisms. These techniques may include DNA and RNA analysis, gene expression, biochemical analysis, and protein production. Find DNA and RNA testing products, including reagents for locating specific DNA sequences, as well as products for transfection, chemical and electroporation transformations, nucleic acid labeling and detection, and more.

    Isolation & Purification


    Well-prepared DNA is critical to your experiments and research, so how you isolate and purify your samples is important. Multiple methods exist for disrupting the cells, removing debris, capturing the DNA, removing contaminants, and eluting the DNA for use in downstream applications. Find products for isolation, extraction, purification, and more to complete this step of your process.

    Top Categories

    RNA Isolation and Purification
    RNA Isolation and Purification
    DNA Extraction and Purification
    DNA Extraction and Purification
    Nucleic Acid Purification Systems
    Nucleic Acid Purification Systems

    Featured Products

    Thermo Scientific™ KingFisher Apex Purification Systems

    Catalog Number 5400910

    Thermo Scientific™ KingFisher Apex Purification Systems DFS Item
    Cytiva Sera-Xtracta Cell-Free DNA Kit

    Catalog Number 09920066

    Cytiva Sera-Xtracta Cell-Free DNA Kit
    MP Biomedicals™ FastRNA™ Pro Soil-Direct Kit

    Catalog Number MP116070050

    MP Biomedicals™ FastRNA™ Pro Soil-Direct Kit

    Resources

    MP Biomedicals Life Sciences Research Flyer

    Filename
    mp-biomedicals-life-science-research-flyer.pdf
    Size
    761 KB
    Format
    application/pdf

    KingFisher Apex Instrument Brochure

    Filename
    thermo-scientific-kingfisher-apex-brochure.pdf
    Size
    3 MB
    Format
    application/pdf

    Invitrogen DNA Purification and Analysis Brochure

    Filename
    invitrogen-purification-analysis-brochure.pdf
    Size
    13 MB
    Format
    application/pdf

    Labeling, Detection & More


    Tags or labels, called probes, are added to nucleic acids to enable detection or purification. Labels may include radioactive phosphates, biotin, fluorophores, and enzymes. Fluorescent in situ hybridization (FISH) is used to identify specific DNA sequences and tests can also be performed for pathogen detection. Find labeling and detection kits, spectrophotometers, fluorometers, and more below for your nucleic acid work.

    Top Categories

    Nucleic Acid Labeling and Detection Kits
    Nucleic Acid Labeling and Detection Kits
    UV-Vis Spectrophotometers
    UV-Vis Spectrophotometers
    Fluorometers and Fluorescence Polarization Systems
    Fluorometers and Fluorescence Polarization Systems

    Featured Products

    Thermo Scientific&trade;&nbsp;NanoDrop&trade; One<sup>C</sup> Microvolume UV-Vis Spectrophotometer

    Catalog Number 13-400-519

    Thermo Scientific™ NanoDrop™ OneC Microvolume UV-Vis Spectrophotometer
    Invitrogen&trade;&nbsp;Qubit&trade; 4 Fluorometer

    Catalog Number Q33226

    Invitrogen™ Qubit™ 4 Fluorometer DFS Item
    Cytiva&nbsp;Amersham&trade; ECL&trade; Direct Nucleic Acid Labeling/Detection System

    Catalog Number 45-000-925

    Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System

    Resources

    Thermo Scientific NanoDrop One Selection Guide

    Filename
    thermo-scientific-nanodrop-one-brochure.pdf
    Size
    3 MB
    Format
    application/pdf

    MP Biomedicals Sample Preparation Brochure

    Filename
    mp-biomedicals-sample-preparation.pdf
    Size
    2 MB
    Format
    application/pdf

    Thermo Scientific Security Software Flyer

    Filename
    thermo-scientific-software-flyer.pdf
    Size
    452 KB
    Format
    application/pdf

    Amplification


    The goal of PCR is the amplification of existing DNA. Ultimately, having multiple copies makes it possible to perform your assays. Choose from a variety of thermal cyclers, polymerases, master mixes, and other PCR systems to support your PCR amplification needs.

    Top Categories

    Thermal Cyclers
    Thermal Cyclers
    DNA Polymerases
    DNA Polymerases
    PCR Supplies
    PCR Supplies

    Featured Products

    Thermo Scientific&trade;&nbsp;NTP Set, Tris buffered

    Catalog Number FERR1481

    Thermo Scientific™ NTP Set, Tris buffered
    Applied Biosystems&trade;&nbsp;ProFlex&trade; PCR System, 3 x 32-well

    Catalog Number 4484073

    Applied Biosystems™ ProFlex™ PCR System, 3 x 32-well DFS Item

    Resources

    Fisher Scientific PCR Workflow Brochure

    Filename
    fisher-scientific-pcr-workflow-brochure.pdf
    Size
    1 MB
    Format
    application/pdf

    PCR Solutions Brochure

    Filename
    pcr-plastics-solutions-brochure-0619.pdf
    Size
    3 MB
    Format
    application/pdf

    Thermo Scientific Molecular Biology Handbook

    Filename
    thermo-scientific-molecular-biology-handbook.pdf
    Size
    24 MB
    Format
    application/pdf

    Cloning & Modification


    Cloning and genetic modification are commonly used to treat genetic defects. The field of genetics can help with DNA manipulation in vivo, altering genes to correct mutations, introduce new genetic information, or remove specific DNA sequences. Shop enzymes and inhibitors, cloning kits, competent cells, and more to help you conduct your cloning and modification research.

    Top Categories

    Enzymes and Inhibitors
    Enzymes and Inhibitors
    PCR Cloning Kits
    PCR Cloning Kits
    Competent Cells
    Competent Cells

    Featured Products

    Invitrogen&trade;&nbsp;One Shot&trade; MAX Efficiency&trade; DH5&alpha;-T1<sup>R</sup> Competent Cells

    Catalog Number 12297016

    Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells DFS Item
    Invitrogen&trade;&nbsp;Lipofectamine&trade; 3000 Transfection Reagent

    Catalog Number L3000001

    Invitrogen™ Lipofectamine™ 3000 Transfection Reagent DFS Item
    Invitrogen&trade;&nbsp;TOPO&trade; TA Cloning&trade; Kit, with pCR&trade;2.1-TOPO&trade;, One Shot Mach1&trade;-T1R Chemically Competent <i>E. coli</i>, and PureLink&trade; Quick Plasmid Miniprep Kit

    Catalog Number K451022

    Invitrogen™ TOPO™ TA Cloning™ Kit, with pCR™2.1-TOPO™, One Shot Mach1™-T1R Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit DFS Item

    Resources

    Thermo Scientific Molecular Cloning Brochure

    Filename
    thermo-scientific-molecular-cloning-brochure.pdf
    Size
    26 MB
    Format
    application/pdf

    CRISPR-Cas9 Gene Editing Products Brochure

    Filename
    biotech-crispr-bro-22-648-0288.pdf
    Size
    6 MB
    Format
    application/pdf

    Transfection


    Mimicking natural viral processes, transfection artificially introduces nucleic acids to cells, producing various chemical, biological, or physical changes. Although the term transfection was originally used to describe the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, the term currently refers to any artificial introduction of foreign nucleic acid into a cell. Search for transfection reagents, electroporation supplies, and more to help effectively conduct your transfection procedures.

    Top Categories

    Transfection Reagents
    Transfection Reagents
    Electroporators
    Electroporators
    Electroporation Cuvets
    Electroporation Cuvets

    Featured Products

    Invitrogen&trade;&nbsp;Lipofectamine&trade; 3000 Transfection Reagent

    Catalog Number L3000001

    Invitrogen™ Lipofectamine™ 3000 Transfection Reagent DFS Item
    BTX&trade;&nbsp;ECM&trade; 830 Square Wave Electroporation System, with Safety Stand

    Catalog Number BTX450661

    BTX™ ECM™ 830 Square Wave Electroporation System, with Safety Stand

    Resources

    Mirus Bio Transfection Experts Brochure

    Filename
    mirus-bio-transfection-experts-brochure.pdf
    Size
    1 MB
    Format
    application/pdf

    Transfection Reagents and Electroporation Brochure

    Filename
    transfection-reagents-electroporation-brochure.pdf
    Size
    1 MB
    Format
    application/pdf

    Analysis


    The yield from your PCR and molecular biology procedures must be further analyzed and evaluated to confirm the outcome of your assays, especially if the material is intended for other downstream applications. Shop electrophoresis equipment, gel stains, imaging and documentation systems, and more to help ensure the success of your molecular biology assays.

    Top Categories

    Gel Electrophoresis Equipment and Supplies
    Gel Electrophoresis Equipment and Supplies
    Electrophoresis Gel Stains
    Electrophoresis Gel Stains
    Gel Imaging
    Gel Imaging

    Featured Products

    Invitrogen&trade;&nbsp;E-Gel&trade; Power Snap Electrophoresis System Starter Kit, EX 2%

    Catalog Number G8342ST

    Invitrogen™ E-Gel™ Power Snap Electrophoresis System Starter Kit, EX 2% DFS Item
    Syngene&trade;&nbsp;G:BOX - F3 with Feedback Lens

    Catalog Number 01257160

    Syngene™ G:BOX - F3 with Feedback Lens
    Invitrogen&trade;&nbsp;SYBR&trade; Safe DNA Gel Stain

    Catalog Number S33102

    Invitrogen™ SYBR™ Safe DNA Gel Stain DFS Item

    Resources

    Fisherbrand Real-Time Electrophoresis System Flyer

    Filename
    fisherbrand-electrophoresis-system-flyer.pdf
    Size
    1 MB
    Format
    application/pdf

    Fisherbrand Electrophoresis Equipment Brochure

    Filename
    electrophoresis-equipment-brochure.pdf
    Size
    1 MB
    Format
    application/pdf

    Related Resources


    Proofreading Enzyme May Improve Genetic Sequencing

    Read About Genetic Sequencing
    Proofreading Enzyme May Improve Genetic Sequencing

    Securing Genomic Samples Against Contamination

    Read About Protecting Your Samples
    Securing Genomic Samples Against Contamination

    The Promise of Oligos in Medicine

    Read About Oligonucleotides
    The Promise of Oligos in Medicine

    Power Your Protein Research From Collection to Analysis

    Shop Protein Research Products
    Power Your Protein Research From Collection to Analysis
    Viewing 1-3 of 4

    Other Applications


    Cell Analysis Methods

    Shop Cell Analysis Products
    Cell Analysis Methods

    PCR and qPCR

    Shop PCR and qPCR Products
    PCR and qPCR Methods

    Cell Culture

    Shop Cell Culture Products
    Cell Culture

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