Molecular Biology Methods
Shop by Molecular Biology Method
Well-prepared DNA is critical to your experiments and research, so how you isolate and purify your samples is important. Multiple methods exist for disrupting the cells, removing debris, capturing the DNA, removing contaminants, and eluting the DNA for use in downstream applications. Find products for isolation, extraction, purification, and more to complete this step of your process.
Tags or labels, called probes, are added to nucleic acids to enable detection or purification. Labels may include radioactive phosphates, biotin, fluorophores, and enzymes. Fluorescent in situ hybridization (FISH) is used to identify specific DNA sequences and tests can also be performed for pathogen detection. Find labeling and detection kits, spectrophotometers, fluorometers, and more below for your nucleic acid work.
The goal of PCR is the amplification of existing DNA. Ultimately, having multiple copies makes it possible to perform your assays. Choose from a variety of thermal cyclers, polymerases, master mixes, and other PCR systems to support your PCR amplification needs.
Cloning and genetic modification are commonly used to treat genetic defects. The field of genetics can help with DNA manipulation in vivo, altering genes to correct mutations, introduce new genetic information, or remove specific DNA sequences. Shop enzymes and inhibitors, cloning kits, competent cells, and more to help you conduct your cloning and modification research.
Mimicking natural viral processes, transfection artificially introduces nucleic acids to cells, producing various chemical, biological, or physical changes. Although the term transfection was originally used to describe the uptake of viral nucleic acid from a prokaryote‑infecting virus or bacteriophage, the term currently refers to any artificial introduction of foreign nucleic acid into a cell. Search for transfection reagents, electroporation supplies, and more to help effectively conduct your transfection procedures.
The yield from your PCR and molecular biology procedures must be further analyzed and evaluated to confirm the outcome of your assays, especially if the material is intended for other downstream applications. Shop electrophoresis equipment, gel stains, imaging and documentation systems, and more to help ensure the success of your molecular biology assays.
Cell Analysis Methods
Find what you need for flow cytometry, cell imaging, and more.