Buffers and Standards
Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.
The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:
- Red: pH 4.0
- Yellow: pH 7.0
- Blue: pH 10.0
Filtered Search Results
TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents
| Color | Colorless |
|---|---|
| Physical Form | Liquid |
| Chemical Name or Material | Tris-EDTA |
| Grade | Molecular Biology |
| DNase | DNase free |
| Filtered Through | Filtered through a 5-micron filter. |
| ChemAlert Storage Symbol | Gray |
| Name Note | 1X Solution, pH 8.0 |
| CAS | 7732-18-5 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0 |
| Health Hazard 2 | CAUTION! |
| pH | 7.4 to 8.1 |
| Synonym | TE |
| Recommended Storage | RT |
Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™
Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
| Molecular Weight (g/mol) | 238.30 |
|---|---|
| ChEBI | CHEBI:42334 |
| Color | White |
| Chemical Name or Material | HEPES |
| Grade | Molecular Biology |
| SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
| Identification | Pass Test |
| DNase | DNase free |
| Merck Index | 15, 4689 |
| InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
| ChemAlert Storage Symbol | Gray |
| Assay Percent Range | ≥99 % |
| PubChem CID | 23831 |
| Absorbance | 0.01 max. (0.1M solution) at 280nm |
| Percent Purity | ≥99% |
| CAS | 7365-45-9 |
| Protease | Protease free |
| Health Hazard 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
| MDL Number | MFCD00006158 |
| Health Hazard 2 | WARNING! |
| Solubility Information | Soluble in water |
| pH | 5.0 to 6.5 |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
| Molecular Formula | C8H18N2O4S |
| Physical Form | Liquid |
|---|---|
| Recommended Storage | Room temperature |
Hydrogen Peroxide, 50% (Certified), Fisher Chemical
CAS: 7722-84-1 Molecular Formula: H2O2 Molecular Weight (g/mol): 34.014 MDL Number: MFCD00011333 InChI Key: MHAJPDPJQMAIIY-UHFFFAOYSA-N Synonym: oxydol,perhydrol,superoxol,interox,hydrogen dioxide,inhibine,peroxaan,albone,hioxyl,kastone PubChem CID: 784 ChEBI: CHEBI:16240 IUPAC Name: hydrogen peroxide SMILES: OO
| PubChem CID | 784 |
|---|---|
| CAS | 7722-84-1 |
| Molecular Weight (g/mol) | 34.014 |
| ChEBI | CHEBI:16240 |
| MDL Number | MFCD00011333 |
| SMILES | OO |
| Synonym | oxydol,perhydrol,superoxol,interox,hydrogen dioxide,inhibine,peroxaan,albone,hioxyl,kastone |
| IUPAC Name | hydrogen peroxide |
| InChI Key | MHAJPDPJQMAIIY-UHFFFAOYSA-N |
| Molecular Formula | H2O2 |
Traceable™ Conductivity Calibration Standards Certified Reference Material (CRM), NIST tested, Fisherbrand™
Use NIST-traceable reference materials for the highest accuracy available. Traceable™ Conductivity Calibration Standards Certified Reference Material (CRM) offer easy calibration with included instructions and temperature chart.
| Type | Conductivity Standard |
|---|---|
| For Use With (Equipment) | Conductivity Meter |
| Solubility | Soluble in water |
| Color | Clear |
| Packaging | Bottle |
| Chemical Name or Material | Conductivity Standard |
| Certifications/Compliance | ISO 17034 |
| Technique | Calibration of Conductivity Instrument |
| Solution Type | Calibration Solution |
Traceable™ One-Shot™ Conductivity Calibration Standards Certified Reference Material Producer (CRM), Fisherbrand™
Ideal for lab or field applications
| For Use With (Equipment) | Conductivity Meter |
|---|---|
| Solubility | Soluble in water |
| Color | Clear |
| Packaging | 6/Pk. |
| Chemical Name or Material | Conductivity Standard |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
| CAS | 10043-35-3 |
|---|---|
| Synonym | TBE |
Tris Base (White Crystals or Crystalline Powder/Triple-Crystallized), Fisher BioReagents™
For sensitive molecular biology, tissue culture, and electrophoresis methods | CAS: 77-86-1 | C4H11NO3 | 121.136 g/mol
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
| PubChem CID | 6503 |
|---|---|
| CAS | 77-86-1 |
| Molecular Weight (g/mol) | 121.136 |
| ChEBI | CHEBI:9754 |
| SMILES | C(C(CO)(CO)N)O |
| Synonym | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
| IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
| InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
| Molecular Formula | C4H11NO3 |
TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™
Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT
| Name Note | 50X Solution |
|---|---|
| Concentration or Composition (by Analyte or Components) | Tris (24%), Acetic Acid (5.0%), and EDTA (<2%). |
| CAS | 6850-28-8 |
| Protease | Protease free |
| Physical Form | Liquid |
| Grade | Electrophoresis |
| Synonym | TAE |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| DNase | DNase free |
| Recommended Storage | RT |
| Filtered Through | Filtered through a 0.2-micron filter. |
| ChemAlert Storage Symbol | Gray |
Phenol/Chloroform/Isoamyl Alcohol (25:24:1 Mixture, pH 6.7/8.0, Liq.), Fisher BioReagents™
CAS: 108-95-2 Molecular Formula: C6H6O Molecular Weight (g/mol): 94.11 MDL Number: MFCD00002143 InChI Key: ISWSIDIOOBJBQZ-UHFFFAOYSA-N Synonym: carbolic acid,hydroxybenzene,phenic acid,phenylic acid,oxybenzene,benzenol,phenyl hydrate,monophenol,phenyl hydroxide,phenylic alcohol PubChem CID: 996 ChEBI: CHEBI:15882 IUPAC Name: phenol SMILES: OC1=CC=CC=C1
| PubChem CID | 996 |
|---|---|
| CAS | 108-95-2 |
| Molecular Weight (g/mol) | 94.11 |
| ChEBI | CHEBI:15882 |
| MDL Number | MFCD00002143 |
| SMILES | OC1=CC=CC=C1 |
| Synonym | carbolic acid,hydroxybenzene,phenic acid,phenylic acid,oxybenzene,benzenol,phenyl hydrate,monophenol,phenyl hydroxide,phenylic alcohol |
| IUPAC Name | phenol |
| InChI Key | ISWSIDIOOBJBQZ-UHFFFAOYSA-N |
| Molecular Formula | C6H6O |
| Boiling Point | 100°C |
|---|---|
| Color | Colorless |
| Physical Form | Liquid |
| Chemical Name or Material | Tris Buffered Saline |
| Grade | Molecular Biology |
| DNase | DNase free |
| ChemAlert Storage Symbol | Gray |
| Name Note | 10X Solution |
| Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
| CAS | 7732-18-5 |
| Protease | Protease free |
| pH | 7.5 |
| Synonym | TBS |
| Purity Grade Notes | DNase-, RNase- and Protease-Free |
| Recommended Storage | RT |
MES (Fine White Crystals), Fisher BioReagents
CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.25 MDL Number: MFCD00149409 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid,2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate SMILES: O.OS(=O)(=O)CCN1CCOCC1
| PubChem CID | 16218417 |
|---|---|
| CAS | 145224-94-8 |
| Molecular Weight (g/mol) | 213.25 |
| MDL Number | MFCD00149409 |
| SMILES | O.OS(=O)(=O)CCN1CCOCC1 |
| Synonym | 2-(4-Morpholino)ethane Sulfonic Acid,2-(N-Morpholino)ethanesulfonic acid hydrate |
| IUPAC Name | 2-(morpholin-4-yl)ethane-1-sulfonic acid hydrate |
| InChI Key | MIIIXQJBDGSIKL-UHFFFAOYSA-N |
| Molecular Formula | C6H15NO5S |