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  5. Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System

Cytiva Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System

Catalog No. 45-000-925
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Quantity:
For 2000 cm2 Membrane
For 4000 cm2 membrane
Labels 10 μg Nucleic Acid
Labels 5 μg Nucleic Acid
Product Type:
Detection Reagents
Labeling and Detection System
Labeling Module
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Catalog No. Quantity Product Type
45000925 Labels 5 μg Nucleic Acid Labeling Module
45000874 For 4000 cm2 membrane Detection Reagents
45000923 Labels 10 μg Nucleic Acid Labeling and Detection System
45000924 For 2000 cm2 Membrane Detection Reagents
45000922 Labels 5 μg Nucleic Acid Labeling and Detection System
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Catalog No. 45000925 Supplier Cytiva Supplier No. RPN3005
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Description

Includes

45-000-922 and 45-000-923: Labeling reagent; Crosslinker; Control DNA; Blocking agent; ECL detection reagents and ECL Gold hybridization buffer

Use for fast and easy detection of medium-to-high-target amounts. Cytiva Life Sciences™ Amersham™ ECL™ Direct Nucleic Acid Labeling/Detection System are based on the direct labeling of DNA or RNA probes with horseradish peroxidase (HRP) in a simple 20 min chemical reaction

 

    • Direct probe labeling in a 10 min reaction, 1 h from hybridization to detection with ECL Direct, Hybond N+, and Hyperfilm ECL.
    • Eliminates handling, waste, and regulatory issues associated with the use of radioactivity.
    • No need to strip blots before reprobing.
    • For fast and easy detection of medium- to high-target amounts in applications such as colony/plaque screens, dot blots, and PCR product analyses.
    • Consistent results combining strong signals with very low backgrounds.

 

 

Specifications

Content And Storage 2°C to 8°C
For Use With (Application) Colony or plaque screens, dot blots and PCR product analyses
Includes DNA labelling reagent 0.5mL; Glutaraldehyde solution 0.5mL; Water 1.0mL; l Hind III control DNA (10μg/mL) 0.1 ml; Gold hybridization buffer 500mL; Blocking agent 25g
Product Type Labeling Module
Quantity Labels 5 μg Nucleic Acid
Test Time 20 min.