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Competent Cells

Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
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1

Thermo Scientific™ DH5α Competent Cells; High Efficiency

High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors

2

Invitrogen™ One Shot™ MAX Efficiency™ DH5α-T1R Competent Cells  DFS Item

One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the tonA (fhuA) mutation that confers resistance to T1 and T5 phage.

3

Invitrogen™ One Shot™ TOP10 Electrocomp™ E. coli  DFS Item

One Shot TOP10 Electrocomp E. coli cells are ideal for high-efficiency cloning and plasmid propagation. Provided at a transformation efficiency of >1 x 1010 cfu/ μg supercoiled DNA, these cells allow for stable replication of high copy number plasmids.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Unstable DNA No
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 1010 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid May be used for plasmids >20 kb
Reduces Recombination Yes (recA13)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Electrocompetent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome No
4

Invitrogen™ One Shot™ TOP10/P3 Chemically Competent E. coli  DFS Item

One Shot TOP10/P3 Chemically Competent E. coli cells are designed for vector transformation encoding the synthetic supF gene (tyrosine tRNA suppressor) and allow high-quality pCDM8, pcDNA1.1 plasmid, or any other supF-containing vector preparation.

5

Invitrogen™ One Shot™ TOP10 Chemically Competent E. coli  DFS Item

One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.

Shipping Condition Dry Ice
Blue/White Screening Yes (lacZΔM15)
Cloning Methylated DNA Yes (mcrA)
Transformation Efficiency Level High Efficiency (>1 x 109 cfu/μg)
High-throughput Compatibility Low
Improves Plasmid Quality Yes (endA1)
Preparing Unmethylated DNA No
Plasmid High Copy Plasmid
Reduces Recombination Yes (recA1)
T1 Phage - Resistant (tonA) No
Antibiotic Resistance Bacterial Yes (Streptomycin)
Format Tube
Product Type Chemically Competent Cells
Species E. coli (K12)
Product Line One Shot
Contains F' Episome No
6

Invitrogen™ ElectroMAX™ DH10B Cells  DFS Item

ElectroMAX DH10B Cells are electrocompetent E. coli cells offering the highest transformation efficiencies of >1 x 1010 cfu/μg plasmid DNA.

7

MilliporeSigma™ Annexin V-PE Apoptosis Detection Kit

Identifies phosphatidylserine (PS) that translocates from cytoplasmic face of plasma membrane to cell surface during apoptosis.

8

Invitrogen™ MAX Efficiency™ DH10B Competent Cells  DFS Item

MAX Efficiency DH10B Competent Cells are highly efficient E. coli suitable for a variety of molecular biology applications. This strain is one of the most commonly used strains for everyday applications due to key strain features such as high transformation efficiency.

9

Invitrogen™ Tali™ Cell Cycle Kit  DFS Item

Convenient and accurate determination of the percentage of cells in each phase of the cell cycle

10

Invitrogen™ MultiShot™ FlexPlate DH10B T1R Competent Cells  DFS Item

MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.

11

MilliporeSigma™ Novagen™ HMS174 (DE3) Competent Cells

HMS174 strains provide high transformation efficiencies and the recA mutation in a K-12 background. Strain may stabilize certain target genes whose products may cause the loss of the DE3 prophage.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >5 x 106 cfu/μg
12

MilliporeSigma™ Novagen™ BL21 (DE3) pLysS Singles™ Competent Cells

BL21 strains are the most widely used hosts for protein expression from pET recombinants and have the advantage of being deficient in both lon and ompT proteases.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 107 cfu/μg
13

MilliporeSigma™ Novagen™ BL21(DE3) pLysS Competent Cells

BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.

Content And Storage ≤-70°C
Efficiency Guaranteed efficiency: >2 x 107 cfu/μg
14

MilliporeSigma™ Novagen™ Veggie BL21 (DE3) Singles™ Competent Cells

Protease-deficient hosts (BL21) for protein expression; Manufactured free of animal-derived media and components

Content And Storage ≤-70°C
15

Invitrogen™ MAX Efficiency™ DH10Bac Competent Cells  DFS Item

Capable of supporting site-specific recombination between pFastBac™ and bMON14272