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Subcloning Efficiency DH5α Competent Cells are a versatile, chemically competent strain for cloning that provides a transformation efficiency of >1 x 106 cfu/μg plasmid DNA.
High efficiency, chemically competent E. coli cells ideal for construction of gene banks or generation of cDNA libraries using plasmid-derived vectors
Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
Library Efficiency DH5α Competent Cells are a versatile strain of chemically competent cells that are used in many everyday cloning applications.
One Shot TOP10 Chemically Competent E. coli are ideal for high-efficiency cloning and plasmid DNA propagation and are provided at a transformation efficiency of 1 x 109 cfu/μg plasmid DNA.
Résistance aux antibiotiques des bactéries | Yes (Streptomycin) |
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Clonage d’ADN méthylé | Yes (mcrA) |
Type de produit | Chemically Competent Cells |
Plasmide | High Copy Plasmid |
Résistant au phage T1 (tonA) | No |
Espèces | E. coli (K12) |
Conditions d’expédition | Dry Ice |
Compatibilité à haut débit | Low |
Format | Tube |
Sélection bleue / blanche | Yes (lacZΔM15) |
Niveau d’efficacité de la transformation | High Efficiency (>1 x 109 cfu/μg) |
Gamme de produits | One Shot™ |
Contient l’épisome F | No |
Réduit la recombinaison | Yes (recA1) |
Améliore la qualité des plasmides | Yes (endA1) |
Préparation de l’ADN non méthylé | No |
MegaX DH10B T1R Electrocomp Cells are the highest-efficiency electrocompetent cells available. Improved production processes provide a transformation efficiency reaching >3 x 1010 cfu/μg of pUC DNA.
Capable of supporting site-specific recombination between pFastBac™ and bMON14272
One Shot Stbl3 Chemically Competent E. coli are designed for cloning direct repeats found in lentiviral expression vectors as these cells reduce the frequency of homologous recombination of direct repeats found in lentiviral vectors (ViraPower Lentiviral Expression kits) and other retroviral vectors (instability-prone HIV-based plasmids).
One Shot ccdB Survival 2 T1R Competent Cells are suitable for the propagation of plasmids containing the ccdB gene and are designed for use with the Gateway Vector Conversion System and for propagating Gateway destination, donor, and supercoiled entry vectors.
Animal origin-free, chemically defined, protein-free medium
Indicateur rouge de phénol | No Phenol Red |
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Lignée cellulaire | FreeStyle™ CHO-S Cells, CHO |
Type de culture | Suspension Cell Culture |
Type de produit | Expression Medium |
Classification | Chemically Defined, Protein-Free, Serum-Free |
Gamme de produits | Freestyle™, Gibco™ |
Niveau d’endotoxines | ≤0.25 EU/mL, ≤ 0.25 EU/mL |
Niveau de sérum | Serum-Free |
Forme | Liquid |
Glutamine | No Glutamine |
Contenu et stockage | Storage conditions: 2°C to 8°C. Protect from light Shipping conditions: Ambient Shelf life: 12 months from date of manufacture |
ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 1010 cfu/μg plasmid DNA in efficiency.
MAX Efficiency DH5α T1R Phage-Resistant Competent Cells are high efficiency chemically competent cells for generating cDNA libraries and were developed to confer resistance to the lytic bacteriophages T1 and T5.
ElectroMAX Stbl4 Competent Cells are specifically designed for cloning unstable inserts. This strain is a derivative of Stbl2 cells (engineered from JM109/J5 strain).