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PCR Supplies

A variety of products designed for use in various PCR procedures. Includes dNTPs, enzymes, assays, arrays, master mixes, reagents, and kits. Products can be used for standard, real-time, direct, high fidelity, hot start, and long range PCR protocols.
Real Time PCR Products (650)
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Physical Form Liquid
pH 7
Chemical Name or Material Saline-Sodium Citrate
Grade Molecular Biology
DNase DNase free
Recommended Storage RT
Concentration 2.9 to 3.1 M
2

Applied Biosystems™ SYBR™ Select Master Mix  DFS Item

Cost-effective solution for real-time PCR applications.

GC-Rich PCR Performance High
Content And Storage The 2x mix contains SYBR™ GreenER™ dye, AmpliTaq™ DNA Polymerase UP, dNTPs with dUTP/dTTP blend, heat-labile UDG, ROX passive reference dye and optimized buffer components. Contains 1 X 1 mL tube, sufficient for 100, 20 μL reactions (10 μL Master Mix per reaction). Store at 2–8°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).

See user guide for primer concentration recommendations for the StepOne and StepOnePlus systems.
Purity or Quality Grade UP (Ultra Pure)
Form Liquid
Concentration 2X
For Use With (Application) Gene Expression
PCR Method qPCR
Detection Method SYBR
Polymerase AmpliTaq DNA Polymerase
Format Vial
Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Reaction Speed Fast or Standard
Label or Dye SYBR GreenER
Hot Start Built-In Hot Start
Sufficient For 100 Reactions of 20 μL
Product Line SYBR
3

Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG  DFS Item

Is a ready-to-use mixture for high-specificity, real-time quantitative DNA amplification, containing UDG to prevent carryover contamination (1,2).

Shipping Condition Dry Ice
GC-Rich PCR Performance High
Product Type Quantitative PCR SuperMix-UDG
For Use With (Equipment) 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ
Sample Type DNA (Genomic), cDNA
Reaction Speed Standard
For Use With (Application) Gene Expression
PCR Method qPCR
Product Line Platinum
Detection Method Primer-probe
4

Invitrogen™ Taq DNA Polymerase, recombinant  DFS Item

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Shipping Condition Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Standard PCR
PCR Method qPCR,Standard PCR
Exonuclease Activity 5' - 3'
Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start No
Starting Material DNA
5

Thermo Scientific™ Taq DNA Polymerase, native (5 U/μL)

Thermo Scientific Native Taq DNA Polymerase is a highly thermostable DNA polymerase of the thermophilic bacterium Thermus aquaticus.

Polymerase Taq DNA Polymerase
Product Type DNA Polymerase
Concentration 5 U/μL
For Use With (Application) Standard PCR
6

Applied Biosystems™ AmpliTaq™ 360 DNA Polymerase  DFS Item
GREENER_CHOICE

The Applied Biosystems AmpliTaq360 DNA Polymerase, when used with the new enhanced AmpliTaq 360 Buffer and the optional 360 GC Enhancer, amplifies a vast range of DNA sequence contexts.

Fidelity (vs. Taq) 1X
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Overhang 3'-A
Concentration 5 U/μL
Starting Material DNA
Exonuclease Activity 5' - 3'
7

Applied Biosystems™ AmpliTaq Gold™ 360 Buffer Kit  DFS Item
GREENER_CHOICE

The AmpliTaq Gold 360 Buffer Kit is used in conjunction with AmpliTaq Gold 360 DNA Polymerase and is intended for those occasions when the quantity of reagents supplied with the enzyme are not sufficient.

8

Invitrogen™ Taq DNA Polymerase, native  DFS Item

Taq DNA Polymerase is a thermostable enzyme that synthesizes DNA from single-stranded templates in the presence of dNTPs and a primer.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Overhang 3'-A
Concentration 5 U/μL
For Use With (Application) Standard PCR
Exonuclease Activity 5' - 3'
Polymerase Taq DNA Polymerase
Format Stand-alone enzyme
Size (Final Product) 5 kb or less
Reaction Format Separate Components
Product Type DNA Polymerase
Reaction Speed Standard
Hot Start No
Starting Material DNA
9

MilliporeSigma™ Novagen™ NovaTaq™ Hot Start Master Mix Kits

Premixed 2X hot start PCR components for convenience and reproducibility

Content And Storage Ready-to-use 2X mixture of NovaTaq Hot Start DNA Polymerase, ultrapure deoxynucleotides, and reaction buffer with MgCl2, PCR Grade Water, MgCl2 for optimizing Mg2+ concentrations
Product Type Hot start master mix kit
10

Applied Biosystems™ Fast MicroSEQ™ 500 16S rDNA PCR Kit  DFS Item

The Fast MicroSEQ™ 500 16S rDNA PCR kit utilizes new Fast technology and provides bacterial identifications from colony to results in as little as 5 hours using the Veriti™ 96-well Thermal Cycler.

11

Applied Biosystems™ GeneAmp™ 10X PCR Buffer II & MgCl2  DFS Item

Applied Biosystems GeneAmp 10X PCR Buffer II is optimized for use with AmpliTaq DNA Polymerase, resulting in robust PCR amplification.

Polymerase AmpliTaq DNA Polymerase
Fidelity (vs. Taq) 1X
Reaction Format Kit
Product Type PCR Buffer
Concentration 10X
For Use With (Application) Standard PCR
12

Thermo Scientific™ DreamTaq PCR Master Mixes (2X)

Thermo Scientific DreamTaq PCR Master Mix (2X) is a ready-to-use solution containing DreamTaq DNA Polymerase, optimized DreamTaq buffer, MgCl2, and dNTPs.

Shipping Condition Dry Ice
Polymerase DreamTaq DNA Polymerase
GC-Rich PCR Performance Low
Fidelity (vs. Taq) 1X
Reaction Format SuperMix or Master Mix
Reaction Speed Standard
Hot Start No
Overhang 3'-A
For Use With (Application) Standard PCR
13

Applied Biosystems™ AmpErase™ Uracil N-Glycosylase (UNG)  DFS Item

Enzyme removes any uracil incorporated into single- or double-stranded DNA

14

Applied Biosystems™ CLA IdentiFiler™ Plus PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Plus PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

15
Promotions Available

Applied Biosystems™ TaqMan™ Sample-to-SNP™ Kit (200mL Sample prep, 10mL PCR)  DFS Item

Go from Biological Sample to Genotyping Results in less than one hour.

Sample Type Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood
Reaction Speed Fast or Standard
Detection Method Primer-probe