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PCR Cloning Kits

Chemical reagents, kits, and other products used for polymerase chain reaction (PCR) cloning.
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1

Invitrogen™ RNase T1 (1 U/μL)  DFS Item

Invitrogen™ RNase T1, RNA-Grade cleaves 3' of single-stranded G residues and is tested for purity to ensure that no contaminating ribonuclease activities are present that could cleave at unanticipated sites.

2

Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells  DFS Item

Enables rapid cloning of one or more genes into virtually any protein expression system

3

Invitrogen™ Ambion™ T4 RNA Ligase, cloned, 5 U/μL  DFS Item

Catalyzes formation of a phosphodiester linkage between a 5'-phosphoryl-terminated nucleic acid donor and a 3'-hydroxyl-terminated nucleic acid acceptor

4
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Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing

5

Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for subcloning

6

Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit  DFS Item

Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.

7

Invitrogen™ pENTR™/TEV/D-TOPO™ Cloning Kit, with One Shot™ Mach1™-T1R Chemically Competent E. coli  DFS Item

Use highly efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector

8

Invitrogen™ pENTR™/D-TOPO™ Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli  DFS Item

Efficient, 5-minute cloning strategy to directionally clone blunt-end PCR product into vector for entry into Gateway System or MultiSite Gateway System

10

Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, with One Shot™ Mach1™ T1 Phage-Resistant Chemically Competent E. coli  DFS Item

Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for sequencing

11

Invitrogen™ pCR™8/GW/TOPO™ TA Cloning Kit with One Shot™ Mach1™-T1R E. coli  DFS Item

Delivers fast, efficient and reliable cloning into various destination vectors for multitude of applications

12

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with pCR™-Blunt II-TOPO™ Vector, One Shot™ TOP10 Chemically Competent E. coli, and PureLink™ Quick Plasmid Miniprep Kit  DFS Item

Offers the fastest and easiest method for high-efficiency (cloning of blunt-end PCR products amplified with proofreading thermostable polymerases

13

Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, with One Shot™ MAX Efficiency™ DH5α-T1R E. coli  DFS Item

Provides a highly efficient, 5 minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of proofreading-polymerase–amplified blunt-end PCR products into a plasmid vector for sequencing

14

Invitrogen™ GeneRacer™ Kit with SuperScript™ III RT and TOPO TA Cloning™ Kit for Sequencing  DFS Item

Ensures amplification of only full-length transcripts via elimination of truncated messages from amplification process