
PCR Cloning Kits
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Invitrogen™ TOPO™ TA Cloning™ Kit for Subcloning, with One Shot™ TOP10 chemically competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase-amplified PCR products into a plasmid vector for subcloning
Souche bactérienne ou de levures | TOP10 |
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Format | Kit |
Type de produit | Cloning Kit |
Type de cellule | Chemically Competent |
Méthode de clonage | TOPO™-TA |
Contenu et stockage | Box 1: • Topoisomerase I-activated pCR™2.1-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute cloning reaction for the direct insertion of proofreading polymerase-amplified, blunt-ended PCR products into a plasmid vector for sequencing
Résistance aux antibiotiques des bactéries | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Type de produit | PCR Cloning Kit |
Vecteur | TOPO Cloning Vectors |
Gamme de produits | TOPO™ |
Conditions d’expédition | Dry Ice |
Accélérateur | T7, T3 |
Méthode de clonage | Blunt TOPO™ |
Contenu et stockage | Box 1: • Topoisomerase I-activated pCR™4Blunt-TOPO™ vector • Salt solution • dNTPs • Control template • T3, T7, M13 forward, and M13 reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Résistance aux antibiotiques des bactéries | Kanamycin (KanR), Zeocin™ (ZeoR) |
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Format | Kit |
Type de produit | PCR Cloning Kit |
Accélérateur | SP6 |
Méthode de clonage | Blunt TOPO™ |
Contenu et stockage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Invitrogen™ TOPO™ TA Cloning™ Kit for Sequencing, without competent cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of Taq polymerase–amplified PCR products into a plasmid vector for sequencing
Invitrogen™ Zero Blunt™ TOPO™ PCR Cloning Kit, with One Shot™ TOP10 Chemically Competent E. coli cells
Provides a highly efficient, 5-minute, one-step cloning strategy (TOPO Cloning) for the direct insertion of blunt-ended PCR products amplified with proofreading thermostable polymerases into a plasmid vector
Souche bactérienne ou de levures | TOP10 |
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Format | Kit |
Type de produit | PCR Cloning Kit |
Type de cellule | Chemically Competent |
Accélérateur | SP6 |
Méthode de clonage | Blunt TOPO™ |
Contenu et stockage | Box 1: • Linearized and topoisomerase I-activated pCR™Blunt II-TOPO™ vector • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. Box 2: • One Shot™ Chemically Competent or Electrocomp™ E. coli • S.O.C. medium • Supercoiled pUC19 control plasmid Store at -68 to -85°C. |
Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™/Zeo & OmniMAX™2 Competent Cells
Enables rapid cloning of one or more genes into virtually any protein expression system
Invitrogen™ PCR Cloning System with Gateway™ Technology with pDONR™221 & OmniMAX™2 Competent Cells
Enables rapid cloning of one or more genes into virtually any protein expression system
Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit
Used in suspension-adapted cells for transient protein expression. Gibco™ pcDNA™3.4 TOPO™ TA Cloning Kit is a constitutive mammalian expression vector designed to deliver exceptionally high levels of transgene expression.
Résistance aux antibiotiques des bactéries | Ampicillin (AmpR), Kanamycin (KanR) |
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Format | Kit |
Vecteur | TOPO-TA Vectors |
Gamme de produits | TOPO™ |
Accélérateur | T7, SP6 |
Méthode de clonage | TOPO™-TA |
Contenu et stockage | Box 1: • Topoisomerase I-activated pCR™II-TOPO™ vector • PCR buffer • Salt solution • dNTPs • Control template • M13 forward and reverse primers • Control PCR primers • Sterile water Store at -5 to -30°C. All reagents are stable for 6 months when properly stored. |
Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli
Provides a quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into a plasmid vector. Invitrogen™ TA Cloning™ Kit, with pCR™2.1 Vector and One Shot™ TOP10 Chemically Competent E. coli is available in 20- and 40-reaction kit sizes.
Souche bactérienne ou de levures | TOP10 |
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Format | Kit |
Type de produit | Cloning Kit |
Gamme de produits | One Shot™ |
Type de cellule | Chemically Competent E. coli |
Accélérateur | T7 |
Méthode de clonage | TA Cloning |
À utiliser avec (application) | PCR Cloning |
Contenu et stockage | TA Cloning™ kits contain linearized pCR™2.1 vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. The competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector and One Shot™ TOP10F' Chemically Competent E. coli
Provides quick, one-step cloning strategy for directly inserting a Taq-amplified PCR product into plasmid vector
Souche bactérienne ou de levures | TOP10F ́ |
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Format | Kit |
Type de produit | Cloning Kit |
Vecteur | pCRII |
Gamme de produits | One Shot™ |
Comprend | Linearized pCRII vector, ExpressLink T4 DNA ligase, 5X ExpressLink T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, controls, One Shot TOP10F' chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. |
Type de cellule | Chemically Competent E. coli |
Accélérateur | T7, SP6 |
Méthode de clonage | TA Cloning |
À utiliser avec (application) | PCR Cloning |
Contenu et stockage | This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Competent cell kits contain One Shot™ chemically competent E. coli, S.O.C. medium, and a supercoiled control plasmid. Store One Shot™ E. coli at -80°C. Store all other components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |
Invitrogen™ TA Cloning™ Kit, Dual Promoter, with pCR™II Vector, without competent cells
Provides quick, one-step cloning strategy for directly inserting Taq-amplified PCR product into plasmid vector
Souche bactérienne ou de levures | Not Included |
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Format | Kit |
Type de produit | Cloning Kit |
Vecteur | pCRII |
Gamme de produits | TA Cloning™ |
Accélérateur | T7, SP6 |
Méthode de clonage | TA Cloning |
À utiliser avec (application) | PCR Cloning |
Contenu et stockage | This kit contains linearized pCR™II vector, ExpressLink™ T4 DNA ligase, 5X ExpressLink™ T4 DNA ligation buffer, dNTPs, 10X PCR buffer, sterile water, and controls. Store all components at -20°C. All reagents are guaranteed stable for 6 months when properly stored. |