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DNA Extraction and Purification

Kits, reagents, and other supplies for the extraction of DNA from a sample, and its subsequent purification. Products include plasmid miniprep and maxiprep kits, plasmid purification kits, PCR purification kits, and genomic DNA purification kits.
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1

Invitrogen™ PureLink™ HiPure Expi Plasmid Megaprep Kit  DFS Item

The PureLink™ HiPure Plasmid Megaprep Kit also includes vacuum filtration columns that provide bacterial filtration without centrifugation, enabling extremely fast process times.

2

Invitrogen™ PureLink™ HiPure Plasmid Miniprep Kit  DFS Item

The PureLink HiPure Plasmid Miniprep Kit allows isolation of high yields of highly pure plasmid DNA from E.coli in less than 2 hours, without use of any organic solvents or cesium chloride, and contains Low endotoxin levels.

Shipping Condition Room Temperature
Target BAC DNA, Plasmid DNA
Format Kit
Product Type Plasmid MiniPrep Kit
Final Product Type BAC DNA, Plasmid DNA
Sample Type Bacterial Culture
Isolation Technology Anion Exchange Resin
Scale Mini
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Test Time 2 hr.
Product Line PureLink
Prep Scale < 100 μg (Small-Scale) Plasmid DNA
3

Invitrogen™ PureLink™ HiPure Plasmid Midi/Maxiprep Precipitators  DFS Item

Traditional DNA precipitation method of anion exchange isolated plasmid DNA involves isopropanol precipitation followed by centrifugation and drying of DNA which is time consuming and labor intensive.

4

Invitrogen™ PureLink™ HiPure Plasmid Filter Midiprep Kit  DFS Item

PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of highest purity from 25 to 100 mL of overnight bacterial culture.

Shipping Condition Room Temperature
Content And Storage The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
Final Product Type Plasmid DNA
Isolation Technology Anion Exchange Resin
Column Type Filter Column
System Type PureLink™
Purity Transfection Grade
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Test Time 90 min.
Prep Scale 100-200 μg (Medium-Scale) Plasmid DNA
Plasmid <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Target Plasmid DNA
Format Column
Product Type Plasmid Filter MidiPrep Kit
Sample Type Bacterial Culture
Yield 350 μg
Elution Volume 5 mL
Product Line PureLink
5

Invitrogen™ PureLink™ HiPure Plasmid Filter Maxiprep Kit  DFS Item

The PureLink HiPure Plasmid Filter Midiprep Kit is designed to isolate plasmid DNA of the highest purity from 25 to 100 mL of overnight bacterial culture.

Shipping Condition Room Temperature
Content And Storage The PureLink™ HiPure Plasmid Filter Kits include Equilibration Buffer (EQ1), Resuspension Buffer (R3), Lysis Buffer (L7), Precipitation Buffer (N3), RNase A, Wash Buffer (W8), Elution Buffer (E4), TE Buffer (TE), and HiPure Filter Columns. Store all components at room temperature upon receipt.
Final Product Type Plasmid DNA
Isolation Technology Anion Exchange Resin
Column Type Filter Column
System Type PureLink™
Purity Transfection Grade
Scale Maxi
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Test Time 90 min.
Prep Scale > 200 μg (Large-Scale) Plasmid DNA
Plasmid <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Target Plasmid DNA
Format Column
Product Type Plasmid Filter MaxiPrep Kit
Sample Type Bacterial Culture
Yield 1000 μg
Elution Volume 15 mL
Product Line PureLink
6

Invitrogen™ PureLink™ HiPure BAC Buffer Kit  DFS Item

PureLink HiPure BAC Buffer Kit provides additional buffers and reagents for performing BAC (Bacterial Artificial Chromosome) DNA isolation using PureLink HiPure Plasmid Kits.

7

Invitrogen™ PureLink™ Quick Gel Extraction Kit  DFS Item
GREENER_CHOICE

The PureLink™ Quick Gel Extraction Kit allows you to rapidly and efficiently purify DNA fragments from TAE or TBE agarose gels of various percentages.

Shipping Condition Room Temperature
Label or Dye Ethidium Bromide, SYBR Safe
High-throughput Compatibility Not High-throughput Compatible (Manual)
Test Time 30 min.
8

Invitrogen™ PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit  DFS Item

The PureLink™ HiPure Plasmid FP (Filter and Precipitator) Maxiprep Kit isolates plasmid DNA, from cell pellet to ultrapure DNA, in less than one hour.

Content And Storage The PureLink™ HiPure Plasmid FP Maxiprep Kit includes Equilibration Buffer, Resuspension Buffer, Lysis Buffer, Precipitation Buffer, RNase A, Wash Buffer, Elution Buffer, TE Buffer, HiPure Filter Columns, and a PureLink™ HiPure Precipitator Module. The PureLink™ HiPure Precipitator Module includes HiPure Precipitators, and 5 mL and 30 mL syringes. Store all components at room temperature.
Final Product Type Plasmid DNA
Isolation Technology Anion Exchange Resin
Column Type Filter Column
System Type PureLink™
Purity Transfection Grade
High-throughput Compatibility Not High-throughput Compatible (Manual)
For Use With (Application) Next-Generation Sequencing, Transfection, Cloning, Sequencing, Transformation, Nucleic Acid Labeling, PCR, In Vitro Transcription
Test Time 1 hr.
Prep Scale > 200 μg (Large-Scale) Plasmid DNA
Plasmid <40kb, Low Copy Plasmid, High Copy Plasmid, BAC
Target Plasmid DNA
Format Column
Product Type Plasmid FP MaxiPrep Kit
Sample Type Bacterial Culture
Yield 1000 μg
Elution Volume 15 mL
Product Line PureLink
9

Thermo Scientific™ Genomic DNA Purification Kit

Isolate genomic DNA from various sources, including whole blood, serum, cell lines, bacterial cells, plant, and mammalian tissues, with this DNA precipitation system.

10

Thermo Scientific™ Ion 16S™ Metagenomics Kit

The Ion 16S™ Metagenomics Kit is designed for rapid, comprehensive and broad-range analyses of mixed microbial populations using the Ion Torrent™ semiconducter sequencing workflow.

11

Invitrogen™ ChargeSwitch™ Plasmid ER Mini Kit  DFS Item

ChargeSwitch™ Plasmid ER Mini Kit uses magnetic bead-based technology to allow for rapid and efficient purification of plasmid DNA.

12

Invitrogen™ ChargeSwitch™ Plasmid Yeast Mini Kit  DFS Item
GREENER_CHOICE

The ChargeSwitch Plasmid Yeast Mini Kit provides an efficient method for purifying high-quality plasmid DNA from 1 mL of Saccharomyces cerevisiae grown in selective minimal media.

13

Invitrogen™ ChargeSwitch™ Forensic DNA Purification Kit  DFS Item

ChargeSwitch™ Forensic DNA Purification Kit uses magnetic bead technology to allow for rapid and efficient purification of genomic DNA from a variety of forensic samples, including poor or environmentally degraded samples, and is fully validated for Single Tandem Repeat (STR) analysis.

Shipping Condition Room Temperature
Target Genomic DNA
Content And Storage

All components of the ChargeSwitch™ Forensic DNA Purification Kits are shipped at room temperature. Upon receipt, store the Proteinase K at 4°C. Store all other components at room temperature. All components are guaranteed stable for 6 months, if stored properly.

Components:
• ChargeSwitch™ Lysis Buffer (L13)
• ChargeSwitch™ Magnetic Beads
• Proteinase K (20 mg/mL in 50 mM Tris-HCl, pH 8.5, 5 mM CaCl2, 50% glycerol)
• ChargeSwitch™ Purification Buffer (N5)
• ChargeSwitch™ Wash Buffer (W12)
• ChargeSwitch™ Elution Buffer (E5; 10 mM Tris-HCl, pH 8.5)
Format Kit
Product Type Forensic DNA Purification Kit
Sample Type Forensic Sample
Isolation Technology ChargeSwitch Chemistry
High-throughput Compatibility High-throughput Compatible
For Use With (Application) Forensic
Product Line ChargeSwitch
14

Invitrogen™ ChargeSwitch™ Lysis Buffer L13  DFS Item
GREENER_CHOICE

ChargeSwitch Forensic DNA Purification Kit is magnetic bead method designed to accommodate all forensic crime scene sample types for STR profiling.

15

Invitrogen™ ChargeSwitch™ gDNA Micro Tissue Kit  DFS Item

Sample size mouse ear clips of 3-5 mg tissue with a typical yield up to 5 μg.