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    1. Home
    2. Protein Biology Methods
    Overview
    1. Isolation
    2. Purification
    3. Separation
    4. Detection
    Related Resources
    Other Applications

    Protein Biology Methods

    Products for Your Workflow

    Genes direct the production of proteins that play a significant role in diseases such as cancer, Alzheimer’s, and diabetes. Protein biology helps us understand the output of these genes and the way they influence our health. Through protein isolation, purification, separation, and detection, protein biology provides earlier and more accurate diagnoses.

    1. Isolation


    Isolate proteins from your mixture with the products below. This protein biology method will help you start your process and prepare you for future workflow steps. Find protein extraction essentials, dissociation reagents, and cell scrapers, plus additional products and resources.

    Top Categories

    Protein Extraction
    Protein Extraction
    In vitro Protein Expression Systems
    In vitro Protein Expression Systems
    Protease Inhibitors and Phosphatase Inhibitors
    Protease Inhibitors and Phosphatase Inhibitors

    Featured Products

    Cytiva triplePrep Kit

    Catalog Number 45-001-810

    Cytiva triplePrep Kit
    MilliporeSigma™ Novagen™ CytoBuster™ Protein Extraction Reagent

    Catalog Number 710094MI

    MilliporeSigmaâ„¢ Novagenâ„¢ CytoBusterâ„¢ Protein Extraction Reagent
    Thermo Scientific™ B-PER™ with Enzymes Bacterial Protein Extraction Kit

    Catalog Number PI90078

    Thermo Scientificâ„¢ B-PERâ„¢ with Enzymes Bacterial Protein Extraction Kit

    Resources

    MilliporeSigmaâ„¢ BugBusterâ„¢ Master Mix Flyer

    Filename
    milliporesigma-bug-buster-master-mix-flier.pdf
    Size
    277 KB
    Format
    application/pdf

    2. Purification


    Purification is another necessary protein biology method and involves cell lysis, protein binding, washing, and elution. The protocol used will depend on the protein you’re purifying. To get started, shop prepacked columns, products for protein purification, dialysis and desalting, and more. For further information, download our resources.

    Top Categories

    Protein Purification
    Protein Purification
    Dialysis and Desalting
    Dialysis and Desalting
    Dialysis Cassettes
    Dialysis Cassettes

    Featured Products

    Thermo Scientific™ Pierce™ Protein A Magnetic Beads

    Catalog Number PI88845

    Thermo Scientificâ„¢ Pierceâ„¢ Protein A Magnetic Beads
    Cytiva HisTrap™ HP Prepacked Columns

    Catalog Number 45-000-323

    Cytiva HisTrapâ„¢ HP Prepacked Columns
    Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

    Catalog Number PI89877

    Thermo Scientific™ Zeba™ Spin Desalting Columns, Plates, and Cartridges, 7K MWCO, 0.5–100 mL

    Resources

    Thermo Scientific Zeba Desalting Products Brochure

    Filename
    thermo-scientific-zeba-desalting-products-brochure.pdf
    Size
    4 MB
    Format
    application/pdf

    Thermo Scientific Protein Clean-Up Technical Handbook

    Filename
    thermo-scientific-protein-clean-up-technical-handbook-bn0322162.pdf
    Size
    4 MB
    Format
    application/pdf

    Thermo Scientific and Invitrogenâ„¢ Protein Preparation Handbook

    Filename
    thermo-scientific-and-invitrogen-protein-preparation-handbook-bn0526161.pdf
    Size
    5 MB
    Format
    application/pdf

    3. Separation


    Solubility, size, charge, and other protein characteristics are used to separate proteins. These techniques include various forms of chromatography in addition to electrophoresis and dialysis. Find the products you need for each of these methods and more by browsing gels, columns, and reagents. Learn more by downloading our relevant resources.

    Top Categories

    Precast Electrophoresis Gels
    Precast Electrophoresis Gels
    Ion Exchange Columns
    Ion Exchange Columns
    Gel Electrophoresis Reagents and Buffers
    Gel Electrophoresis Reagents and Buffers

    Featured Products

    Invitrogen™ Novex™ Tris-Glycine Mini Protein Gels, 4–20%, 1.0 mm, WedgeWell™ format

    Catalog Number XP04205BOX

    Invitrogen™ Novex™ Tris-Glycine Mini Protein Gels, 4–20%, 1.0 mm, WedgeWell™ format DFS Item
    Thermo Scientific™ PageRuler™ Plus Prestained Protein Ladder, 10 to 250 kDa

    Catalog Number PI26619

    Thermo Scientificâ„¢ PageRulerâ„¢ Plus Prestained Protein Ladder, 10 to 250 kDa
    Cytiva HiPrep™ Q Fast Flow 16/10 Column

    Catalog Number 45-002-071

    Cytiva HiPrepâ„¢ Q Fast Flow 16/10 Column

    Resources

    Thermo Scientific and Invitrogen Protein Gels Welcome Packs Brochure

    Filename
    invitrogen-protein-gel-welcome-packs-brochure-bn20170438.pdf
    Size
    219 KB
    Format
    application/pdf

    4. Detection


    Western blotting and ELISA techniques are frequently used for protein detection. Fluorescence microscopy is also common. Browse related reagents, substrates, and imaging systems to help advance your protein detection protocol. For answers that inform your workflow, download our resources.

    Top Categories

    Western Blot Products
    Western Blot Products
    Derivation and Visualization Reagents
    Derivation and Visualization Reagents
    ELISA Reagents and Kits
    ELISA Reagents and Kits

    Featured Products

    Thermo Scientific™ SuperSignal™ West Pico PLUS Chemiluminescent Substrate

    Catalog Number PI34579

    Thermo Scientificâ„¢ SuperSignalâ„¢ West Pico PLUS Chemiluminescent Substrate
    MilliporeSigma™ Immobilon™-P PVDF Membrane

    Catalog Number IPVH08100

    MilliporeSigmaâ„¢ Immobilonâ„¢-P PVDF Membrane
    Invitrogen™ iBright™ CL1500 Imaging System

    Catalog Number A44240

    Invitrogenâ„¢ iBrightâ„¢ CL1500 Imaging System DFS Item

    Resources

    Thermo Scientific and Invitrogen Western Workflow Solutions Brochure

    Filename
    thermo-scientific-invitrogen-western-workflow-solutions-brochure-bn0916161.pdf
    Size
    12 MB
    Format
    application/pdf

    Thermo Scientific HRP Chemiluminescent Substrates Flyer

    Filename
    thermo-scientific-hrp-chemiluminescent-substrates-brochure-bn1031161.pdf
    Size
    995 KB
    Format
    application/pdf

    Related Resources


    Using AI to Create Proteins from Beyond Nature

    Read About Proteins and AI
    Using AI to Create Proteins from Beyond Nature

    Improvements in Affinity Chromatography

    Read About Affinity Chromatography Improvements
    Improvements in Affinity Chromatography

    Multi-Attribute Method in Therapeutic Protein Manufacturing

    Read About Its Role in Quality Control
    Multi-Attribute Method in Therapeutic Protein Manufacturing

    Power Your Protein Research From Collection to Analysis

    Shop Protein Research Products
    Power Your Protein Research From Collection to Analysis
    Viewing 1-3 of 4

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