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Enzymes and Inhibitors

Restriction enzymes, modifying enzymes, buffering solutions, inhibitors, and substrates for use in clinical, research, and general laboratory procedures.
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1

OPTIZYME™ SmaI, Fisher BioReagents™

Components 10X OPTIZYME™ Buffer 4
Cut Site CCC.GGG
Form Liquid
Product Type SmaI
Storage Buffer 10mM Tris HCl (pH 7.4 at 25°C), 100mM KCl, 1mM EDTA, 1mM DTT, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 30°C
Concentration 10 U/μL
For Use With (Application) >90% of pUC19 DNA fragments can be ligated and re-cut after 50-fold over-digestion with SmaI
2

OPTIZYME™ NotI, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin
Cut Site GC.GGCCGC
Content And Storage Keep container tightly closed
Form Liquid
Product Type NotI
Storage Buffer 20mM Tris HCl (pH 7.8 at 25°C), 100mM NaCl, 0.1mM EDTA, 1mM DTT, 0.02% Triton™ X-100, 0.2mg/mL BSA, 50% Glycerol
pH 7.4
Concentration 10 U/μL
For Use With (Application) >90% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with Not I
3

OPTIZYME™ ClaI (Bsu15I), Fisher BioReagents™

Components >50% Water, 25 to 50% Glycerin
Cut Site AT.CGAT
Content And Storage Keep container tightly closed
Form Liquid
Product Type ClaI (Bsu15I)
Storage Buffer 10mM Tris HCl (pH 8 at 25°C), 50mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
5

OPTIZYME™ HindIII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Potassium Chloride
Cut Site A.AGCTT
Content And Storage Keep container tightly closed
Form Liquid
Product Type HindIII
Storage Buffer 10mM Tris HCl (pH 7.5 at 25°C), 250mM KCl, 1mM DTT, 0.1mM EDTA, 0.2mg/mL BSA, 50% Glycerol
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with HaeIII
9

OPTIZYME™ BglII, Fisher BioReagents™

Components 25 to 50% Water, >50% Glycerin, 1 to 2.5% Sodium Chloride
Cut Site A.GATCT
Content And Storage Keep container tightly closed
Form Liquid
Product Type BglII
Storage Buffer 10mM Tris HCl (pH 8.2 at 25°C), 200mM KCl, 1mM DTT, 0.1mM EDTA, 0.5mg/mL BSA, 50% Glycerol
pH 7.4
Incubator Temperature 37°C
Concentration 10 U/μL
For Use With (Application) >95% of DNA fragments can be ligated and re-cut after 50-fold over-digestion with BglII
13
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Fisher BioReagents™ Proteinase K (Tritirachium album/Molecular Biology)

Content And Storage -20°C
Form Solid
Product Type Proteinase K
Color White
For Use With (Application) Isolation of DNA and RNA from cell and tissue preparations, inactivating many Enzymes, including Endogenous Nucleases
Source Tritirachium album
14

Thermo Scientific™ Psp5II (PpuMI) (10 U/μL)

Cut at RG^GWCCY sites with Psp5II (PpuMI) restriction enzyme, which functions best at 37°C in G buffer (Isoschizomers: PpuMI, PspPPI).

15

Thermo Scientific™ FastDigest SmaI

The FastDigest SmaI restriction enzyme recognizes CCC^GGG sites and cuts best at 37°C in 5-15 minutes using universal FastDigest Buffer (isoschizomers: TspMI, XmaCI; neoschizomer: XmaI).

Sensitive to Heat Inactivation Yes
Product Type Restriction Enzyme
Enzyme Sma I
Methylation Sensitivity CpG Methylation-Sensitive,Not Dam Methylation-Sensitive,Not Dcm Methylation-Sensitive
Research Category Traditional Cloning
Compatible Buffer 10X FastDigest Buffer/FastDigest Green Buffer
Overhang Blunt
Type IIS RE No
Optimal Reaction Temperature 37°C
Inactivation Temperature 65°C
Product Line FastDigest