Antibiotics, Buffers and Supplements

Gibco™ Bacto™ Peptone

Enzymatic digest of protein

Gibco™ Penicillin-Streptomycin (10,000 U/mL) DFS Item

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria

Gibco™ Trypsin-EDTA (0.25%), phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ Bacto™ Tryptone

For use with dehydrated media culture

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

Gibco™ N-2 Supplement (100X) DFS Item

N-2 Supplement (100X)

Kanamycin Sulfate (White Powder), Fisher BioReagents

CAS: 25389-94-0 Molecular Formula: C18H38N4O15S Molecular Weight (g/mol): 582.575 InChI Key: OOYGSFOGFJDDHP-IZQIRFRQSA-N PubChem CID: 134129479 IUPAC Name: (2R,3S,4S,5S,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2S,3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxyoxane-3,4,5-triol;sulfuric acid SMILES: C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)O)O)OC3C(C(C(C(O3)CO)O)N)O)N.OS(=O)(=O)O

Chloramphenicol (Crystalline Powder), Fisher BioReagents

CAS: 56-75-7 Molecular Formula: C11H12Cl2N2O5 Molecular Weight (g/mol): 323.126 InChI Key: WIIZWVCIJKGZOK-RKDXNWHRSA-N Synonym: Chloromycetin, D-(?)-threo-2, 2-Dichloro-N-[?-hydroxy-?-(hydroxymethyl)-?-(4-nitrophenyl)ethyl]acetamide PubChem CID: 5959 ChEBI: CHEBI:17698 IUPAC Name: 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide SMILES: C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]

Gibco™ L-Glutamine (200 mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ Antibiotic-Antimycotic (100X) DFS Item

Anti-mycotic, antibiotic for prevention of cell culture contamination

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ HT Supplement (100X) DFS Item

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Gibco™ 2-Mercaptoethanol DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red DFS Item

Unlike porcine trypsin, free of animal-derived components

Gibco™ Bacto™ Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

Gibco™ Bacto™ Malt Extract

The water-soluble portion of malted barley.

Gibco™ Gurr Buffer Tablets DFS Item

A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ Trypsin-EDTA (0.5%), no phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Gentamicin (50 mg/mL) DFS Item

Water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X) DFS Item

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Gibco™ TrypLE™ Express Enzyme (1X), phenol red DFS Item

Animal origin-free, recombinant enzyme

Gibco™ Hygromycin B (50 mg/mL) DFS Item

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus

Corning™ Cell Culture Phosphate Buffered Saline (1X)

With low endotoxin content. Without calcium and magnesium.

Gibco™ Trypsin-EDTA (0.05%), phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas