Antibiotics, Buffers and Supplements

Gibco™ Penicillin-Streptomycin (10,000 U/mL) DFS Item

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria

Gibco™ Trypsin-EDTA (0.25%), phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ N-2 Supplement (100X) DFS Item

N-2 Supplement (100X)

Gibco™ Bacto™ Peptone

Enzymatic digest of protein

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ ACK Lysing Buffer DFS Item

Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ L-Glutamine (200 mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Gibco™ Antibiotic-Antimycotic (100X) DFS Item

Anti-mycotic, antibiotic for prevention of cell culture contamination

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ 2-Mercaptoethanol DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Ampicillin Sodium Salt (Crystalline Powder), Fisher BioReagents

CAS: 69-52-3 Molecular Formula: C16H21N3NaO4S Molecular Weight (g/mol): 374.411 InChI Key: BSFVNXCYXDYHOD-ZQDFAFASSA-N Synonym: Ampicillin PubChem CID: 131673879 IUPAC Name: (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;molecular hydrogen;sodium SMILES: [HH].CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C.[Na]

Cytiva (Formerly GE Healthcare Life Sciences) HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

Gibco™ Trypsin-EDTA (0.05%), phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Corning™ Cell Culture Phosphate Buffered Saline (1X)

With low endotoxin content. Without calcium and magnesium.

Gibco™ TrypLE™ Express Enzyme (1X), no phenol red DFS Item

Unlike porcine trypsin, free of animal-derived components

Kanamycin Sulfate (White Powder), Fisher BioReagents

CAS: 25389-94-0 Molecular Formula: C18H38N4O15S Molecular Weight (g/mol): 582.575 InChI Key: OOYGSFOGFJDDHP-IZQIRFRQSA-N PubChem CID: 134129479 IUPAC Name: (2R,3S,4S,5S,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2S,3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxyoxane-3,4,5-triol;sulfuric acid SMILES: C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)O)O)OC3C(C(C(C(O3)CO)O)N)O)N.OS(=O)(=O)O

Gibco™ Penicillin-Streptomycin (5,000 U/mL) DFS Item

For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.

Chloramphenicol (Crystalline Powder), Fisher BioReagents

CAS: 56-75-7 Molecular Formula: C11H12Cl2N2O5 Molecular Weight (g/mol): 323.126 InChI Key: WIIZWVCIJKGZOK-RKDXNWHRSA-N Synonym: Chloromycetin, D-(?)-threo-2, 2-Dichloro-N-[?-hydroxy-?-(hydroxymethyl)-?-(4-nitrophenyl)ethyl]acetamide PubChem CID: 5959 ChEBI: CHEBI:17698 IUPAC Name: 2,2-dichloro-N-[(1R,2R)-1,3-dihydroxy-1-(4-nitrophenyl)propan-2-yl]acetamide SMILES: C1=CC(=CC=C1C(C(CO)NC(=O)C(Cl)Cl)O)[N+](=O)[O-]

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) (50 mg/mL) DFS Item

Produced by bacterium Micromonospora rhodorangea and acts by binding ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells

Gibco™ HT Supplement (100X) DFS Item

HT-supplemented medium is suitable for post-selection rescue to overcome the effects of residual intracellular aminopterin.

Gibco™ Amphotericin B DFS Item

Formulation of amphotericin B with sodium deoxycholate as an emulsifying agent to maintain the suspension

Gibco™ Hygromycin B (50 mg/mL) DFS Item

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus

Corning™ Cell Recovery Solution

Allows for the recovery of cells cultured on Corning™ Matrigel™ Basement Membrane Matrix for subsequent biochemical analyses

Gibco™ Gurr Buffer Tablets DFS Item

A convenient way to prepare a pH 6.8 phosphate buffer for dilution of Giemsa stain

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