Antibiotics, Buffers and Supplements
Gibco™ Penicillin-Streptomycin (10,000 U/mL)
For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria
Gibco™ ACK Lysing Buffer
Used for the lysis of red blood cells in samples containing white blood cells, such as EDTA-treated whole blood, buffy coats, and bone marrow
Gibco™ Trypsin-EDTA (0.25%), Phenol red
Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas
Gibco™ Trypan Blue Solution, 0.4%
Routinely used as a cell stain to assess cell viability using the dye exclusion test
Gibco™ Bacto™ Tryptone
Enzymatic digest of casein. Gibco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media.
Gibco™ HEPES (1M)
A zwitterionic organic chemical buffering agent commonly used in cell culture media
Corning™ Matrigel™ hESC-Qualified Matrix
Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.
Gibco™ Antibiotic-Antimycotic (100X)
Anti-mycotic, antibiotic for prevention of cell culture contamination
Gibco™ GlutaMAX™ Supplement
An alternative to L-glutamine, with increased stability that improves cell health
Gibco™ MEM Non-Essential Amino Acids Solution (100X)
Used as a growth supplement for cell culture medium, to increase cell growth and viability
Gibco™ Bacto™ Yeast Extract
Formulation of culture media for cultivation of heterotrophic microorganisms. Gibco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.
Gibco™ 2-Mercaptoethanol (55mM)
A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals
Kanamycin Sulfate (White Powder), Fisher BioReagents
CAS: 25389-94-0 Molecular Formula: C18H38N4O15S Molecular Weight (g/mol): 582.575 InChI Key: OOYGSFOGFJDDHP-IZQIRFRQSA-N PubChem CID: 134129479 IUPAC Name: (2R,3S,4S,5S,6R)-2-(aminomethyl)-6-[(1R,2S,3S,4R,6S)-4,6-diamino-3-[(2S,3R,4S,5R,6R)-4-amino-3,5-dihydroxy-6-(hydroxymethyl)oxan-2-yl]oxy-2-hydroxycyclohexyl]oxyoxane-3,4,5-triol;sulfuric acid SMILES: C1C(C(C(C(C1N)OC2C(C(C(C(O2)CN)O)O)O)O)OC3C(C(C(C(O3)CO)O)N)O)N.OS(=O)(=O)O
Corning™ Matrigel™ GFR Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ TrypLE Express Enzyme (1X), No Phenol Red
Unlike porcine trypsin, free of animal-derived components
Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X)
A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells
Ampicillin Sodium Salt (Crystalline Powder), Fisher BioReagents
CAS: 69-52-3 Molecular Formula: C16H21N3NaO4S Molecular Weight (g/mol): 374.411 InChI Key: BSFVNXCYXDYHOD-ZQDFAFASSA-N Synonym: Ampicillin PubChem CID: 131673879 IUPAC Name: (2S,5R,6R)-6-[[(2R)-2-amino-2-phenylacetyl]amino]-3,3-dimethyl-7-oxo-4-thia-1-azabicyclo[3.2.0]heptane-2-carboxylic acid;molecular hydrogen;sodium SMILES: [HH].CC1(C(N2C(S1)C(C2=O)NC(=O)C(C3=CC=CC=C3)N)C(=O)O)C.[Na]
HyClone™ Trypsin Protease
Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.
Gibco™ Trypsin-EDTA (0.05%), Phenol red
Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas
Gibco™ Penicillin-Streptomycin (5,000 U/mL)
For prevention from bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria.
Corning™ Matrigel™ Membrane Matrix
Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.
Gibco™ Hygromycin B (50mg/mL)
Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus