Antibiotics, Buffers and Supplements

Gibco™ Penicillin-Streptomycin (10,000 U/mL) DFS Item

For prevention of bacterial contamination of cell cultures due to their effective combined action against gram-positive and gram-negative bacteria

Gibco™ Trypsin-EDTA (0.25%), Phenol red DFS Item

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Gibco™ Trypan Blue Solution, 0.4% DFS Item

Routinely used as a cell stain to assess cell viability using the dye exclusion test

Gibco™ N-2 Supplement (100X) DFS Item

N-2 Supplement (100X)

Gibco™ Antibiotic-Antimycotic (100X) DFS Item

Anti-mycotic, antibiotic for prevention of cell culture contamination

Corning™ Matrigel™ hESC-Qualified Matrix

Compatible with mTeSR™1-devloped by StemCell Technologies. Corning™ Matrigel™ hESC-Qualified Matrix is widely accepted as an alternative substrate for the culture of hES cells as well as human induced pluripotent stem (iPS) cells.

Gibco™ MEM Non-Essential Amino Acids Solution (100X) DFS Item

Used as a growth supplement for cell culture medium, to increase cell growth and viability

Gibco™ GlutaMAX™ Supplement DFS Item

An alternative to L-glutamine, with increased stability that improves cell health

BD Bacto™ Dehydrated Culture Media Additive: Yeast Extract

Formulation of culture media for cultivation of heterotrophic microorganisms.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Yeast Extract is supplied in dehydrated form for the convenience of the laboratorian and bioprocessing specialist.

Ampicillin Sodium Salt (Crystalline Powder), Fisher BioReagents

CAS: 69-52-3 Molecular Formula: C16H21N3NaO4S Molecular Weight (g/mol): 374.411 InChI Key: BSFVNXCYXDYHOD-ZQDFAFASSA-N Synonym: Ampicillin PubChem CID: 131673879

Gibco™ L-Glutamine (200mM) DFS Item

Ready-to-use stock solution that acts as a cell culture supplement

Corning™ Matrigel™ Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

BD Bacto™ Dehydrated Culture Media Additive: Tryptone

Enzymatic digest of casein.  BD Difco™ Bacto™ Dehydrated Culture Media Additive: Tryptone is part of a large selection of dehydrated media.

Corning™ Matrigel™ GFR Membrane Matrix

Produces a biologically active matrix material resembling the mammalian cellular basement matrix. Corning™ Matrigel™ GFR Basement Membrane Matrix provides a physiologically relevant environment for studies of cell morphology, biochemical function, migration, invasion, and gene expression.

HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

Gibco™ HEPES (1M) DFS Item

A zwitterionic organic chemical buffering agent commonly used in cell culture media

Gibco™ TrypLE Express Enzyme (1X), No Phenol Red DFS Item

Unlike porcine trypsin, free of animal-derived components

Gibco™ Insulin-Transferrin-Selenium (ITS -G) (100X) DFS Item

A basal medium supplement to reduce the amount of fetal bovine serum (FBS) needed to culture cells

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

Gibco™ Geneticin™ Selective Antibiotic (G418 Sulfate) (50mg/mL) DFS Item

Produced by bacterium Micromonospora rhodorangea and acts by binding ribosome, thus inhibiting protein synthesis in both prokaryotic and eukaryotic cells

Gibco™ Trypsin-EDTA (0.05%), Phenol red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Gibco™ Hygromycin B (50mg/mL) DFS Item

Water-soluble antibiotic purified from bacterium Streptomyces hydroscopicus

Kanamycin Sulfate (White Powder), Fisher BioReagents

CAS: 25389-94-0 Molecular Formula: C18H38N4O15S Molecular Weight (g/mol): 582.575 InChI Key: OOYGSFOGFJDDHP-IZQIRFRQSA-N PubChem CID: 134129479

Gibco™ Trypsin-EDTA (0.5%), No Phenol Red DFS Item

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Corning™ DMSO (Dimethyl Sulfoxide)

For cryopreservation of cells along with glycerol, to prevent damage to cell membrane during freezing

Gibco™ 2-Mercaptoethanol (55mM) DFS Item

A potent reducing agent used in cell culture medium to prevent toxic levels of oxygen radicals

Gibco™ Molecular Probes™ Gentamicin DFS Item

Water-soluble antibiotic drug originally purified from the fungus Micromonospora purpurea

Gibco™ B-27™ Supplement (50X), minus vitamin A DFS Item

B-27 Supplement (50X), minus vitamin A

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