Additional Cell Culture Reagents and Supplements
Cytiva HyClone™ Dulbecco's Phosphate Buffered Saline, Solution
Buffer cell culture with PBS in solution.
Cytiva HyClone™ Phosphate Buffered Saline (PBS)
Buffer cell culture growth with PBS
Cytiva HyClone™ 100mM Sodium Pyruvate Solution
Filter sterilized. HyClone™ 100mM Sodium Pyruvate solution is an easily accessible carbohydrate source that may also have protective effects against hydrogen peroxide.
Gibco™ B-27™ Supplement (50X), serum free
The original Gibco B-27 Supplement, optimized for serum-free culture of neurons, supports the growth and viability of neurons and many different cell types.
Corning™ Nu-Serum™ Growth Medium Supplement
Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture
Corning™ Nu-Serum™ IV Growth Serum Replacements
Provides a low-serum alternative to newborn calf, fetal bovine, and other sera used for cell culture
Cytiva HyClone™ HEPES Solution
Maintains physiological pH in cell culture, despite changes in carbon dioxide concentration (produced by cellular respiration). HyClone™ HEPES Solution is part of high-performance line of nutritional and growth supplements.
MilliporeSigma™ Poly-L-Lysine Solution
Used as coating to enhance cell attachment and adhesion to both plasticware and glass surfaces
MilliporeSigma™ Polybrene Infection/Transfection Reagent
A highly efficient method of gene transfer into mammalian cells leveraging infection with retroviral vectors.
Gibco™ Poly-D-Lysine
Poly-D-Lysine is an extracellular matrix used to facilitate neural cell adhesion to tissue culture-treated plastic and glass surfaces. When used on Nunc plastics with B-27 supplements or the B-27 Plus Neuronal Culture System, Poly-D-Lysine maintains cell viability and normal growth characteristics in multiple neuronal cell types.
MilliporeSigma™ EmbryoMax™ 0.1% Gelatin Solution
may be used for routine mouse embryonic stem cell culture applications.
Corning™ Hank's Balanced Salt Solution, 10X
Buffer for maintaining physiological pH of cells maintained in non-CO2 atmospheric conditions
Cytiva HyClone™ AdvanceSTEM™ Dulbecco's Phosphate Buffered Saline, ES Cell Qualified
Gibco™ N-2 Supplement (100X)
N-2 Supplement is a versatile serum-free cell culture supplement that supports the growth and viability of various cell types.
Gibco™ Poloxamer 188 Non-ionic Surfactant (10%)
Poloxamer 188 is a non-ionic surfactant used to control shear forces in suspension cultures, reduce foaming in stirred cultures, and minimize cell attachment to glass surfaces. This product comes as a 10% solution, sterile-filtered and ready for use in cell culture applications.