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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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Cytiva HyClone™ Trypsin Protease

Derived from porcine pancreas. HyClone™ Trypsin Protease is used to disaggregate adherent cells from substrate or surface to which they are attached.

EDGE
Content And Storage Store frozen
Sterilization Method 0.1 μm Sterile-filtered
Form Liquid
Shelf Life 24 Months
Source Porcine pancreas
2

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

EDGE
3

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

EDGE
4

Gibco™ TrypLE™ Express Enzyme (1X), phenol red  DFS Item
GREENER_CHOICE

Animal origin-free, recombinant enzyme

Shipping Condition Room Temperature
Chelators EDTA
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality Check and Record
Sterility Sterile-filtered
Source Animal origin-free
Cell Type Mammalian Cells
Remarks Room Temperature Stable
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin-free
Culture Type Adherent Cell Culture
Shelf Life 24 Months
Product Line Gibco
5

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type Collagenase
Form Lyophilized
Shelf Life 24 Months
Sterility Non-sterile
6

MilliporeSigma™ PluriSTEM™ Dispase-II Solution

a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.

7

Gibco™ StemPro™ Accutase™ Cell Dissociation Reagent  DFS Item

StemPro Accutase is a gentle cell dissociation solution for routine detachment of cells from tissue culture plasticware or adhesion coated plasticware.

8

Invitrogen™ Accumax - Cell Aggregate Dissociation Medium

Dissociate cell clumps for cell counting, viral transfection assays, cell sorting, and flow cytometry as well as bioreactor scale-up.

10

MilliporeSigma™ Chemicon™ Accumax™ Cell Detachment Solution

A cell detachment solution of proteolytic, collagenolytic and DNAse enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

11

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

12

Corning™ Accutase™ Cell Detachment Solution, Liquid

Efficient, with gentle detachment of adherent cells within minutes

EDGE
Content And Storage Store at -20°C.
Form Liquid
Formulation 1 X Accutase enzymes prepared in Dulbeccos PBS (0.2g/L KCl, 0.2g/L KH2PO5, 8g/L NaCl, and 1.15g/L Na2HPO4) containing 0.5 mm EDTA·4Na and 3 mg/L Phenol Red.
Sterility Sterile
13

Gibco™ Trypsin-EDTA (0.05%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas.

Chelators EDTA
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Form Liquid
Tested For In vitro Bioassay
Classification Animal Origin
Shelf Life 24 Months
Osmolality 270 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Product Line Gibco
Cell Type Mammalian Cells
14

Gibco™ Trypsin-EDTA (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, irradiated mixture of proteases derived from porcine pancreas

Chelators EDTA
Content And Storage Storage conditions: -5°C to -20°C
Shipping conditions: Wet ice
Shelf life: 24 months from date of manufacture
Form Liquid
Tested For In vitro Bioassay
Osmolality 270 - 320 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red,EDTA
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
15

Gibco™ TrypLE™ Select Enzyme (1X), no phenol red  DFS Item

Gibco™ TrypLE™ Select is an animal origin free, recombinant enzyme for dissociating a wide range of adherent mammalian cells.

Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Phenol Red
Shelf Life 24 Months
Concentration 1 X