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Dissociation Reagents

Reagents designed to dissociate cells from surface substrates and from other cells; suitable for use with tissues and adherent cells; various types and strengths available.
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2

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium, Magnesium or Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

Content And Storage -20°C
Product Type Cell Culture Dissociation Reagent
Form Liquid
Without Additives No Calcium,No Magnesium,No Sodium Bicarbonate
pH 7 to 7.6
Sterility Sterile
3

Gibco™ Cell Dissociation Buffer, enzyme-free, Hanks' Balanced Salt Solution  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free Hank's Balanced Salt Solution (HBSS).

4

Gibco™ Trypsin (0.25%), phenol red  DFS Item
GREENER_CHOICE

Made from trypsin powder, an irradiated mixture of proteases derived from porcine pancreas

Shipping Condition Wet Ice
Chelators No EDTA
Content And Storage Storage conditions: -5 to -20°C.
Shelf life: 24 months from date of manufacture.
Form Liquid
Tested For In vitro Bioassay
Concentration 1 X
Osmolality 280 - 310 mOsm/kg
Sterility Sterile-filtered
Source Animal Origin
Cell Type Mammalian Cells
Sterilization Method Sterile-filtered
With Additives Phenol Red
Product Type Cell Culture Dissociation Reagent
Classification Animal Origin
Shelf Life 24 Months
Product Line Gibco
5

Gibco™ Soybean Trypsin Inhibitor, powder  DFS Item

Trypsin inhibited at a molar ratio of 1:1 and chymotrypsin and plasmin to lesser extent

6

Gibco™ Collagenase, Type I, powder  DFS Item

Cleaves bond between neutral amino acid (X) and glycine in sequence Pro-X-Gly-Pro, which is found with high frequency in collagen

Shipping Condition Room Temperature
Content And Storage Storage conditions: 2°C to 8°C. Protect from light.
Shipping conditions: Room temperature
Shelf life: 24 months from date of manufacture
Product Type Collagenase
Form Lyophilized
Shelf Life 24 Months
Sterility Non-sterile
7

Corning™ CellStripper Dissociation Reagent

Non-enzymatic cell dissociation solution gently dislodges adherent cells in culture

8

Corning™ 0.05% Trypsin/0.53mM EDTA in HBSS w/o Calcium and Magnesium

Used to enzymatically release adherent cells from tissue culture plates for passaging.

9

Corning™ 0.25% Trypsin, 0.1% EDTA in HBSS w/o Calcium, Magnesium and Sodium Bicarbonate

Used to enzymatically release adherent cells from tissue culture plates for passaging.

10

Gibco™ Defined Trypsin Inhibitor  DFS Item
GREENER_CHOICE

Animal origin-free

11

Gibco™ Trypsin/EDTA Solution (TE)  DFS Item
GREENER_CHOICE

With EDTA and phenol red

12

MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x)

Used to dissociate primary cells, tissues, and tumors with an increased plating efficiency. MilliporeSigma™ Enzyme Free Cell Dissociation Solution PBS Based (1x) is PBS based formulation of chelating agents that contain agents to stabilize their activity on cells.

Content And Storage Store at room temperature or at 4°C.
Product Type Cell Culture Dissociation Reagent
Form Liquid
Formulation Chelating and stabilizing agents
Shelf Life 2 Years
Concentration 1 X
For Use With (Application) Cell Dissociation
Sterility Sterile
13

Gibco™ Cell Dissociation Buffer, enzyme-free, PBS  DFS Item

Cell Dissociation Buffer is a membrane-filtered, isotonic, and enzyme-free solution of salts, chelating agents, and cell-conditioning agents in calcium-free and magnesium-free phosphate-buffered saline (PBS).

14

MilliporeSigma™ Chemicon™ Accutase™ Cell Detachment Solution

useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

15

MilliporeSigma™ PluriSTEM™ Dispase-II Solution

a rapid and effective, yet gentle, agent for separating many tissues and cells grown in vitro.