missing translation for 'onlineSavingsMsg'
Learn More
  1. Home
  2. Products
  3. Cell Culture Media, Supplements, and Reagents
  4. Cell Culture Reagents, Antibiotics and Supplements
  5. Additional Cell Culture Reagents and Supplements
  6. 15250061

Gibco™ Trypan Blue Solution, 0.4%
GREENER_CHOICE

Catalog No. 15250061
Encompass
Change view
Click to view available options
Quantity:
100 mL
1 product options available for selection
Product selection table with 1 available options. Use arrow keys to navigate and Enter or Space to select.
Catalog No. Quantity
15250061 100 mL
Use arrow keys to navigate between rows. Press Enter or Space to select a product option. 1 options available.
1 options
Catalog No. 15250061 Supplier Gibco™ Supplier No. 15250061
Only null left

Description

Routinely used as a cell stain to assess cell viability using the dye exclusion test

  • Performed while counting cells with hemocytometer during routine subculturing, but can be performed any time cell viability needs to be determined quickly and accurately
  • Dye exclusion test is based upon concept that viable cells do not take up impermeable dyes (like Trypan Blue), but dead cells are permeable and take up the dye
  • Produced at a cGMP-compliant facility located in Paisley, Scotland, UK, registered with the FDA as a medical device manufacturer and is certified to the ISO 13485 standard

Order Info

Shipping Condition: Room Temperature

Specifications

Color Blue
Content And Storage Storage conditions: 15 – 30°C
Shipping conditions: Ambient
Shelf life: 36 months from date of manufacture
Cell Type Mammalian Cells
Concentration 0.004
Detection Method Colorimetric
Form Liquid
Product Type Trypan Blue
Sterility Sterile-filtered
For Use With (Application) Cell Counting
Quantity 100 mL
Shipping Condition Room Temperature
Show More Show Less

Frequently Asked Questions (FAQs)

How do I quench any fluorescence outside of my live cells and leave my intracellular staining intact?

There are a number of reagents used to quench fluorescence on the outside of live cells or in the media (background fluorescence). Our Backdrop Background Suppressor reagents are a great option to quench blue, green, and red fluorescent dyes, various media components and fluorescent proteins. Another commonly used reagent is the cell impermeant colorimetric dye, Trypan blue.

How is Gibco Trypan Blue Solution, 0.4% (Cat. No. 15250061) sterilized?

Trypan Blue is filter sterilized through a 0.22 µm filter.
What is Trypan Blue Solution, 0.4% dissolved in?

It is a 0.85% saline solution.
How long can cells be exposed to trypan blue before all cells are stained with the dye?

Unfortunately, there is no definitive answer to this question. The rate at which the cell-impermeant dye is absorbed depends on the cell type, their state of health, nourishment, engulfment activity, etc.

What causes trypan blue to form a precipitate?

Exposure to light may degrade the dye and these contaminants may promote precipitation. Trypan blue can also form orange/red fibrous aggregates if exposed to refrigeration or freezing temperatures.

How does trypan blue solution stain viable cells?

Trypan blue is a cell impermeant stain used to estimate the number of dead cells in a viable population. Its utility is based on the fact that it is a charged dye and does not enter cells unless the membrane is compromised. Live (viable) cells exclude the dye but dead (non-viable) cells or cells with a compromised membrane are stained an intense blue.
Prior to staining with trypan blue solution, do the cells need to be washed to remove the culture medium?

Trypan blue will bind to serum proteins as well as cellular proteins, which may result a high level of background staining. If the background is too dark, cells should be pelleted and resuspended in protein-free medium, buffer, or normal saline prior to counting.
Can I combine trypan blue staining with fluorescence staining?

It depends on the type of fluorescent stain used on the cells. Trypan blue is a cell-impermeant chromophore that can quench fluorescence. It may quench fluorescent staining on the surface of live cells or internal fluorescent staining in dead cells.
Can trypan blue solution be used to differentiate between apoptotic and necrotic cells?

Trypan blue will stain cells that have a compromised membrane. It cannot differentiate between compromised membranes caused by apoptosis or necrosis.
Do you have a protocol for cell counting via trypan blue exclusion?

Yes, you can find the protocol at the following link (https://www.thermofisher.com/us/en/home/references/gibco-cell-culture-basics/cell-culture-protocols/trypan-blue-exclusion.html)
How much Trypan Blue solution should I add to my cell suspension before counting?

We recommend adding 0.1 mL of 0.4% Trypan Blue stock solution to 0.1 mL of cells. However, depending upon the sample and instrumentation, the ratio of 0.4% Trypan Blue solution to the volume of cells can be varied from 1: 10 to 1:1 (v/v) so that the final concentration of Trypan Blue is 0.04% - 0.2%.

For Research Use Only. Not for use in diagnostic procedures.