
Real Time PCR Products




Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix for qPCR
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
Shipping Condition | Wet Ice |
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Polymerase | Dual-Lock Taq DNA Polymerase |
Format | Tube |
Product Type | Real Time PCR SYBR Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 7 Flex, QuantStudio™ Absolute Q Digital PCR System, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA |
Reaction Speed | Fast or Standard |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Product Line | PowerUP™, SYBR™ |
Detection Method | SYBR |
Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM)
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Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.
Shipping Condition | Room Temperature |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 60 X |
Product Type | Gene Expression Assay |
Reaction Speed | Fast |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
GC-Rich PCR Performance | High |
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Purity or Quality Grade | LD (Low DNA) |
Concentration | 2X |
For Use With (Application) | Gene Expression |
PCR Method | qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
Product Type | Real Time PCR SYBR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Product Line | SYBR™ |
Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits
The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Shipping Condition | Dry Ice |
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GC-Rich PCR Performance | Medium |
No. of Reactions | 100 Reactions |
Product Type | Mycoplasma Detection |
Form | Liquid |
Sample Type | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
Reaction Speed | Standard |
Label or Dye | FAM |
For Use With (Application) | Microbial Detection |
PCR Method | Multiplex qPCR |
Target Organism Class | Mollicutes, mycoplasma |
Product Line | MycoSEQ™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG
TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.
Shipping Condition | Wet Ice |
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Polymerase | AmpliTaq Gold DNA Polymerase |
GC-Rich PCR Performance | High |
Product Type | Universal PCR Master Mix |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7000 System, 7300 System, 7500 System, 7700 System, 7900HT System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, GeneAmp 9600, StepOne™, Standard Mode, StepOnePlus™, Standard Mode |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Genotyping,miRNA analysis |
PCR Method | qPCR |
Product Line | TaqMan™ |
Detection Method | Primer-probe |
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
Shipping Condition | Wet Ice |
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GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 2 X |
Form | Liquid |
Concentration | 2X |
For Use With (Application) | Gene Expression,DNA Quantitation,Presence/Absence,miRNA analysis |
PCR Method | qPCR |
Detection Method | Primer-probe |
Polymerase | AmpliTaq Fast DNA Polymerase |
Product Type | Fast Advanced Master Mix |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio Absolute Q Digital PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
Sample Type | DNA (Genomic), cDNA |
Reaction Speed | Fast |
Product Line | TaqMan™ |
Applied Biosystems™ TaqPath™ 1-Step RT-qPCR Master Mix, CG
TaqPath 1-Step RT-qPCR Master Mix, CG, is designed for robust and reproducible one-step pathogen detection and gene expression workflows. The single-tube 4X formulation contains thermostable MMLV reverse transcriptase, dNTPs, UNG, ROX dye, and thermostable Fast DNA polymerase.
Polymerase | Fast DNA Polymerase |
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Reverse Transcriptase | M-MLV |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System, 7500 Fast Dx System, QuantStudio™ Dx |
Form | Liquid |
Sample Type | RNA, cDNA, DNA |
Reaction Speed | Fast or Standard |
Hot Start | Built-In Hot Start |
Concentration | 4X |
For Use With (Application) | Microbial Detection,DNA Quantitation,Presence/Absence |
PCR Method | 1-step RT-qPCR |
Product Line | TaqPath™ |
Detection Method | Primer-probe |
Applied Biosystems™ MycoSEQ™ Mycoplasma Detection Kit, with PrepSeq™ sample preparation, 100 Reactions
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Invitrogen™ Uracil DNA Glycosylase
Removes uracil residues from sugar moiety of single- and double-stranded DNA without destroying phosphodiester backbone
Applied Biosystems™ TaqMan™ Exogenous Internal Positive Control Reagents
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TaqMan™ Exogenous Internal Positive Control Reagents contain a pre-optimized internal positive control (IPC) with pre-designed primers and TaqMan™ probe.
GC-Rich PCR Performance | Low |
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Format | Tube(s) |
Internal Probe Modification | TAMRA™ Quencher (3'), VIC™ (5') |
Product Type | Exogenous Internal Positive Control Reagent |
Reaction Speed | Standard |
Green Features | Sustainable packaging |
PCR Method | qPCR |
Product Line | TaqMan™ |
Applied Biosystems™ Custom TaqMan™ Gene Expression Assay, FAM
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Applied Biosystems Custom TaqMan Gene Expression assays are the custom version of our predesigned TaqMan assays for quantitative real-time PCR.
Shipping Condition | Room Temperature |
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Content And Storage | 1 tube containing a 20X (S and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers). Store at -15 to -25°C. |
Product Type | Custom TaqMan Gene Expression |
For Use With (Equipment) | 7500 System, 7500 Fast System, 7900HT System, StepOne™, StepOnePlus™, ViiA™ 7 System, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio™ 12k Flex |
Green Features | Sustainable packaging |
Product Line | TaqMan™ |
Applied Biosystems™ Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
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Offers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”
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Human ACTB (Beta Actin) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
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Content And Storage | Control Primer-Probe Set(s) |
Form | Liquid |
Gene Symbol | ACTB |
Green Features | Sustainable packaging |
Amplicon Size | 171 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_001101.2 |
Target | β-actin |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |
Applied Biosystems™ Human TBP (TATA-box binding Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
Human TBP (TATA-box binding Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
For Use With (Application) | Gene Expression |
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PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Applied Biosystems™ Human PPIA (Cyclophilin A) Endogenous Control (FAM™/MGB probe, non-primer limited)
Human PPIA (Cyclophilin A) Endogenous Control (FAM™/MGB probe, non-primer limited)
For Use With (Application) | Gene Expression |
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PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Applied Biosystems™ Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
Human RPLPO (Large Ribosomal Protein) Endogenous Control (FAM™/MGB probe, non-primer limited)
Shipping Condition | Room Temperature |
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Content And Storage | Control Primer-Probe Set(s) |
Gene Symbol | RPLPO |
Green Features | Sustainable packaging |
Amplicon Size | 105 |
Concentration | 20X |
For Use With (Application) | Gene Expression |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Accession Number | NM_053275.3 |
Target | RPLPO |
Format | Tube |
Label or Dye | FAM |
Species | Human |
Product Line | FAM™, TaqMan™ |