Real Time PCR Reagents and Kits

Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
- (49)
- (1)
- (10)
- (9)
- (69)
- (6)
- (1)
- (99)
- (17)
- (3)
- (3)
- (3)
- (3)
- (26)
- (4)
- (3)
- (21)
- (6)
- (21)
- (18)
- (5)
- (1)
- (1)
- (15)
- (15)
- (46)
- (5)
- (2)
- (32)
- (10)
- (2)
- (2)
- (5)
- (1)
- (1)
- (6)

Applied Biosystemsâ„¢ MycoSEQâ„¢ Plus Mycoplasma Detection Kits
The MycoSEQâ„¢ Plus Mycoplasma Detection Kit is a TaqManâ„¢-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Medium |
No. of Reactions | 100 Reactions |
Product Type | Mycoplasma Detection |
Form | Liquid |
Sample Type | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
Reaction Speed | Standard |
Label or Dye | FAM |
For Use With (Application) | Microbial Detection |
PCR Method | Multiplex qPCR |
Target Organism Class | Mollicutes, mycoplasma |
Product Line | MycoSEQâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Content And Storage | Store in freezer (-5° to -30°C). |
---|---|
Label or Dye | ROX |
Detection Method | Primer-probe |
Invitrogenâ„¢ EXPRESS One-Step Superscriptâ„¢ qRT-PCR Kit, universal
Kit incorporates proven Invitrogenâ„¢ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Polymerase | Taq DNA Polymerase |
---|---|
Format | Tube |
Product Type | qRT-PCR Kit |
For Use With (Equipment) | 7500 Fast System, 7500 System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, BioRad CFX96â„¢, StepOnePlusâ„¢, Fast Mode, StepOnePlusâ„¢, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ |
Sample Type | RNA |
Reaction Speed | Fast or Standard |
PCR Method | 1-step RT-qPCR |
Product Line | SuperScriptâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ SuperScriptâ„¢ III Platinumâ„¢ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Taq DNA Polymerase |
Product Type | One-Step qRT-PCR Kit |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | Platinumâ„¢, SuperScriptâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ SARS-CoV-2, Flu A/B, RSV RNA Control
The TaqMan SARS-CoV-2, Flu A/B, RSV RNA Control contains targets specific to the SARS-CoV-2, influenza A/B, and RSV genomic regions targeted by the TaqMan SARS-CoV-2, FluA/B RSV RT-PCR Assay Kit.
Applied Biosystemsâ„¢ TaqCheckâ„¢ SARS-CoV-2 Control
The Applied Biosystemsâ„¢ TaqCheckâ„¢ SARS-CoV-2 Control is a synthetic RNA positive control that contains target sequences for the SARS-CoV-2 S and N gene assays included in the TaqCheckâ„¢ SARS-CoV-2 Fast PCR Assay.
6-Tamra NHS/DMSO
The single-isomer preparations of TAMRA are most important for high-resolution techniques such as DNA sequencing and capillary electrophoresis
Applied Biosystemsâ„¢ Precision Plate Holder for Plates, for 7500 Fast System
Precision plate holder for 7500 fast system
Applied Biosystemsâ„¢ TaqManâ„¢ MicroRNA Reverse Transcription Kit
Provides the necessary components for 200 reactions of optimal performance in TaqManâ„¢ MicroRNA Assays.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | High |
Fidelity (vs. Taq) | 10 X |
Reverse Transcriptase | MultiScribeâ„¢ |
Final Product Type | cDNA |
Concentration | 10X |
For Use With (Application) | miRNA analysis |
PCR Method | 2-step RT-qPCR |
Detection Method | Primer-probe |
Size (Final Product) | 200 bp or less |
Reaction Format | Separate Components |
Product Type | MicroRNA Reverse Transcription Kit |
Sample Type | RNA |
Reaction Speed | Fast |
Optimal Reaction Temperature | 42°C |
Product Line | TaqManâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ PreAmp Master Mix Kit
TaqManâ„¢ PreAmp Master Mix Kit contains TaqManâ„¢ PreAmp Master Mix and TaqManâ„¢ Gene Expression Master Mix.
Content And Storage | Contains 2X TaqMan PreAmp Master Mix (1 mL), TaqMan™ Gene Expression Master Mix (5 mL), and TaqMan™ PreAmp Master Mix Kit Quick Reference Card. Store master mixes at 2-8°C. Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA). |
---|---|
For Use With (Equipment) | 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System |
Sample Type | cDNA |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ Power SYBRâ„¢ Green RNA-to-CTâ„¢ 1-Step Kit
The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.
Shipping Condition | Wet Ice |
---|---|
Purity or Quality Grade | UP (Ultra Pure) |
Reverse Transcriptase | ArrayScriptâ„¢ UP |
For Use With (Application) | Gene Expression |
PCR Method | 1-step RT-qPCR |
Detection Method | SYBR |
Polymerase | AmpliTaq Gold DNA Polymerase |
Passive Reference Dye | ROX (Pre-mixed) |
For Use With (Equipment) | 7500 Fast System, 7500 System, 7900HT System, QuantStudioâ„¢ 12k Flex, QuantStudioâ„¢ 3, QuantStudioâ„¢ 5, QuantStudioâ„¢ 6 Flex, QuantStudioâ„¢ 7, StepOneâ„¢, StepOnePlusâ„¢, ViiAâ„¢ 7 System |
Sample Type | RNA |
Reaction Speed | Standard |
Label or Dye | SYBR Green |
Run Time | Standard |
Product Line | RNA-to-CTâ„¢, SYBRâ„¢ |
Applied Biosystemsâ„¢ TaqManâ„¢ Sample-to-SNPâ„¢ Kit (20 mL sample prep, 10 mL PCR)
Go from Biological Sample to Genotyping Results in less than one hour.
Polymerase | AmpliTaq DNA Polymerase |
---|---|
For Use With (Equipment) | 7300 System, 7500 Fast System, 7500 System, 7900HT Fast System, GeneAmp 9700, StepOneâ„¢, Standard Mode, Veriti Thermal Cycler, StepOneâ„¢ Fast Mode, StepOneâ„¢ Standard Mode |
Sample Type | Buccal Sample, Cell Cultures, Hair with Follicle, Leaf Punch or Needle, Seed Chip, Tail Biopsy (Mouse), Tail Biopsy (Rat), Tissue (FFPE), Whole Blood |
Reaction Speed | Fast or Standard |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ resDNASEQâ„¢ Quantitative CHO DNA Kit, 100 reactions
This quantitative PCR (qPCR)-based kit, part of the resDNASEQâ„¢ Quantitative CHO DNA System, allows users to detect sub-picogram amounts of residual DNA from CHO cell line in under 5 hours.