Real Time PCR Master Mixes
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Applied Biosystems™ Absolute Q™ MAP16 Plate Kit and 1-Step RT-dPCR Master Mix
A key component of the QuantStudio Absolute Q Digital PCR System which is powered by the patented microfluidic array plate (MAP) technology that enables consistent and automated sample separation.
Applied Biosystems™ Absolute Q™ Universal DNA Digital PCR Master Mix (5X)
Absolute Q Universal DNA Digital PCR Master Mix is compatible with a broad range of applications and over 20 million Applied Biosystems predesigned TaqMan Assays, eliminating the need to design custom assays.
Thermo Scientific™ Maxima Probe qPCR Master Mixes
Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.
Thermo Scientific™ Maxima Probe qPCR Master Mix (2X), with separate ROX vial
Optimize qPCR with probe chemistry using standard cycling protocols with these ready-to-use qPCR master mixes.
| Polymérase | Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | BioRad CFX96™, BioRad iCycler iQ, BioRad iQ5, BioRad MyiQ, MJ Chromo4, Eppendorf MasterCycler RealPlex, Corbett RotorGene, Bio-Rad CFX384 Touch™, Roche LightCycler 480, Cepheid SmartCycler, MJ Opticon |
| Vitesse de réaction | Standard |
| Format | Tube |
| Type de produit | qPCR Master Mix |
| Gamme de produits | Maxima |
| Type d’échantillon | DNA, dsDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Multiplex Master Mix for qPCR (No ROX)
TaqMan Fast Virus 1-Step Multiplex Master Mix (No ROX) is designed to enable dye flexibility for multiplexing up to four different RNA/DNA targets for high-throughput RT-qPCR.
| Thermostabilité | Not Applicable |
|---|---|
| Capacité multiplex | Up to 4-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 6 Pro, QuantStudio™ 7 Flex, QuantStudio™ 7 Pro, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | None |
| Type d’échantillon | RNA, DNA |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| Hot Start | Built-In Hot Start |
| Spécificité cible | RNA only, Both RNA and DNA |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
| Thermostabilité | 8 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 5-plex |
| GC-Rich PCR Performance | Medium |
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | ROX (Pre-mixed) |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 4X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| Forme | Liquid |
| Contenu et stockage | Store at -15°C to -25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Polymérase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Hot Start | Antibody-based |
| Gamme de produits | TaqPath |
| Comprend | dUTP |
| Durée de conservation | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Spécificité cible | RNA only, Both RNA and DNA |
Applied Biosystems™ TaqPath™ DuraPlex™ 1-Step RT-qPCR Master Mix, no ROX™
Applied Biosystems TaqPath DuraPlex 1-Step RT-qPCR Master Mix is a benchtop-stable, single-tube master mix optimized for rapid, sensitive, and reproducible detection of viral and bacterial pathogens even in the presence of PCR inhibitors.
| Thermostabilité | 8 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Up to 6-plex |
| GC-Rich PCR Performance | Medium |
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast or Standard |
| Colorant de référence passive | None |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| Forme | Liquid |
| Contenu et stockage | Store at –15 to –25°C. Store at room temperature (24°C) for 1 week after first use and 4°C for 1 month after first use. |
| Polymérase | Applied Biosystems™ AmpliTaq™ DNA Polymerase with Hot Start |
| Format | Single-Tube |
| Hot Start | Antibody-based |
| Gamme de produits | TaqPath |
| Comprend | dUTP |
| Durée de conservation | Two years from date of manufacture, with guaranteed minimum shelf life of ≥1 year upon receipt |
| Spécificité cible | RNA only, Both RNA and DNA |
Invitrogen™ Platinum™ Quantitative PCR SuperMix-UDG w/ROX
qPCR SuperMixes with ROX for ABI instruments are ready-to-use reaction mixes for high-specificity, real-time amplification of cDNA, genomic DNA or plasmid DNA.
| Polymérase | Platinum Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | Primer-probe |
| Fidélité (par rapport à Taq) | 2 X |
| À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT System |
| Vitesse de réaction | Standard |
| Type de produit | Quantitative PCR SuperMix-UDG |
| Gamme de produits | Platinum |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ TaqMan™ Fast Advanced Master Mix for qPCR
TaqMan Fast Advanced Master Mix delivers accurate quantification and dependability in less time on an array of qPCR instrument platforms.
| Thermostabilité | 72 hr., Assembled Reaction |
|---|---|
| Capacité multiplex | Duplex |
| GC-Rich PCR Performance | High |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Flex, QuantStudio 7 Pro, StepOne, StepOnePlus, ViiA 7 System |
| Vitesse de réaction | Fast |
| Type de produit | Fast Advanced Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Type d’échantillon | DNA (Genomic), cDNA |
| InhibitorToleranceSimple | High |
| Méthode PCR | qPCR |
| Concentration | 2X |
| Conditions d’expédition | Wet Ice |
| À utiliser avec (application) | Gene Expression,DNA Quantification,Presence/Absence,miRNA analysis |
| Forme | Liquid |
| Polymérase | AmpliTaq Fast DNA Polymerase |
| Fidélité (par rapport à Taq) | 2 X |
| Gamme de produits | TaqMan |
| Comprend | dUTP, UNG/UDG |
| Spécificité cible | cDNA or gDNA only |
Applied Biosystems™ TaqMan™ Multiplex Master Mix
Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (less than 40 minutes).
| Polymérase | AmpliTaq Fast DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | Primer-probe |
| Fidélité (par rapport à Taq) | 2 X |
| Vitesse de réaction | Fast |
| Type de produit | Multiplex Master Mix |
| Gamme de produits | TaqMan |
| Type d’échantillon | DNA |
| Méthode PCR | Multiplex qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression,Virus Detection |
Applied Biosystems™ TaqMan™ PreAmp Master Mix (cDNA pre-amplification for qPCR)
TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System |
| Type de produit | PreAmp Master Mix |
| Gamme de produits | TaqMan |
| Type d’échantillon | cDNA |
| Méthode PCR | qPCR |
| À utiliser avec (application) | Pre-amplification |
Applied Biosystems™ Power SYBR™ Green PCR Master Mix
Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| À utiliser avec (équipement) | 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System |
| Vitesse de réaction | Standard |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | LD (Low DNA) |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Polymérase | AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD |
| Gamme de produits | SYBR |
| Marqueur ou colorant | SYBR Green |
Applied Biosystems™ SYBR™ Green Universal Master Mix
Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient, single-tube format.
| Polymérase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Méthode de détection | SYBR |
| Vitesse de réaction | Standard |
| Format | Tube |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Gamme de produits | SYBR |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Marqueur ou colorant | SYBR Green |
Applied Biosystems™ TaqMan™ Fast Virus 1-Step Master Mix for qPCR
TaqMan Fast Virus 1-Step Master Mix is designed for fast, highly sensitive real-time RT-PCR, even in the presence of challenging PCR inhibitors.
| Thermostabilité | Not Applicable |
|---|---|
| Capacité multiplex | Up to 3-plex |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT System, QuantStudio 12k Flex, QuantStudio 3, QuantStudio 5, QuantStudio 6 Flex, QuantStudio 6 Pro, QuantStudio 7 Pro, QuantStudio 7 Flex, StepOne, StepOnePlus, ViiA 7 System |
| Vitesse de réaction | Fast or Standard |
| Type de produit | 1-Step Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Type d’échantillon | Total RNA, Viral DNA, Viral RNA, mRNA |
| InhibitorToleranceSimple | High |
| Méthode PCR | 1-step RT-qPCR |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection,Gene Expression,DNA Quantification,Presence/Absence |
| Forme | Liquid |
| Polymérase | AmpliTaq Fast DNA Polymerase |
| Format | Tube |
| Hot Start | Built-In Hot Start |
| Gamme de produits | TaqMan |
| Spécificité cible | RNA only, Both RNA and DNA |
| Transcriptase inverse | M-MLV |
Applied Biosystems™ Fast SYBR™ Green Master Mix
Designed for speed, SYBR™ Green Master Mix provides a fast, reliable, and cost-effective solution for RT-PCR application without compromising sensitivity, specificity, dynamic range, or PCR efficiency.
| GC-Rich PCR Performance | High |
|---|---|
| Méthode de détection | SYBR |
| À utiliser avec (équipement) | 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode |
| Vitesse de réaction | Fast |
| Type de produit | Real Time PCR SYBR Master Mix |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | UP (Ultra Pure) |
| Type d’échantillon | DNA (Genomic), cDNA |
| Méthode PCR | qPCR |
| Concentration | 2X |
| À utiliser avec (application) | Gene Expression |
| Polymérase | AmpliTaq Fast DNA Polymerase, AmpliTaq Fast UP |
| Gamme de produits | SYBR |
| Marqueur ou colorant | SYBR Green |