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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
$129.00 - $4,825.00
Specifications
Detection Method | Detection Method: SYBR; PCR Method: qPCR |
---|---|
Sample Type | DNA |
For Use With (Application) | Real Time PCR (qPCR) |
Storage Requirements | Store at 2° to 8°C |
Catalog Number | Mfr. No. | No. of Reactions | Quantity | Price | Quantity | ||||
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Catalog Number | Mfr. No. | No. of Reactions | Quantity | Price | Quantity | ||||
A25741
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Applied Biosystems™
A25741 |
100 reactions | 1mL |
Each for $129.00
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A25742
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Applied Biosystems™
A25742 |
500 reactions | 5mL |
Each for $548.00
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A25743
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Applied Biosystems™
A25743 |
5000 reactions | 50mL |
Each for $4,825.00
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A25778
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Applied Biosystems™
A25778 |
5000 reactions | 10 x 5mL |
Each for $4,825.00
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|
A25780
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Applied Biosystems™
A25780 |
500 reactions | 5 x 1mL |
Each for $596.00
|
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A25918
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Applied Biosystems™
A25918 |
1000 reactions | 10 x 1mL |
Each for $1,144.00
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A25776
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Applied Biosystems™
A25776 |
1000 reactions | 2 x 5mL |
Each for $1,026.00
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A25777
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Applied Biosystems™
A25777 |
2500 reactions | 5 x 5mL |
Each for $2,460.00
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A25779
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Applied Biosystems™
A25779 |
200 reactions | 2 x 1mL |
Each for $246.00
|
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Description
Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.
- A dual hot-start mechanism for excellent specificity
- Highly reproducible CTs over a broad dynamic range
- Inclusion of UDG to help prevent carryover contamination
- Stability of pre-assembled reactions for up to 72 hours
- Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism:
- Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
- PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
- In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification
Tight reproducibility in CTs over a wide dynamic range:
- PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
- In addition, this master mix provides efficient amplification over 6 logs of sample input
- Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality
UDG included for carryover contamination control:
- The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives
Stability of pre-assembled reactions:
- The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
- This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1
Broad instrument compatibility:
- PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
- It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
- For optimal results, recommended primer concentrations are 300 to 800nM
Order Info
Shipping Conditions: Wet Ice
Specifications
Detection Method: SYBR; PCR Method: qPCR | |
DNA | |
Real Time PCR (qPCR) | |
Store at 2° to 8°C |
For Research Use Only. Not for use in diagnostic procedures.