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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix DFS Item

Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.

$57.50 - $2,115.00

Specifications

Detection Method SYBR
Speed Fast or standard (reaction)
Sample Type DNA general
For Use With (Equipment) 7300 system, 7500 Fast Dx system, 7500 Fast system, 7700 system, 7900HT Fast system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Real-Time PCR systems or Thermal Cyclers, Roche LightCycler 480, StepOne™ system, StepOnePlus™ system, ViiA™ 7 system
For Use With (Application) Real time PCR (qPCR)
View More Specs

Disclaimers:

For Research Use Only. Not for use in diagnostic procedures.



Products ${productFamilyLength}
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
A25741 Applied Biosystems™
LSA25741
100 Reactions Each for $57.50
A25742 Applied Biosystems™
LSA25742
500 Reactions Each for $243.50
A25743 Applied Biosystems™
LSA25743
5000 Reactions Each for $2,077.50
A25776 Applied Biosystems™
LSA25776
1000 rxn(s) Each for $458.00
A25777 Applied Biosystems™
LSA25777
2500 rxn(s) Each for $1,092.50
A25778 Applied Biosystems™
LSA25778
5000 rxn(s) Each for $2,115.00
A25780 Applied Biosystems™
LSA25780
500 rxn(s) Each for $265.00
A25779 Applied Biosystems™
LSA25779
200 Reactions Each for $109.50
A25918 Applied Biosystems™
LSA25918
1000 rxn(s) Each for $507.00
Specifications

Description

Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.

  • A dual hot-start mechanism for excellent specificity
  • Highly reproducible CTs over a broad dynamic range
  • Inclusion of UDG to help prevent carryover contamination
  • Stability of pre-assembled reactions for up to 72 hours
  • Compatibility with most real-time qPCR instruments

Exceptional specificity with dual hot-start mechanism:
  • Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
  • PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
  • In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification

Tight reproducibility in CTs over a wide dynamic range:
  • PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
  • In addition, this master mix provides efficient amplification over 6 logs of sample input
  • Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality

UDG included for carryover contamination control:
  • The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives

Stability of pre-assembled reactions:
  • The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
  • This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1

Broad instrument compatibility:
  • PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
  • It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
  • For optimal results, recommended primer concentrations are 300 to 800nM

Order Info

Shipping Conditions: Wet Ice

Description & Specifications

Specifications

SYBR
DNA general
Real time PCR (qPCR)
Fast or standard (reaction)
7300 system, 7500 Fast Dx system, 7500 Fast system, 7700 system, 7900HT Fast system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Real-Time PCR systems or Thermal Cyclers, Roche LightCycler 480, StepOne™ system, StepOnePlus™ system, ViiA™ 7 system
2 to 8°C