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Applied Biosystems™ PowerUp™ SYBR™ Green Master Mix 
Pre-formulated, optimized, universal 2X master mix for real-time PCR workflows.
$57.50 - $2,115.00
Specifications
| Detection Method | SYBR |
|---|---|
| Speed | Fast or standard (reaction) |
| Sample Type | DNA general |
| For Use With (Equipment) | 7300 system, 7500 Fast Dx system, 7500 Fast system, 7700 system, 7900HT Fast system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Real-Time PCR systems or Thermal Cyclers, Roche LightCycler 480, StepOne™ system, StepOnePlus™ system, ViiA™ 7 system |
| For Use With (Application) | Real time PCR (qPCR) |
Disclaimers:
For Research Use Only. Not for use in diagnostic procedures.
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
|---|---|---|---|---|---|---|---|---|---|
| Catalog Number | Mfr. No. | No. of Reactions | Price | Quantity | |||||
| A25741 |
Applied Biosystems™
LSA25741 |
100 Reactions |
Each for $57.50
|
|
A25742 |
Applied Biosystems™
LSA25742 |
500 Reactions |
Each for $243.50
|
|
A25743 |
Applied Biosystems™
LSA25743 |
5000 Reactions |
Each for $2,077.50
|
|
A25776 |
Applied Biosystems™
LSA25776 |
1000 rxn(s) |
Each for $458.00
|
|
A25777 |
Applied Biosystems™
LSA25777 |
2500 rxn(s) |
Each for $1,092.50
|
|
A25778 |
Applied Biosystems™
LSA25778 |
5000 rxn(s) |
Each for $2,115.00
|
|
A25780 |
Applied Biosystems™
LSA25780 |
500 rxn(s) |
Each for $265.00
|
|
A25779 |
Applied Biosystems™
LSA25779 |
200 Reactions |
Each for $109.50
|
|
A25918 |
Applied Biosystems™
LSA25918 |
1000 rxn(s) |
Each for $507.00
|
|
Description
Coupled with user-supplied primer sets and template, PowerUp™ SYBR™ Green Master Mix is designed to amplify targets for accurate gene expression analysis.
- A dual hot-start mechanism for excellent specificity
- Highly reproducible CTs over a broad dynamic range
- Inclusion of UDG to help prevent carryover contamination
- Stability of pre-assembled reactions for up to 72 hours
- Compatibility with most real-time qPCR instruments
Exceptional specificity with dual hot-start mechanism:
- Specificity is paramount in obtaining high-quality data in SYBR™ Green reactions and can be enhanced through control of the Taq DNA polymerase at lower temperatures before stringent primer binding
- PowerUp™ SYBR™ Green Master Mix uses the Dual-Lock™ Taq DNA polymerase enzyme that combines two unique hot-start mechanisms to help control its activity and to prevent undesirable early activity of the polymerase at low temperatures
- In an experiment evaluating 24 different primer sets, PowerUp™ SYBR™ Green Master Mix generated single melt curves 100% of the time, consistent with highly specific amplification
Tight reproducibility in CTs over a wide dynamic range:
- PowerUp™ SYBR™ Green Master Mix demonstrates excellent reproducibility over a wide dynamic range for a variety of targets tested
- In addition, this master mix provides efficient amplification over 6 logs of sample input
- Unlike competitor mixes that can exhibit inhibition at high cDNA inputs and low correlation coefficients over a dynamic range experiment, PowerUp™ SYBR™ Green Master Mix efficiency is maintained over the full dynamic range to help deliver maximal confidence in data quality
UDG included for carryover contamination control:
- The inclusion of uracil-DNA glycosylase (UDG) and dUTP in the PowerUp™ SYBR™ Green Master Mix enables the degradation of any previously amplified PCR products, helping prevent contamination of subsequent qPCR reactions and possible false positives
Stability of pre-assembled reactions:
- The tight control of the Dual-Lock™ Taq DNA polymerase in PowerUp™ SYBR™ Green Master Mix enables reactions to be assembled up to 72 hours prior to cycling without impacting data
- This consistency allows users to have confidence and flexibility in the use of PowerUp™ SYBR™ Green Master Mix across numerous workflow scenarios1
Broad instrument compatibility:
- PowerUp™ SYBR™ Green Master Mix can be used in either standard or fast cycling mode and is compatible with all Applied Biosystems™ real-time PCR instruments2
- It is also compatible with the Bio-Rad IQ™5 and CFX96™/CFX384™ systems, Roche LightCycler™ LC480, and Stratagene™ MX3005P™ systems
- For optimal results, recommended primer concentrations are 300 to 800nM
Order Info
Shipping Conditions: Wet Ice
Specifications
| SYBR | |
| DNA general | |
| Real time PCR (qPCR) |
| Fast or standard (reaction) | |
| 7300 system, 7500 Fast Dx system, 7500 Fast system, 7700 system, 7900HT Fast system, Bio-Rad CFX Connect™, Bio-Rad CFX384 Touch™, Real-Time PCR systems or Thermal Cyclers, Roche LightCycler 480, StepOne™ system, StepOnePlus™ system, ViiA™ 7 system | |
| 2 to 8°C |