Real Time PCR Products

Real Time PCR Products

A range of products specifically designed for real-time PCR workflows, a process that monitors the amplification of a targeted DNA molecule during the PCR reaction, not at its end, as in conventional PCR. Products include assays, reagents, kits, and master mixes.
Real Time PCR Master Mixes (234)
Real Time PCR Assays (177)
Real Time PCR Reagents and Kits (150)

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1 – 30 of 341 results
1

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Sample Type DNA, dsDNA
Detection Method SYBR
2

Thermo Scientific™ ABsolute Blue QPCR Mix, SYBR Green, low ROX

Incorporates an inert blue dye to significantly enhance the contrast between reagent and plastic, making verification of master mix dispensing quick, easy, and foolproof.

Sample Type DNA, dsDNA
Detection Method SYBR
3

Thermo Scientific™ Verso 1-step RT-qPCR Kit, with separate ROX vial

The Verso RT enzyme delivers high processivity and can be used at temperatures up to 57°C, delivering effective transcription through difficult RNA secondary structures.

PCR Method 1-step RT-qPCR
Detection Method Primer-Probe
4

Applied Biosystems™ Human B2M (Beta-2-Microglobulin) Endogenous Control (FAM™/MGB probe, non-primer limited)  DFS Item

Human B2M (Beta-2-Microglobulin) Endogenous Control (FAM™/MGB probe, non-primer limited)

PCR Method 2-step RT-qPCR
Detection Method Primer-Probe
5

Applied Biosystems™ Precision Plate Holder for Plates, for 7500 Fast System  DFS Item

Precision plate holder for 7500 fast system

6

Applied Biosystems™ Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)  DFS Item

Mouse ACTB (Actin, Beta) Endogenous Control (VIC™/MGB probe, primer limited)

7

Applied Biosystems™ TaqMan™ Universal PCR Master Mix, no AmpErase™ UNG  DFS Item

TaqMan™ Universal PCR Master Mix, No AmpErase™ UNG is the ideal reagent solution when you need a Master Mix for multiple 5' nuclease DNA applications.

Primer Probe Compatibility TaqMan™
Polymerase AmpliTaq Gold DNA Polymerase
GC-Rich PCR Performance High
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Detection Method Primer-Probe
8

Applied Biosystems™ TaqMan™ MicroRNA Reverse Transcription Kit  DFS Item

Provides the necessary components for 200 reactions of optimal performance in TaqMan™ MicroRNA Assays.

Sample Type RNA
9

Applied Biosystems™ TaqMan™ Gene Expression Master Mix  DFS Item

Precise and reliable real-time qPCR quantification over 9 logs of linear dynamic range with Applied Biosystems™ TaqMan™ Gene Expression Master Mix.

Passive Reference Dye ROX (Pre-mixed)
Reaction Speed Standard
PCR Method qPCR
Product Line TaqMan™
Detection Method Primer-Probe
10

Applied Biosystems™ Power SYBR™ Green PCR Master Mix  DFS Item

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Product Type Real Time PCR SYBR Master Mix
Passive Reference Dye ROX (Pre-mixed)
Sample Type DNA (Genomic), cDNA
Label or Dye SYBR™ Green
Concentration 2X
Detection Method SYBR
11

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit  DFS Item

Features a synthetic RNA transcript that lacks homology to current annotated biological sequences

12

Applied Biosystems™ TaqMan™ Genotyping Master Mix  DFS Item

Optimized for end-point fluorescence detection in SNP genotyping applications.

Detection Method Primer-Probe
13

Applied Biosystems™ Power SYBR™ Green RNA-to-CT™ 1-Step Kit  DFS Item

The novel formulation reduces false positive results caused by primer dimers, significantly improves data accuracy, and provides reliable detection of low abundance targets.

Primer Probe Compatibility Unlabeled Oligos
Sample Type RNA
14

Invitrogen™ TaqMan™ PreAmp Cells-to-CT™ Kit  DFS Item

Perform gene expression analysis directly from limited or small numbers of cultured cells without RNA purification

15

Applied Biosystems™ Path-ID™ qPCR Master Mix  DFS Item

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

PCR Method qPCR
Detection Method Primer-Probe
16

Applied Biosystems™ Path-ID™ qPCR Master Mix with Manual  DFS Item

Ambion™ Path-ID™ qPCR Master Mix is a convenient enzyme, buffer, and dNTP mix for TaqMan™ probe-based, real-time PCR detection of DNA targets.

PCR Method qPCR
Detection Method Primer-Probe
17

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit  DFS Item

Perform microRNA (miRNA) expression profiling directly from cultured cells without RNA purification

18

Applied Biosystems™ TaqMan™ RNA-to-CT™ 1-Step Kit  DFS Item

Delivers consistent RNA target quantification for a wide variety of assays and is validated with Applied Biosystems™ comprehensive library of TaqMan™ Gene Expression assays.

PCR Method 1-step RT-qPCR
Detection Method Primer-Probe
19

Applied Biosystems™ TaqMan™ Multiplex Master Mix  DFS Item

Applied Biosystems™ TaqMan™ Multiplex Master Mix is formulated to amplify up to 4 targets in a single reaction, delivering shorter run times (<40 minutes).

Polymerase AmpliTaq™ Fast DNA Polymerase
Sample Type DNA
Concentration 2X
PCR Method Multiplex qPCR
Detection Method Primer-Probe
Product Line TaqMan™
20

Applied Biosystems™ TaqMan™ PreAmp Master Mix  DFS Item

TaqMan™ PreAmp Master Mix pre-amplifies small amounts of cDNA without introducing amplification bias to the sample.

For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, StepOnePlus™, ViiA™ 7 System
Sample Type cDNA
For Use With (Application) Pre-Amplification
Detection Method Primer-Probe
21