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Invitrogen™ Uracil DNA Glycosylase

Catalog No. 18054015
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18054015 100 units
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Catalog No. 18054015 Supplier Invitrogen™ Supplier No. 18054015
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Description

Removes uracil residues from sugar moiety of single- and double-stranded DNA without destroying phosphodiester backbone

Uracil DNA Glycosylase (uracil-N-glycosylase) removes uracil residues from the sugar moiety of single- and double-stranded DNA without destroying the phosphodiester backbone, preventing its use as a hybridization target or as a template for DNA polymerases.

Also known as uracil-N-glycosylase.

  • Does not remove uracil from RNA
  • Purified from E. coli expressing E. coli ung gene on plasmid
  • One unit catalyzes the release of 1nmol of free uracil from 3H-poly(dU) in 1 hr. at 37°C

Helps to eliminate carryover contamination in PCR, resulting in fewer false positive results for cloning PCR fragments

Recommended Storage

Store at -5deg; to -30°C

Specifications

Content And Storage Store in freezer (-5° to -30°C).
Form Liquid
PCR Method qPCR
Polymerase DNA Polymerase
Quantity 100 units
Shipping Condition Approved for shipment on Wet or Dry Ice
For Use With (Application) Standard PCR, Real Time PCR (qPCR)

Frequently Asked Questions (FAQs)

What is UDG?

UDG (uracil-DNA-glycosylase) refers to a superfamily of enzymes comprising six sub-families. Family I UDG enzymes are called UNG, after the uracil-N-glycosylase gene. The terms UDG and UNG are commonly used interchangeably because they perform the same function in qPCR, namely, to remove Uracil from dU-containing DNA to prevent carryover contamination from previous PCRs. See the following link: https://www.thermofisher.com/uk/en/home/life-science/pcr/real-time-pcr/real-time-pcr-learning-center/real-time-pcr-basics/what-is-ung-udg.html

For Research Use Only. Not for use in diagnostic procedures.