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  7. Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Applied Biosystems™ Power SYBR™ Green PCR Master Mix

Catalog No. p-4927003
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1 x 5 mL
2 x 5 mL
5 x 5 mL
10 x 5 mL
1 x 50 mL
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Catalog No. Quantity
4368577 1 x 1 mL
4367659 1 x 5 mL
4368706 2 x 5 mL
4368702 5 x 5 mL
4368708 10 x 5 mL
4367660 1 x 50 mL
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Catalog No. 4368577 Supplier Applied Biosystems™ Supplier No. 4368577
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Description

Includes

SYBR™ Green 1 Dye, AmpliTaq Gold DNA Polymerase LD, dNTPs with dUTP/dTTP blend, Passive Reference 1, and optimized buffer components

Requires

Template and primers

Offers superior sensitivity and reproducibility, detecting as few as two copies of a target gene over a broad range of template concentrations.

Get superior nucleic acid quantitation with Applied Biosystems™ Power SYBR™ Green Master Mix.

  • Everything needed for SYBR™ Green dye-based PCR amplification and detection in a convenient single-tube format
  • Power SYBR™ Green PCR Master Mix contains all of the components (excluding the template and primers) for superior SYBR™ Green reagent-based real-time PCR
  • The optimized formulation contains highly purified AmpliTaq Gold™ DNA Polymerase, LD to offer increased sensitivity
  • Contains a blend of dTTP/dUTP that is compatible with AmpErase™ UNG to minimize carryover contamination
  • Includes a proprietary version of ROX™ dye, an internal passive reference, to normalize non-PCR–related fluorescence fluctuations
  • This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error and sample evaporation
  • Power SYBR™ Green reagent-based PCR chemistry easily replaces the existing SYBR™ Green PCR Master Mix in Applied Biosystems™ protocols using the same setup and thermal cycling conditions
  • Store at -20°C

Order Info

Shipping Condition: Wet Ice

Specifications

Concentration 2X
Content And Storage The 2x mix contains SYBR™ Green 1 Dye, AmpliTaq Gold™ DNA Polymerase LD, dNTPs with dUTP⁄dTTP blend, Passive Reference 1 and optimized buffer components. Contains 1 X 1 mL vial, sufficient for 40 reactions at 50 μL. Store at -20°C.

Guaranteed minimum shelf life is 60 days (exact expiry date printed on product and CofA).
Detection Method SYBR
Format Tube
GC-Rich PCR Performance High
PCR Method qPCR
Polymerase AmpliTaq Gold DNA Polymerase, AmpliTaq Gold LD
Reaction Speed Standard
For Use With (Equipment) 7300 System, 7500 System, 7700 System, 7900HT Fast System,Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™ 12k Flex, QuantStudio™ 3, QuantStudio™ 5, QuantStudio™ 6 Flex, QuantStudio™ 7, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System
Label or Dye SYBR Green
Passive Reference Dye ROX (Pre-mixed)
Product Line SYBR
Product Type Real Time PCR SYBR Master Mix
Purity or Quality Grade LD (Low DNA)
Quantity 1 x 1 mL
Shipping Condition Wet Ice
Sufficient For 40 Reactions of 50 μL
For Use With (Application) Gene Expression
No. of Reactions 40 Reactions
Sample Type DNA (Genomic), cDNA
Volume 1 x 1 mL
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For Research Use Only. Not for use in diagnostic procedures.