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Applied Biosystems™ TaqMan™ Gene Expression Assay (FAM) DFS Item

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5' end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3' end.

$138.00 - $1874.00

Specifications

Content And Storage 1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15°C to -25°C.

Detection Method Primer-probe
GC-Rich PCR Performance High
PCR Method qPCR
Reaction Speed Fast
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Products 6
Catalog Number Mfr. No. No. of Reactions Price Quantity  
Catalog Number Mfr. No. No. of Reactions Price Quantity  
4331182 DFS Item
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Applied Biosystems™
4331182
S (250 x 250 μL Reactions), inventoried
Each for $329.00
 
4351368 DFS Item
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Applied Biosystems™
4351368
L (2900 x 967 μL Reactions), made to order
Each for $1,874.00
 
4351370 DFS Item
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Applied Biosystems™
4351370
M (750 x 750 μL Reactions), made to order
Each for $864.00
 
4351372 DFS Item
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Applied Biosystems™
4351372
S (360 x 360 μL Reactions), made to order
Each for $510.00
 
4448892 DFS Item
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Applied Biosystems™
4448892
XS (75 x 75 μL Reactions), made to order
Each for $138.00
 
4453320 DFS Item
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Applied Biosystems™
4453320
XS (75 x 75 μL Reactions), inventoried
Each for $138.00
 
Description

Description

Applied Biosystems TaqMan Gene Expression assays are used for quantitative real-time PCR analysis of gene expression and consist of a pair of unlabeled PCR primers and a TaqMan probe with a dye label (FAM) on the 5’ end and a minor groove binder (MGB) and non-fluorescent quencher (NFQ) on the 3’ end.

Features of TaqMan Gene Expression assays include:
Easy to use—just add cDNA and master mix and run the qPCR—no melt curves required
Specific—TaqMan assays use proprietary MGB-containing probes that can be up to 15 bases shorter than non-MGB probes, improving the specificity of the assay
Sensitive—TaqMan assays are ideal for measuring low levels of expression or low-abundance targets
Accurate—identify small fold-changes with high accuracy of quantitation
Extensive content—Over 1.8 million predesigned assays available for over 25 different species
Gold-standard TaqMan qPCR chemistry—TaqMan assays draw on Thermo Fisher Scientific's bioinformatics assay design pipeline to help ensure high specificity and minimal cross-reactivity, even for gene variants with high sequence homology
Fast delivery—your assays shipped in as little as 1–2 days (see below)

Approximate ship time
Inventoried: 1–2 days in North America, 3–5 days in Europe*
Made to order: 4–6 days in North America, 6–10 days in Europe

TaqMan Gene Expression assays are the gold standard in real-time PCR gene expression studies, built on more than 20 years of experience. Each assay includes target primers and a sequence-specific probe optimized for the best functional performance. No additional design, optimization, or melt curve analysis is needed. Available in a wide variety of formats and species, new assay designs are constantly added to help meet your research needs. TaqMan assays have been cited in over 40,000 publications and are backed by more than 350 patents.

Pre-formulated assay
• 2 unlabeled PCR primers (900 nM each final 1X concentration)
• 1 FAM dye-labeled TaqMan MGB probe (250 nM final 1X concentration)

All of our predesigned TaqMan Gene Expression assays are covered by the TaqMan Assays qPCR Guarantee.**

Recommended master mix: TaqMan Fast Advanced Master Mix (Cat. No. 4444556)

*Our most popular assays are stocked in North America and Europe for overnight delivery where possible. If an assay is out of stock, it may take an additional 3–5 business days for delivery.

**Subject to terms and conditions. For complete details, go to www.thermofisher.com/taqmanguarantee.

Specifications

Specifications

1 tube containing 20X (XS, S, and M sizes) or 60X (L size) mix of pre-formulated assay (1 probe and 2 primers).

Store at -15°C to -25°C.

High
Fast
TaqMan™
Room Temperature
Primer-probe
qPCR
Sustainable packaging
Gene Expression Assay
60 X
SDS
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For Research Use Only. Not for use in diagnostic procedures.