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Substrates

Substrates are laboratory reagents, chemicals, and consumables, and kits used to support or enhance biomedical, pharmaceutical, clinical, food science, environmental, and other research and testing laboratory processes. Products include enzymes, inhibitors, acids, bases, buffers, antibodies, and chromatography supplies.
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1 – 15 of 80 results
1

Invitrogen™ Nitro blue Tetrazolium Chloride (NBT)  DFS Item

Use to assess cell viability as function of redox potential

3

Thermo Scientific™ LanthaScreen™ Tb-DUB Substrate  DFS Item

Deubiquitinating enzymes (DUBs) play housekeeping functions by maintaining pools of active ubiquitin, and regulatory function by rescuing specific target proteins from degradation by the proteasome.

4

Thermo Scientific™ GFP-4E-BP1  DFS Item

4E-BP1 expressed as a fusion with GFP (Green Fluorescent Protein) to act as a physiological substrate for FRAP1 (mTor).

6

Thermo Scientific™ Vivid™ EOMCC Substrate  DFS Item

Vivid™ Substrates are blocked dyes that yield a minimal fluorescence signal until oxidation occurs.

Shipping Condition Approved for shipment on Wet or Dry Ice
Substrate EOMCC
Substrate Properties Chemical Substrate
Content And Storage Vivid™ CYP450 Screening kits include:
• Vivid™ CYP450 Fluorogenic substrates (also available separately)
• BACULOSOMES™ CYP450 reagents
• Fluorescent standards
• NADPH-regeneration system

Store reaction buffers at +4°C or room temperature. Store BACULOSOMES™, the NADPH-regeneration system, and NADP+ at -80°C. Store the Vivid™ substrates and standards at +4°C, protected from light. (For long-term storage, resuspend in organic solvent and keep at -20°C.)
Substrate Type Cytochrome P450 Substrate
Product Line VIVID
Detection Method Fluorescence
7

Thermo Scientific™ CDK7/9tide Substrate  DFS Item

A peptide substrate based upon the C-terminal tail of RNA Polymerase II that is a reported substrate for Cyclin Dependent Kinases (CDK7, CDK8 and CDK9).

8

Thermo Scientific™ ZIPtide Substrate  DFS Item

A peptide substrate for the Ca/Calmodulin-dependent protein kinase (CaM) family including CAMK1 and DAPK1.

9

Thermo Scientific™ PIP2:PS Lipid Kinase Substrate  DFS Item

Specifically optimized for use with the Class I PI3 kinases (PIK3CA, PIK3CB, PIK3CD, and PIK3CG), this substrate consists of lipid vesicles of a 1:19 molar ratio of phosphoinositol-4,5-bisphosphate (PIP2) with phosphotidylserine (PS).

10

Thermo Scientific™ CHKtide  DFS Item

A peptide substrate based upon CDC25C that is phosphorylated by CHEK1 and other kinases.

11

Invitrogen™ MUP (4-Methylumbelliferyl Phosphate, Free Acid)  DFS Item

MUP is a fluorescent substrate for alkaline phosphatases

12

Invitrogen™ PFB-FDGlu (5-(Pentafluorobenzoylamino)Fluorescein Di-β-D-Glucopyranoside)  DFS Item

PFB-FDGlu is a cell-permeable substrate used for live cell imaging of GCase activity. It fluoresces green upon cleavage by lysosomal GCase, facilitating specific, single-cell analysis via high-content imaging, flow cytometry, and microscopy.

15

Invitrogen™ Rhodamine 110, bis-(p-Tosyl-L-Glycyl-L-Prolyl-L-Arginine Amide)  DFS Item

This Molecular Probes™ bisamide derivative of rhodamine 110 (R110) is a sensitive and selective substrate for assaying proteases in solution or inside living cells.