Cell Function Reagents and Kits
Invitrogen™ XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)
Redox-based viability probe
Invitrogen™ eBioscience™ Calcein Violet 450 AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Violet fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Violet 450.
Invitrogen™ eBioscience™ Calcein Blue AM Viability Dye
Membrane-permeable live-cell labeling dye. Upon entering the cell, intracellular esterases cleave the acetoxymethyl (AM) ester group, yielding the membrane-impermeable Calcein Blue fluorescent dye. Apoptotic and dead cells with compromised cell membranes do not retain Calcein Blue.
Invitrogen™ eBioscience™ Indo-1 AM Calcium Sensor Dye
Membrane-permeable dye used to determine changes in calcium concentrations in the cell using fluorescence microscopy, flow cytometry, fluorescence spectroscopy and fluorescence microplate readers. Once Indo-1 enters the cell, esterases cleave the AM group yielding a membrane-impermeable dye.
Invitrogen™ eBioscience™ Fura-2 AM Dye
Imaging dye that binds to free Ca2+. Fura-2 is the preferred dye for ratiometric imaging microscopy with digital image analysis, especially when altering excitation wavelengths is more practical than detecting multiple emission wavelengths.
Invitrogen™ eBioscience™ Calcium Sensor Dye eFluor™ 514
Membrane-permeable dye used to monitor changes in intracellular free calcium concentrations in the cell. Once eFluor™ 514 Calcium Sensor Dye enters the cell, cellular esterases cleave the AM group yielding a membrane-impermeable dye fluorescing at ≈520nm.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 450
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ eBioscience™ DRAQ5™
Anthraquinone dye with high affinity for double-stranded DNA. It is a membrane-permeable dye that can label live or fixed/dead cells.
Invitrogen™ Click-iT™ Plus EdU Alexa Fluor™ 350 Flow Cytometry Assay Kit
The Click-iT Plus EdU Alexa Fluor 350 Flow Cytometry Assay Kit provides a simplified, more robust assay for analyzing DNA replication in proliferating cells compared to traditional BrdU methods.
Invitrogen™ CellEvent™ Senescence Green Flow Cytometry Assay Kit
The CellEvent Senescence Green Flow Cytometry Assay Kit enables flow cytometric detection of cellular senescence via β-galactosidase hydrolysis.
Molecular Probes™ Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit
Click-iT™ Plus EdU Pacific Blue™ Flow Cytometry Assay Kit
Invitrogen™ CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
CellTrace™ Violet Cell Proliferation Kit, for flow cytometry
Molecular Probes™ LIVE/DEAD™ Cell Vitality Assay Kit, C12 Resazurin/SYTOX™ Green
The LIVE/DEAD Cell Vitality Assay Kit provides a simple, two-color fluorescence assay that distinguishes metabolically active cells from injured cells and dead cells.