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Invitrogen™ Amplex™ Red Glucose/Glucose Oxidase Assay Kit
Description
The Amplex Red Glucose/Glucose Oxidase Assay Kit provides a sensitive and simple method for detecting d-glucose or glucose oxidase using a fluorescence microplate reader or fluorometer. The Amplex Red reagent (10-acetyl-3,7-dihydroxyphenoxazine) is a colorless, stable, and extremely versatile peroxidase substrate. Because peroxidase- and glucose oxidase-mediated reactions can be coupled, it is possible to measure glucose oxidase activity or the release of glucose by any glucosidase enzyme—for instance, β-glucosidase and glucocerebrosidase—in either a continuous or discontinuous assay. Because of the ability to couple reactions, this assay has been demonstrated to be useful for the quantification of glucose levels in foods, fermentation media, and bodily fluids. In the assay, glucose oxidase reacts with d-glucose to form d-gluconolactone and hydrogen peroxide. In the presence of horseradish peroxidase (HRP), hydrogen peroxide then reacts with the Amplex Red reagent in a 1:1 stoichiometric ratio to generate the red-fluorescent product resorufin. Because resorufin has absorption and fluorescence emission maxima of approximately 571nm and 585nm, respectively, there is little interference from autofluorescence in most biological samples.
- Detect concentrations as low as 3μM d-glucose or 0.05mU/mL of glucose oxidase
- Format allows for multiple time point measurements
- Designed for minimal autofluorescence interference
- Custom assay design and packaging are also available
Use Amplex Red Assays for a Broad Range of Investigations
A wide variety of validated Amplex Red assays are available for studying cell signaling and lipids, neurobiology, inflammation and immune function, and metabolism. We also offer Amplex UltraRed Reagent, a second-generation reagent providing greater sensitivity and brighter fluorescence, and the Amplex Red/UltraRed Stop Reagent. The Amplex Red/UltraRed Stop Reagent provides convenience and control by allowing the fluorescence signal-generating reaction to be terminated at a user-determined time point. After addition of the stop reagent, the fluorescence signal remains stable for at least three hours.
Enzyme and Protein Activity Assays, Oxidase and Peroxidase Activity, Proteins, Expression, Isolation and Analysis
Order Info
Shipping Condition: Room temperature
Specifications
Specifications
| Content And Storage | Store in freezer -5°C to -30°C and protect from light. |
| Detection Method | Fluorescence |
| For Use With (Application) | Glucose/Glucose Oxidase Assay |
| For Use With (Equipment) | Microplate Reader, Spectrophotometer, Fluorometer |
| Product Type | Amplex Red Assay Kit |
| Dye Type | Other Label(s) or Dye(s) |
| Format | Tube(s), 96-well plate |
| Product Line | Amplex |
| Quantity | 500 Assays |
| Shipping Condition | Room Temperature |
Frequently Asked Questions (FAQs)
The components of Krebs-Ringer buffer (salts) should not cause oxidation of the Amplex reagent (which, in the presence of peroxidase and H2O2 oxidizes to resorufin, which is pink in color and fluorescent). Try water alone (the water used to make the Krebs-Ringer buffer). Since Hank's Buffered Saline Solution is typically purchased rather than made in the lab, it likely would not have the same contaminant. Another option is to degas the buffer prior to use to removed dissolved oxygen radicals.
This is not recommended. The presence of endogenous proteases can complicate the assay by degrading the horseradish peroxidase (HRP). Endogenous peroxidases and antioxidants can modify the H2O2 required for the reaction, competing with HRP (and catalase) for the substrate.
The Amplex Red Assays are best performed with either purified enzymes or extracted H2O2 in a defined buffer system, extracellular solutions or body fluids (media, serum, etc.) that do not exhibit high levels of endogenous protease or oxidase activity and do not contain antioxidants.
For Research Use Only. Not for use in diagnostic procedures.