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Redox-based viability probe
Monitor cell proliferation and viability with the resazurin-based PrestoBlue and PrestoBlue HS cell viability reagents. These nontoxic and cell permeable reagents quantitatively measure cell viability in as little as 10 minutes using absorbance- or fluorescence-based microplate readers.
Méthode de détection | Colorimetric, Fluorescence |
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À utiliser avec (équipement) | Microplate Reader,Spectrophotometer,HTS Reader |
Type de produit | Cell Viability Reagent |
Gamme de produits | PrestoBlue |
Forme | Liquid |
À utiliser avec (application) | Viability Assay |
Contenu et stockage | Store at 2°C to 8°C and protect from light. |
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
LIVE/DEAD™ BacLight™ Bacterial Viability Kits are easy-to-use fluorescent staining kits commonly used for assessing the viability of bacterial cells. These kits provide a two-color fluorescence assay of bacterial viability using green and red fluorescent dyes for use with imaging, flow, and assays.
Méthode de détection | Fluorescence |
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Type de produit | Bacterial Viability Kit |
Gamme de produits | BacLight, LIVE/DEAD |
Type de cellule | Bacteria |
Conditions d’expédition | Room Temperature |
Type de colorant | Other Label(s) or Dye(s) |
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
Type de réactif | Cell Tracker Compounds, Cell Labeling Reagents |
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À utiliser avec (équipement) | Fluorescence Microscope |
Conditions d’expédition | Room Temperature |
Type de colorant | Other Label(s) or Dye(s) |
À utiliser avec (application) | Cell Tracing,Cell Tracker |
Contenu et stockage | Store in freezer (-5°C to -30°C) and protect from light. |
Useful with a variety of fluorescence-detection methodologies
Detect and quantify ATP in biochemical and cell viability assays with the ATP Determination Kit. This luminescence-based ATP assay utilizes the luciferase-luciferin reaction, offering exceptional sensitivity to detect as little as 0.1 picomole of ATP using a luminometer or microplate reader.
Detectis galactose or monitors galactose oxidase activity
Sensitive, one-step assay to detect hydrogen peroxide (H2O2) or peroxidase activity
Format allows for multiple time point measurements
For use with absorbance and fluorescence intensity techniques
Format allows for multiple time point measurements
The Amplex Red and UltraRed reagents are fluorogenic substrates for peroxidase. These highly sensitive and stable probes react with H2O2 in the presence of HRP for solution-based assays and ELISAs. Amplex UltraRed is a brighter version of Amplex Red with an improved pH range and sensitivity.
Méthode de détection | Fluorescence |
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À utiliser avec (équipement) | Fluorometer,Microplate Reader |
Substrat | HRP (Horseradish Peroxidase) Substrate |
Type de produit | Red Hydrogen Peroxide |
Gamme de produits | Amplex |
Application validée | Cell Analysis |
Conditions d’expédition | Room Temperature |
Forme | Solid |
À utiliser avec (application) | Hydrogen Peroxidase Assay |
Contenu et stockage | Store in freezer (-5°C to -30°C). |
Detects uric acid or uricase using either a fluorescence microplate reader or fluorometer