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Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells DFS Item

Useful with a variety of fluorescence-detection methodologies

Supplier:  Invitrogen™ L3224

Catalog No. L3224



The LIVE/DEAD Viability/Cytotoxicity Kit is a quick and easy two-color assay to determine viability of cells in a population based on plasma membrane integrity and esterase activity. The kit can be used in flow cytometry, fluorescence microscopy, and with fluorescence microplate readers.

  • Faster, Safer, more sensitive
  • Less expensive
Easily Use with a Wide Variety of Techniques and Cell Types
Ubiquitous intracellular esterase activity and an intact plasma membrane are distinguishing characteristics of live cells. The LIVE/DEAD Viability/Cytotoxicity Kit quickly discriminates live from dead cells by simultaneously staining with green-fluorescent calcein-AM to indicate intracellular esterase activity and red-fluorescent ethidium homodimer-1 to indicate loss of plasma membrane integrity. It is adaptable to most eukaryotic cells where cytotoxic conditions produce these cellular effects. The assay is useful with a variety of fluorescence-detection methodologies.

Sensitive, Safe, and Efficient
The LIVE/DEAD Viability/Cytotoxicity Kit is more sensitive than Trypan blue exclusion, a commonly used method for live/dead cell discrimination. The cost-effective LIVE/DEAD Viability/Cytotoxicity Kit is highly sensitive due to the bright fluorescence of both dyes upon interacting with either live (for calcein-AM) or dead (for ethidium homodimer-1) cells. Background levels are low due to the fact that both dyes are virtually non-fluorescent prior to interacting with cells.

LIVE/DEAD Assays Available for a Broad Range of Applications
A selection of Invitrogen LIVE/DEAD Viability Assays is offered for mammalian cells, bacteria, yeast and fungi, as well as Fixable Dead Cell Stain Kits for use in intracellular staining for flow cytometry. All LIVE/DEAD assays provide quick, positive discrimination between viable and non-viable cells.

Cell Analysis, Cell Counting and Viability, Cell Counting, Viability, and Cryopreservation, Cell Culture, Cell Metabolism, Cell Viability and Cytotoxicity, Cell Viability, Proliferation and Function, Cellular Imaging, Cellular Toxicology Assays, Drug Discovery and Development, Flow Cytometry, Flow Cytometry Viability and Cytotoxicity Assays, Flow Cytometry of Cellular Processes, High-Content Screening (HCS), Immunofluorescence (IF), Immunofluorescence Staining and Detection, Target-Based ADME/Tox Assays

Order Info

Shipping Condition: Room temperature



Mammalian Cells
Store in freezer at -5°C to -30°C and protect from light.
Fluorescence Microscope, Flow Cytometer, Microplate Reader
Other Label(s) or Dye(s)
494, 528 nm
Green, Red
LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Viability Assay
Viability/Cytotoxicity Kit
517, 617 nm
Tube(s), 96-well plate, Slide(s)
Room Temperature




For Research Use Only. Not for use in diagnostic procedures.