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Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780

A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.

$173.00 - $590.00

Specifications

Color Infrared
Detection Method Fluorescent
For Use With (Application) Viability Assay
For Use With (Equipment) Flow Cytometer
Product Type Dye
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Products 2
Catalog Number Mfr. No. Quantity Price Quantity  
Catalog Number Mfr. No. Quantity Price Quantity  
501129035
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Invitrogen™
65086514
100 Tests
Each for $173.00
 
5016966
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Invitrogen™
65086518
500 Tests
Each for $590.00
 
Description

Description

  • Unlike 7-AAD and propidium iodide, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized, and stained for intracellular antigens without any loss of staining intensity of the dead cells
  • Thus, using Fixable Viability Dyes allows dead cells to be excluded from analysis when intracellular targets are being studied
  • Fixable Viability Dye eFluor™ 780 can be excited by the red (633 nm) laser line and has a peak emission of 780 nm that can be detected using a 780/60 band pass filter (equivalent to APC-eFluor™ 780 or APC-Alexa Fluor™ 750)
  • For compensation, it is recommended to use a sample of the cells of interest stained with the Fixable Viability Dye
  • If the percentage of dead cells is expected to be less than 5%, then it is recommended to take a small aliquot of cells and heat them at 65°C for 1 minute then immediately place on ice for 1 minute
  • After this treatment, the heat-killed cells can be combined 1:1 with live cells and then stained with the Fixable Viability Dye
  • Testing suggests that compensation out of most detectors is negligible, with compensation out of PE-Cyanine7 being the highest at less than 5%
  • Actual compensation values will depend on each investigator's specific instrument, filter sets, and PMT voltage settings
  • Fixable Viability Dye eFluor™ 780 is supplied as a pre-diluted solution prepared in high-quality, anhydrous DMSO
  • It should be protected from light and moisture
  • Store at -70°C with dessicant, it may be freeze-thawed up to 20 times
  • Allow vial to equilibrate to room temperature before opening
Specifications

Specifications

Infrared
Viability Assay
Dye
eBioscience™
Fluorescent
Flow Cytometer
633/780
SDS
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For Research Use Only. Not for use in diagnostic procedures.