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Gas chromatography (GC) is used to separate chemicals based on their volatility, or the ease with which they evaporate into a gas. Samples are injected through a heated injection port into an inert carrier gas such as helium, argon or nitrogen. This vaporized mobile phase moves through either a packed column with a solid support or a capillary column with a liquid (high-boiling polymer) stationary phase; columns are housed in a temperature-controlled oven to facilitate movement of the molecules. Sample components are separated based on interactions with the stationary phase as they pass through the column.

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High-performance liquid chromatography (HPLC) is a technique used to separate a mixture of compounds with the purpose of identifying and analyzing the components. The mixture is injected into a polar solvent, or eluent; this mobile phase is then pumped at high pressure through a column. The column, or stationary phase, is packed with nonpolar or hydrophobic silica particles bonded with alkyl chains. As the mobile phase passes through the column, the differences in travel rate, or retention time, of the components facilitates separation. Components are detected by ultraviolet absorption; the absorption spectrum is captured as a chromatogram, which is then used for analysis.

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Sample Prep

Sample preparation is a key component in driving consistent separations, sample protection and ensuring long column and instrument life. Sample prep techniques can be as simple as chromatography specific syringe filters all the way to solid phase extraction, depending on your analysis needs.

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