Chromatography

The term chromatography describes a variety of techniques that are used to separate a substance or mixture into its components. Many products are used during different stages of chromatography workflows, including solvents, standards, columns, vials and closures, and chromatography paper.

Chromatography can be preparative or analytical. It can be used to prepare a substance for analysis or used to measure the amount or proportion of specific components in the substance.

The choice of chromatography method depends on the specific application and substance, as well as the polarity of the separation media and solvent. Commonly used chromatography methods include:

  • Liquid chromatography
    • High performance liquid chromatography (HPLC): One of the most frequently used research and analytical techniques; great for small molecule analysis 
    • Flash chromatography: Ideal for separating large sample volumes
    • Fast protein liquid chromatography (FPLC): Uses ion exchange to separate large biomolecules
    • Thin layer chromatography: Silica coated glass or alumina plates are dotted with a substance; one end of the plate is immersed in solvent, which carries and separates the components as it travels
  • Gas chromatography
    • Samples are heated to a gaseous state
    • Well-suited for petrochemical, environmental, and industrial chemical research and testing
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The Fisher Scientific channel offers a full range of chromatography products to suit your specific needs. We cover each step of your workflow, providing everything from vials and closures, solvents, and standards to liquid and gas chromatography columns and thin layer chromatography paper — all from trusted suppliers and brands.

The term chromatography describes a variety of techniques that are used to separate a substance or mixture into its components. It is based on the principle that the weight or size of the component will affect the speed at which it travels through another medium. When the components move at different rates, they naturally create a separation.

Chromatography can be both preparative and analytical. It can be used to process a substance before it is analyzed or used as an analytic method itself to measure the amount or proportion of a component in the substance.

The choice of chromatography methods depends on the specific application and substance, as well as the polarity of the media and solvent. Commonly used chromatography methods include:

  • Paper chromatography
    • Paper is dotted with a substance
    • One end of the paper is immersed in solvent, which carries the components with it as it travels through the paper
  • Thin layer chromatography (TLC)
    • Similar to paper chromatography, but uses a glass plate coated with adsorbent as a medium
    • Used for drug levels and water purity testing
  • Gas chromatography
    • Requires that the sample be heated to a gaseous state
    • Well-suited for petrochemical, environmental, and industrial chemical research and testing
  • Liquid chromatography
    • Includes High Performance Liquid Chromatography (HPLC), one of the most frequently used research and analytical techniques
  More
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Liquid Chromatography

Liquid chromatography columns for HPLC, UHPLC, FPLC, and LC-MS are available for small and large molecule (biomolecule) analysis. They come in reversed phase (C18, C8, RP-amide polar-embedded, phenyl, diol, cyano), normal phase, ionic, size exclusion, and preparatory column varieties. For optimal LC system performance, consider high-quality solvents and buffers, LC calibration standards, autosampler vials, solid phase extraction cartridges, and other LC accessories.

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Gas Chromatography

GC and GC/MS capillary gas chromatography columns serve multiple industries, including environmental, petroleum, chemical, food and beverage, and pharmaceutical. Packed GC columns and components are also availble. To maximize performance, choose compatible GC accessories, including GC inlet liners, septa, syringes, vials, calibration standards, extraction solvents, and gas management products (including gas generators and pressure regulators).

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Complete Your Workflow

Find other products for your full chromatography workflow.

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Chromatography Accessories

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