Life Science Buffers

Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
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Filtered Search Results

HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent.
Molecular Weight (g/mol) | 238.30 |
---|---|
ChEBI | CHEBI:42334 |
Color | White |
Chemical Name or Material | HEPES |
Grade | Molecular Biology |
SMILES | OCCN1CCN(CCS(O)(=O)=O)CC1 |
Identification | Pass Test |
DNase | DNase free |
Merck Index | 15, 4689 |
InChI Key | JKMHFZQWWAIEOD-UHFFFAOYSA-N |
ChemAlert Storage Symbol | Gray |
Assay Percent Range | ≥99 % |
PubChem CID | 23831 |
Absorbance | 0.01 max. (0.1M solution) at 280nm |
Percent Purity | ≥99% |
CAS | 7365-45-9 |
Protease | Protease free |
Health Hazard 3 | Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1 |
MDL Number | MFCD00006158 |
Health Hazard 2 | WARNING! |
Solubility Information | Soluble in water |
pH | 5.0 to 6.5 |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |
Molecular Formula | C8H18N2O4S |
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Content And Storage | Room temperature |
---|---|
Form | Liquid |
Packaging Type | Poly Cube |
pH | 7.3 to 7.5 |
Melting Point | 0°C |
Boiling Point | 100°C |
---|---|
Name Note | 1X Solution, pH 7.4 |
CAS | 77-86-1 |
Protease | Protease free |
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
Synonym | TBS |
DNase | DNase free |
Recommended Storage | RT |
ChemAlert Storage Symbol | Gray |
Gibco™ PBS, pH 7.4
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PBS (phosphate buffered saline) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Shipping Condition | Room Temperature |
---|---|
Form | Liquid |
Color | Clear |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Washing Cells Before Dissociation; Diluting Cells for Counting and Preparing Reagents; Transporting Cells or Tissue |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 280 - 315 mOsm/kg |
Dilution | 1 X |
Additive | No Sodium Pyruvate |
Product Type | Phosphate Buffered Saline (PBS) |
Classification | Animal Origin-Free |
pH | 7.4 |
Solution Type | Phosphate Buffered Saline |
Product Line | Gibco™ |
Gibco™ DPBS, calcium, magnesium
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Dulbecco's phosphate-buffered saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents.
Dilution | 1 X |
---|---|
Additive | No Sodium Pyruvate |
Form | Liquid |
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.2 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Washing Cells Before Dissociation, Transporting Cells or Tissue Samples, Diluting Cells for Counting, and Preparing Reagents |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Product Line | Gibco™ |
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 8.0, 0.2 micron filtered
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00004679 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Thermo Scientific™ TRIS, 1.0M buffer soln., pH 8.0
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
MDL Number | MFCD00004679 |
SMILES | C(C(CO)(CO)N)O |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Thermo Scientific Chemicals Potassium phosphate, 0.5M buffer soln., pH 7.4
CAS: 7778-77-0 Molecular Formula: H2KO4P Molecular Weight (g/mol): 136.08 MDL Number: MFCD00011401 MFCD00147253 InChI Key: GNSKLFRGEWLPPA-UHFFFAOYSA-M IUPAC Name: potassium dihydrogen phosphate SMILES: [K+].OP(O)([O-])=O
CAS | 7778-77-0 |
---|---|
Molecular Weight (g/mol) | 136.08 |
MDL Number | MFCD00011401 MFCD00147253 |
SMILES | [K+].OP(O)([O-])=O |
IUPAC Name | potassium dihydrogen phosphate |
InChI Key | GNSKLFRGEWLPPA-UHFFFAOYSA-M |
Molecular Formula | H2KO4P |
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
CAS | 10043-35-3 |
---|---|
Synonym | TBE |
TRIS, 0.1 Molar In Dilute Hydrochloric Acid, Reagents
CAS: 7732-18-5 Molecular Formula: H2O Molecular Weight (g/mol): 18.02 InChI Key: XLYOFNOQVPJJNP-UHFFFAOYSA-N Synonym: Tromethamine IUPAC Name: water SMILES: O
CAS | 7732-18-5 |
---|---|
Molecular Weight (g/mol) | 18.02 |
SMILES | O |
Synonym | Tromethamine |
IUPAC Name | water |
InChI Key | XLYOFNOQVPJJNP-UHFFFAOYSA-N |
Molecular Formula | H2O |
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
PubChem CID | 6503 |
---|---|
CAS | 77-86-1 |
Molecular Weight (g/mol) | 121.136 |
ChEBI | CHEBI:9754 |
SMILES | C(C(CO)(CO)N)O |
Synonym | Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol |
IUPAC Name | 2-amino-2-(hydroxymethyl)propane-1,3-diol |
InChI Key | LENZDBCJOHFCAS-UHFFFAOYSA-N |
Molecular Formula | C4H11NO3 |
Gibco™ DPBS, no calcium, no magnesium
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Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Form | Liquid |
---|---|
Product Type | DPBS |
Classification | Animal Origin-Free |
pH | 7.0 to 7.3 |
Manufacturing Quality | cGMP-compliant under the ISO 13485 standard |
For Use With (Application) | Mammalian Cell Culture |
Sterility | Sterile-filtered |
Phenol Red Indicator | No Phenol Red |
Osmolality | 270 - 300 mOsm/kg |
Solution Type | Dulbecco's Phosphate Buffered Saline |
Gibco™ HEPES (1M)
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A zwitterionic organic chemical buffering agent commonly used in cell culture media
Sterilization Method | Sterile-filtered |
---|---|
Physical Form | Liquid |
Chemical Name or Material | Zwitterionic Organic Chemical Buffer |
Grade | Biochemical |
For Use With (Application) | Chromatin Biology |
Sterility | Sterile |
Solution Type | Buffer |
Boiling Point | 100°C |
---|---|
Color | Colorless |
Physical Form | Liquid |
Chemical Name or Material | Tris Buffered Saline |
Grade | Molecular Biology |
DNase | DNase free |
ChemAlert Storage Symbol | Gray |
Name Note | 10X Solution |
Concentration or Composition (by Analyte or Components) | 10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl. |
CAS | 7732-18-5 |
Protease | Protease free |
pH | 7.5 |
Synonym | TBS |
Purity Grade Notes | DNase-, RNase- and Protease-Free |
Recommended Storage | RT |