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Protein Purification

Kits, columns, gels, reagents, enzymes, inhibitors, and other assorted products for use with protein purification procedures.
Protein Purification Reagents and Kits (671)
Protease Inhibitors and Phosphatase Inhibitors (280)
Magnetic Affinity Separation Particles (123)
Gel Filtration Media and Columns (85)
Spin Columns and Gravity-flow Columns (53)
Cell Lysis Detergents (49)
In vitro Protein Expression Systems (46)
Miscellaneous Immobilized Affinity Ligands (45)
Biotin-Binding Affinity Resins and Beads (34)
Miscellaneous Immobilization Supports (28)
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Cell Lysis Enzymes (23)

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1

Gibco™ ExpiSf™ Protein Production Kit

Gibco™ ExpiSf™ Protein Production Kit is designed to significantly boost protein expression from high-density suspension cultures of Sf9 insect cells using the ExpiSf Expression System.

Shipping Condition Approved for shipment at Room Temperature or on Wet Ice
Expression Mechanism Cell-Based Expression
Format Liquid
Compatible Cells Insect Cells
Classification Animal Origin-Free, Chemically Defined, Protein-Free, Serum-Free
Expression System Baculovirus
Product Line ExpiSf
2

Thermo Scientific™ Pierce™ Chromogenic Endotoxin Quant Kit

Chromogenic Endotoxin Quant Kits are highly sensitive endpoint assays that measure and detect endotoxin (LPS) in protein, peptide, nucleic acid, or antibody samples using the Amebocyte Lysate Assay from 0.01–1.0 EU/mL. Kits are available in reaction sizes ranging from 30 to 240.

Shipping Condition Wet Ice
Assay End-point Chromogenic Assay
Type Endotoxin Quantitation Kit
Target Endotoxins
Content And Storage • Lyophilized amebocyte lysate
• E.coli endotoxin standard
• Chromogenic substrate
• Endotoxin-free water

Store at 2–8°C upon receipt.
Format Kit
Assay Sensitivity 0.01 EU/mL
Assay Range 0.01 to 0.1 EU/mL, 0.1 to 1.0 EU/mL
Product Line Pierce
Detection Method Colorimetric
3

Thermo Scientific™ Pierce™ Streptavidin Magnetic Beads

Purify or immunoprecipitate biotinylated proteins or affinity ligands using streptavidin-coated magnetic particles for magnet-based separation.

Particle Size 1 μm
Ligand Type Streptavidin
Type Magnetic Affinity Bead
Target Biotin
Content And Storage Upon receipt store at 4°C. Product is shipped on an ice pack.
Protein Form Recombinant
Form Liquid
Concentration Slurry: 10 mg/mL, 1% solids
Product Line Pierce
4

Invitrogen™ DynaMag™-2 Magnet  DFS Item

The DynaMag-2 magnet combines a strong magnetic attraction for efficient separations in standard microcentrifuge tubes with a flexible and smart ergonomic design.

5

Thermo Scientific™ Brij™-35, 30% Solution

Stabilized Brij-35 detergent provided as 10% and 30% solutions for use in various protein methods including cell lysis and HPLC.

6

Cytiva Sephadex™ LH-20

Use for molecular sizing of natural product such as steroid, terpenoid, lipid, low molecular weight peptide. Cytiva Life Sciences™Sephadex™ LH-20 is a liquid chromatography media useful to analytical and industrial scale for preparation of closely related molecular species.

Particle Size 18 to 111 μm in dry form
Height (Metric) 30cm
Flow Rate 720 cm/hr.
Content And Storage 4 to 30°C
Format Solid Spherical Bead
pH Range 2 to 13
Product Type Sephadex LH-20 Media
Sample Type Steroid, Terpenoid, Lipid, low molecular weight Peptide
Diameter (Metric) Inner 2.6cm
For Use With (Application) For fractionation of lipids, hormones, vitamins, and other small biomolecules in polar organic solvents and aqueous solvent mixtures
Support Type Hydroxypropylated, cross-linked Dextran
Max. Pressure 25-45 cm/h, pressure drop cm H2O/bed height=2, beh height 30 cm, 2.6 cm i.d.
Height (English) 11.81 in.
7

MilliporeSigma™ Calbiochem™ PP2

PP2, CAS 172889-27-9, is a potent, reversible, ATP-competitive, inhibitor of the Src family of protein tyrosine kinases (IC50 = 4, 5, 5, & 100 nM for p56lck, p59fynT, Hck, & Src, respectively).

CAS 172889-27-9
Form Solid
Target Proteins p56lck
Solubility DMSO
Color Off-white
Purity 95% by HPLC
Molecular Formula C15H16ClN5
Formula Weight 301.8g/mol
8

MilliporeSigma™ Calbiochem™ AEBSF, Hydrochloride

AEBSF, CAS 30827-99-7, is a specific irreversible inhibitor of chymotrypsin, kallikrein, plasmin, thrombin, trypsin, and related thrombolytic enzymes. A nontoxic alternative to PMSF and DFP.

Content And Storage Protect from moisture
CAS 30827-99-7
Form Solid
Target Proteins chymotrypsin
Solubility H2O
Color White to Off-white
Purity >97% by HPLC
Molecular Formula C8H10FNO2S·HCl
Formula Weight 239.7g/mol
9

MilliporeSigma™ Calbiochem™ Glyburide

A sulfonylurea that selectively blocks ATP-sensitive K+ channels.

10

MilliporeSigma™ Calbiochem™ Temozolomide

A cell-permeable, orally bioavailable, blood-brain barrier-permeant MTIC [5-(3-methyltriazen-1-yl-)-imidazole-4-carboxamide] prodrug whose in vivo DNA alkylating activity is employed in anticancer ther

11

MilliporeSigma™ Calbiochem™ 2-APB

A cell-permeable modulator of Ins(1,4,5)P3-induced Ca2+ release.

13

MilliporeSigma™ Calbiochem™ D,L-Sulforaphane

An isothiocyanate isolated from broccoli that acts as a potent inducer of phase II detoxifying enzymes in mouse tissues and murine hepatoma cells in culture.

14

MilliporeSigma™ Calbiochem™ GSK-3 Inhibitor XVI

GSK-3 Inhibitor XVI - CAS 252917-06-9, is a cell-permeable, potent, ATP-competitive, and highly selective GSK-3 inhibitor (IC50 = 10 and 6.7 nM against GSK-3α and GSK-3β, respectively).

15

MilliporeSigma™ Calbiochem™ Berberine Chloride

A highly potent and selective oxysterol EBI2 (GPR183) agonist (K