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HPLC Columns and Cartridges

Pre-packed columns, cartridges, and guard columns for separation of biomolecules and organic/inorganic analytes; includes normal/reversed phase HPLC, UHPLC, LC-MS, HILIC, mixed-mode, ion exchange, ligand exchange, and affinity column formats.
HPLC Columns (3,969)
HPLC Guard Columns and Cartridges (548)
HPLC Guard Column Accessories (33)

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1 – 15 of 1,266 results
1

Thermo Scientific™ Hypersil™ ODS C18 Columns

Use as a general purpose C18 phase for acidic and neutral analytes.

Pore Size 120 Å
Carbon Load 10%
Stationary Phase C18
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
pH 2 to 8
Surface Area 170 m²/g
USP Type L1
2

Thermo Scientific™ Hypersil GOLD™ aQ Javelin™ Guard HPLC Columns

Javelin guard columns offer convenient, easy to use protection for analytical HPLC columns

3

MilliporeSigma™ Supelco™ Ascentis™ Express C18 Micron Capillary HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
4

MilliporeSigma™ Supelco™ LiChrosorb™ RP-18 HPLC Column, 5 μm Particle Size

LiChrosorb™ RP-18 HPLC Columns are classified as L1 USP columns and are primarily used for reversed-phase HPLC of neutral, acidic and weak basic compounds. The totally porous irregular particles ensure reliable results.

Pore Size 100 Å
Particle Size 5 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/Silica Particle Platform
Product Type HPLC Column
Endcapped No
Surface Area 300 m2/g
Product Line LiChrosorb
5

MilliporeSigma™ Supelco™ Ascentis™ Express OH5 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express OH5 columns can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Pore Size 90 Å
Particle Size 2.7 μm
Stationary Phase Hydroxy, Penta-
Phase Reversed Phase
Matrix Hydroxy, Penta- Phase/Fused-Core Particle Platform
Endcapped No
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
6

MilliporeSigma™ Supelco™ Discovery™ BIO Wide Pore C18 HPLC Column, 3 μm Particle Size

The Discovery™ BIO Wide Pore C18 column has the ability to analyze peptides without TFA in the mobile phase, thereby increasing the LC-MS signal.

Pore Size 300 Å
Particle Size 3 μm
Stationary Phase C18 (octadecyl)
Phase Reversed Phase
Matrix C18 (octadecyl) Phase/silica particle platform
Endcapped Yes
Technique Wide Pore HPLC
Surface Area 100 m2/g
Product Line Discovery
7

MilliporeSigma™ Supelco™ Ascentis™ Express F5 HPLC Column, 5 μm Particle Size

Ascentis™ Express 5 μm F5 can be used for basic, acidic, or neutral compounds with alternate selectivity from C18.

Pore Size 90 Å
Particle Size 5 μm
Stationary Phase PFP (Pentafluorophenyl)
Phase Reversed Phase
Matrix PFP (Pentafluorophenyl) Phase/Fused-Core Particle Platform
Endcapped Yes
pH 1 to 9
Technique Fused-Core HPLC
Product Line Ascentis
8

Thermo Scientific™ MAbPac™ HIC-Butyl HPLC Columns
GREENER_CHOICE

Separate monoclonal antibodies (mAbs) and antibody-drug conjugates (ADCs) with excellent biocompatibility, low carryover and high resolution.

Pore Size Nonporous
Particle Size 5 μm
Temperature 60°C
Phase Hydrophobic Interaction
Packing Material Hydrophilic Polymer
Column Type Hydrophobic Interaction
pH 2 to 12
Quantity Each
Green Features FSC
Max. Pressure 4000 psi (275 bar)
Product Line MAbPac
9

Thermo Scientific™ Hypersil GOLD™ Cyano HPLC Columns

Alternate selectivity with lower hydrophobicity. Use these HPLC columns for normal and reversed phase separations.

Pore Size 175 Å
Carbon Load 4%
Stationary Phase Cyano (CN)
Temperature 60°C
Phase Normal and Reversed Phase
Endcapped Yes
pH 2 to 8
Surface Area 220 m²/g
USP Type L10
10

MilliporeSigma™ Supelco™ Ascentis™ Express 90 Å PAH, 2.7 μm HPLC Guard Cartridge

The unendcapped trifunctional C18 stationary phase of Ascentis™ Express PAH provides a stable, reversed-phase packing that can be used for analysis of acids, bases, and zwitterionic compounds, but has been specifically designed for the separation of polycyclic aromatic hydrocarbons.

Pore Size 90 Å
Particle Size 2.7 μm
Temperature <60°C
Matrix Fused-Core™ Silica Particle Platform Particle Platform Superficially Porous Particle
Length (Metric) 0.5 cm
Column Type Reversed Phase
pH 2 to 9
Quantity 3/Pk.
Material Stainless Steel
Surface Area 135 m2/g
Max. Pressure 600 bar
11

Thermo Scientific™ POROS™ CaptureSelect™ IgM Affinty HPLC Columns

Analytical groups supporting biologic drug development require rapid, sensitive and precise methods for protein quantitation from cell culture, fermentation harvests, or purification intermediates.

Shipping Condition Wet Ice
Pore Size 2000 Å
Stationary Phase IgM affinity ligand, human-, mouse-, and rat-specific
Column Type Affinity
Quantity 1 Column
Material PEEK
For Use With (Application) HPLC
High-throughput Compatibility Not High-throughput Compatible (Manual)
Max. Pressure 180 bar
Particle Size 20 μm
Flow Rate Up to 5000 cm/hr.
Matrix Polystyrenedivinylbenzene
Reactivity Human, Mouse, Rat
Target Molecule IgM Antibodies
Product Type Column
Binding Capacity 4 mg/mL
Sample Type Cell Culture Supernatants
Purification Target IgM Antibodies
Product Line CaptureSelect, POROS
12

Thermo Scientific™ HyperREZ™ XP Carbohydrate Pb2+ HPLC Columns

HyperREZ XP Carbohydrate Pb2+ LC Columns are used for the appropriate counter-ion to meet application requirements.

Particle Size 8 μm
Stationary Phase Pb2+ Counter Ion
Temperature 90°C
Phase Ligand Exchange
For Use With LC/MS
Packing Material Spherical, Microporous Polymer
pH 1 to 11
Max. Pressure 725 psi (50 bar)
USP Type L34
13

Thermo Scientific™ POROS™ CaptureSelect™ GCSF Affinty HPLC Columns

Thermo Scientific™ POROS™ CaptureSelect™ GCSF HPLC columns incorporate the advantages of high-speed POROS chromatography resins with the unique selectivity of CaptureSelect ligands.

Pore Size 2000 Å
Stationary Phase Granulocyte ColonyStimulating Factor affinity ligand, human-specific
Column Type Affinity
Elution Volume Max 29 column volumes
Quantity 1 Column
Material PEEK
For Use With (Application) FPLC
High-throughput Compatibility Automated Protocols
Max. Pressure 2600 psi, 180 bar
Particle Size 20 μm
Matrix POROS
Target Molecule Protein
Product Type Column
Binding Capacity 5.2 mg/mL (ligand density)
Purification Target Protein
Elution Volume Min 14 column volumes
Product Line CaptureSelect, POROS
14

MilliporeSigma™ Supelco™ Ascentis™ Express C18 HPLC Column, 2.7 μm Particle Size

Ascentis™ Express HPLC columns, through the use of Fused-Core™ particle technology, can provide you with both the high speed and high efficiencies of sub-2 μm particles while maintaining lower back pressures.

Pore Size 90 Å
Stationary Phase C18 (octadecyl)
Temperature 60°C
Phase Reversed Phase
Endcapped Yes
Column Type Reversed Phase
Material Stainless Steel
Max. Pressure 600 bar(9000 psi)
Column Format Analytical Column
Particle Size 2.7 μm
Matrix C18 (octadecyl) Phase/Fused-Core Particle Platform
Product Type HPLC/UHPLC Column
Packing Material Fused-Core particle platform superficially porous particle
pH 2 to 9
Technique Fused-Core HPLC
Product Line Ascentis
USP Type USP L1
15

Thermo Scientific™ HyPURITY™ C8 HPLC Columns, 5μm Particle Size

Thermo ScientificTM HyPURITYTM C8 HPLC Columns are available in limited dimensions. Please contact Customer Service for more details.

Pore Size 190 Å
Particle Size 5 μm
Carbon Load 8%
Stationary Phase C8
Phase Reversed Phase
Endcapped Yes
Packing Material Silica
Column Type Reversed Phase
pH 2 to 9
Surface Area 200 m²/g
USP Type L7