
Real Time PCR Reagents and Kits
Products designed for use during real-time (quantitative) PCR procedures. Includes master mixes, DNA polymerases, reverse transcriptases, size standards, kits, and reagents.
Reverse transcription polymerase chain reaction (RT-PCR) is a laboratory technique that combines the reverse transcription of RNA into DNA and the subsequent amplification of the DNA using PCR.
In RT-PCR, a reverse transcriptase enzyme converts an RNA template to its complementary DNA (cDNA). The cDNA then becomes the template for PCR amplification.
A typical RT-PCR kit includes the main components needed for the assay:
- Buffer
- Primers – a combination of deoxynucleotide triphosphates (dNTPs) of the four bases of adenine (dATP), cytosine (dCTP), guanine (dGTP), and thymine (dTTP)
- Reverse transcriptase reagent
The combination of RT-PCR and qPCR techniques (sometimes labelled RT-qPCR) is often used in research and clinical labs to analyze gene expression and quantify viral RNA. Since this method uses fluorescence to track the amplification reaction, these kits may also include:
- Fluorogenic primers or labeled fluorogenic probes
- Fluorescent dye
Reagents for cDNA PCR can be purchased separately or as cDNA synthesis kits.
RT-PCR is commonly used for RNA detection, molecular cloning, and gene sequencing. RT-qPCR is used for gene expression analysis, RNAi or microarray validation, pathogen detection, genetic testing, and other disease research.
- (49)
- (1)
- (10)
- (9)
- (69)
- (6)
- (1)
- (99)
- (17)
- (3)
- (3)
- (3)
- (3)
- (26)
- (4)
- (3)
- (21)
- (6)
- (21)
- (18)
- (5)
- (1)
- (1)
- (15)
- (15)
- (46)
- (5)
- (2)
- (32)
- (10)
- (2)
- (2)
- (5)
- (1)
- (1)
- (6)

Applied Biosystemsâ„¢ MycoSEQâ„¢ Plus Mycoplasma Detection Kits
The MycoSEQâ„¢ Plus Mycoplasma Detection Kit is a TaqManâ„¢-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
Shipping Condition | Dry Ice |
---|---|
GC-Rich PCR Performance | Medium |
No. of Reactions | 100 Reactions |
Product Type | Mycoplasma Detection |
Form | Liquid |
Sample Type | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
Reaction Speed | Standard |
Label or Dye | FAM |
For Use With (Application) | Microbial Detection |
PCR Method | Multiplex qPCR |
Target Organism Class | Mollicutes, mycoplasma |
Product Line | MycoSEQâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ SuperScriptâ„¢ III Platinumâ„¢ One-Step qRT-PCR Kit
Combine the most powerful reverse transcriptase and DNA polymerase technologies to deliver precise and accurate analysis of gene expression in a convenient one-step format.
Shipping Condition | Dry Ice |
---|---|
Polymerase | Taq DNA Polymerase |
Product Type | One-Step qRT-PCR Kit |
For Use With (Equipment) | 7500 System, BioRad iCycler iQ, BioRad iQ5, Stratagene Mx4000, MJ Chromo4, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Cepheid SmartCycler, BioRad MyiQ |
Sample Type | RNA |
Reaction Speed | Standard |
For Use With (Application) | Gene Expression,Pathogen Detection,Virus Detection |
PCR Method | 1-step RT-qPCR |
Product Line | Platinumâ„¢, SuperScriptâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ EXPRESS One-Step Superscriptâ„¢ qRT-PCR Kit, universal
Kit incorporates proven Invitrogenâ„¢ enzymes specifically tailored to deliver superior results on high-throughput, fast-cycling instruments for probe-based qPCR experiments.
Polymerase | Taq DNA Polymerase |
---|---|
Format | Tube |
Product Type | qRT-PCR Kit |
For Use With (Equipment) | 7500 Fast System, 7500 System,Applied Biosystems StepOnePlusâ„¢ Fast Real-Time PCR System, BioRad CFX96â„¢, StepOnePlusâ„¢, Fast Mode, StepOnePlusâ„¢, Standard Mode, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad MyiQ, Corbett RotorGene, Cepheid SmartCycler, MJ Opticon, MJ Chromo4, BioRad iCycler iQ |
Sample Type | RNA |
Reaction Speed | Fast or Standard |
PCR Method | 1-step RT-qPCR |
Product Line | SuperScriptâ„¢ |
Detection Method | Primer-probe |
Invitrogenâ„¢ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
Content And Storage | Store in freezer (-5° to -30°C). |
---|---|
Label or Dye | ROX |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ MycoSEQâ„¢ Mycoplasma Detection Kit, with PrepSeqâ„¢ sample preparation, 100 Reactions
The MycoSEQ Mycoplasma Detection Kit is a real-time mycoplasma PCR assay designed and validated to meet the requirements of European Pharmacopeia Chapter 2.6.7 for use as a nucleic acid amplification technique (NAT).
Applied Biosystemsâ„¢ ViralSEQâ„¢ Lentivirus Physical Titer Kits
A one-step RT-qPCR assay for the quantitation of genome-containing lentiviral particles in supernatants from cell-based bioproduction systems. Lentiviral quantitation is a critical quality attribute (CQA) in viral vector manufacturing for cell and gene-modified cell therapy development.
Target | Lentivirus genomic target |
---|---|
For Use With (Equipment) | Real-time PCR instruments (QuantStudio5 or 7500 Fast) |
Quantity | 100 reactions |
For Use With (Application) | Virus Detection |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ resDNASEQâ„¢ Quantitative Sf9 and Baculovirus DNA Kits
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.
No. of Reactions | 100 Reactions |
---|---|
Format | Kit |
Label or Dye | FAM + VIC |
Quantity | 100 reactions |
Host Cell | SF9 + Bacoluvirus |
PCR Method | qPCR |
Product Line | resDNASEQâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ TaqManâ„¢ Arbovirus Triplex Control Kit (ZIKV/DENV/CHIKV)
The Applied Biosystemsâ„¢ TaqMan Zika Virus Triplex Control Kit (ZIKV/DENV/CHIKV) includes a single foil pouch that contains human DNA at 1X and pooled complete genome RNA template of multiple viruses.
Applied Biosystemsâ„¢ resDNASEQâ„¢ Quantitative E. coli DNA Kits
This quantitative PCR (qPCR)-based kit, part of the resDNASEQâ„¢ E. coli Residual DNA Quantitation System, enables highly sensitive quantitation of host cell DNA from E. coli expression systems, typically in less than 4 hours.
No. of Reactions | 100 Reactions |
---|---|
Format | Kit |
Label or Dye | FAM |
Quantity | 100 reactions |
Host Cell | E.coli |
PCR Method | qPCR |
Product Line | resDNASEQâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ resDNASEQâ„¢ Quantitative Vero DNA Kits
The resDNASEQ Quantitative Vero DNA Kit is a quantitative PCR (qPCR)-based system for the detection of host cell DNA from Vero (African Green Monkey) cells, an expression system commonly used for the production of vaccines.
No. of Reactions | 100 Reactions |
---|---|
Format | Kit |
Label or Dye | FAM |
Quantity | 100 reactions |
Host Cell | Vero |
PCR Method | qPCR |
Product Line | resDNASEQâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ PureQuant Monocyte Assay
The Applied Biosystems PureQuant assays are a family of assays that accurately identify and quantify specific immune cell types in a mixed population of cells. These assays are TaqMan qPCR-based and measure genomic DNA methylation at specific sites that serve as a unique identifier. The assays include standards and controls and each 96-well plate can accommodate up to seven test samples.
Applied Biosystemsâ„¢ resDNASEQâ„¢ Quantitative Sf9 and Baculovirus DNA Kit with PrepSEQâ„¢ Residual DNA Sample Preparation Kit, 100 reactions
The resDNASEQ Quantitative Sf9 and Baculovirus DNA Kit is a duplex quantitative PCR (qPCR)-based system for the detection of residual DNA from the Sf9 baculovirus platform.
Applied Biosystemsâ„¢ TaqCheckâ„¢ SARS-CoV-2 Control Dilution Buffer
Applied Biosystems TaqCheck SARS-CoV-2 Control Dilution Buffer is required for use of the TaqCheck SARS-CoV-2 Fast PCR Assay. It is used to dilute the RNA control.
Applied Biosystemsâ„¢ ViralSEQâ„¢ Quantitative Sf-Rhabdovirus Kits
The Applied Biosystems ViralSEQ Quantitative Sf-Rhabdovirus Kit is a two-step RT-qPCR assay for the detection of Sf-rhabdovirus in recombinant products manufactured in insect cell lines using baculovirus expression systems.
Shipping Condition | Dry Ice |
---|---|
Target | Sf-rhabdovirus |
Quantity | 100 reactions |
PCR Method | qPCR |
Product Line | ViralSEQâ„¢ |
Detection Method | Primer-probe |
Applied Biosystemsâ„¢ QuantStudioâ„¢ Absolute Qâ„¢ Digital PCR Starter Kit
The QuantStudio Absolute Q Digital PCR Starter Kit is designed to confirm the performance of the QuantStudio Absolute Q Digital PCR System either at the time of system installation or as part of future, user-defined system verification procedures.