PCR Equipment and Supplies

Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents

Thermo Scientific™ Fluorescein-12-dUTP Solution (1 mM)

Thermo Scientific Fluorescein-12-dUTP (fluorescein-5(6)-carboxaminocaproyl-[5-{3-aminoallyl}-2'-deoxyuridine-5-triphosphate]) is supplied as 1 mM aqueous solution.

Thermo Scientific™ dCTP Solution (100 mM)

Thermo Scientific dCTP (2'-deoxycytidine 5'-triphosphate) is highly stable nucleotide, supplied as 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH.

Thermo Scientific™ dNTP/dUTP Mix

Thermo Scientific dNTP/dUTP Mix is a solution containing dATP, dCTP and dGTP, each at a final concentration of 2 mM and dUTP of 4 mM.

Invitrogen™ dCTP (100 mM)  

Invitrogen dCTP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dCTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.

Thermo Scientific™ dNTP Mix (10 mM each)

Thermo Scientific dNTP Mix (10 mM) contains premixed aqueous solutions of dATP, dCTP, dGTP and dTTP, each at a final concentration of 10 mM.

Invitrogen™ M13 Reverse Primer  

Oligonucleotides complementary to a DNA template are necessary to prime DNA synthesis for sequencing reactions.

Invitrogen™ Random Decamers (50 μM)  

Random Decamers are the same primers currently included in the RETROscript™ Kit (Cat. No. AM1710). They are provided at a stock concentration of 50 μM, and are functionally tested using the RETROscript™ Kit.

Invitrogen™ Lambda Hind III dsDNA Markers  

Generates fragments of the sizes 23,130, 9416, 6557, 4361, 2322, 2027, 564, and 125 bp.

Invitrogen™ 5-(3-Aminoallyl)-UTP (50 mM)  

Ambion modified nucleotides confer unique characteristics to the RNA molecules into which they are incorporated.

Thermo Scientific™ Oligo(dT)₁₈ Primer

Thermo Scientific Oligo(dT)₁₈ Primer is a synthetic single-stranded 18-mer oligonucleotide with 5'- and 3'-hydroxyl ends.

MilliporeSigma™ Novagen™ Oligo(dT) primer

Used for first-strand synthesis of cDNAs, using poly-adenylated mRNA as template

Applied Biosystems™ GeneAmp™ dNTP Blend (2.5 mM ea)  

GeneAmp dNTPs are deoxynucleotide triphosphates dissolved in glass-distilled water and titrated to pH 7.0 with NaOH. Each vial consists of 1 mL of 2.5 mM each of dATP, dCTP, dGTP, and dTTP, yielding a 10 mM blend of dNTPs.

Invitrogen™ dTTP (10 mM)  

Invitrogen dTTP is prepared to a final concentration of 10 mM in 0.9 mM Tris-HCl at pH 7.5.

Invitrogen™ dGTP (100 mM)  

Invitrogen dGTP is prepared to a final concentration of 100 mM in highly purified water. 100 mM dGTP is suitable for use in polymerase chain reaction (PCR), sequencing, fill-in reactions, nick translation, cDNA synthesis, and TdT-tailing reactions.