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Thermo Scientific™ Phusion High-Fidelity DNA Polymerase

Get superior performance for cloning and other applications requiring high fidelity with one of the most accurate thermostable DNA polymerases available.

$85.84 - $861.00

Products
Catalog Number Mfr. No. Includes Quantity Price Quantity    

F531L

 
thermo scientific™
F-531L
With High-Fidelity buffer 500 x 50uL Rxns Each for $861.00

F531S

 
thermo scientific™
F-531S
With High Fidelity buffer 100 x 50uL Rxns Each for $215.25

F532L

 
thermo scientific™
F-532L
With GC Buffer 500 x 50uL Rxns Each for $861.00

F532S

 
thermo scientific™
F-532S
With GC Buffer 100 x 50uL Rxns Each for $215.25

F530S

 
thermo scientific™
F-530S
Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. 100U (2U/µL) Each for $130.20

F530L

 
thermo scientific™
F-530L
Phusion High-Fidelity DNA Polymerase is supplied with 5x Phusion HF Buffer, 5x Phusion GC Buffer, DMSO and 50 mM MgCl2 solution. Both Phusion HF Buffer and Phusion GC Buffer provide 1.5 mM MgCl2 in the final 1x concentration. 500U (2U/µL) Each for $520.80

F553S

 
thermo scientific™
F-553S
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS 50 x 50µL Rxns (125 x 20µL Rxns) Each for $85.84

F553L

 
thermo scientific™
F-553L
Phusion DNA Polymerase, 5x Phusion HF Buffer, 5x Phusion GC Buffer, dNTP Mix, MgCl2 solution, control lambda DNA, 1.3kb control primers, 10kb control primers, DNA size standard, DMS 200 x 50µL Rxns (500 x 20µL Rxns) Each for $289.80
Description & Specifications

Phusion DNA Polymerases set a gold standard for PCR performance. They offer a combination of characteristics that no other enzyme can match. Featuring an error rate 50-fold lower than that of Taq, and 6-fold lower than that of Pfu, Phusion DNA Polymerase is the most accurate thermostable polymerase available. This feature makes Phusion DNA Polymerases superior choice for cloning and other applications requiring high fidelity. The unique structure of the enzyme enables short protocol times, abundant yields and robust performance, even in the presence of PCR inhibitors. For the researcher, that translates to ease-of-use and convenience. In addition, Phusion DNA Polymerases also produce higher yields with lower enzyme amounts than other DNA polymerases.

New Phusion Green format is a combination of Phusion DNA Polymerase and 5X Phusion Green Buffers. The buffers include a density reagent and two tracking dyes for direct loading of PCR products on a gel. The colored buffer does not interfere with excelent performance of Phusion DNA Polymerases and is compatible with downstream applications such as DNA sequencing, ligation and restriction digestion.

Phusion High-Fidelity PCR Master Mixes are convenient 2X mixes designed to minimize the number of pipetting steps. Only template and primers need to be added by the user. Phusion High-Fidelity PCR Kit contains all the necessary reagents for PCR including control template and primers.

Highlights

  • Extreme fidelity (error rate 4.4 x 10-7 in Phusion HF Buffer)
  • Polymerase speed allows short extension times (15 to 30 s/kb)
  • Robust performance, minimal optimization needed
  • Increased product yields with minimal enzyme amounts
  • Direct loading on gel with Green Buffer