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Invitrogen™ Platinum™ Taq DNA Polymerase DFS Item

Convenient and reliable “hot start” thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase

$114.45 - $4,347.00

Specifications

Overhang 3'-A
Size (Final Product) 10 kb or less
Storage Requirements Store at -30° to -10°C. Do not store in a frost-free freezer.
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Includes:  Each box contains: Platinum Taq DNA Polymerase; 10X PCR Buffer (no magnesium chloride); 50mM magnesium chloride; KB Extender

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

10966083

DFS Item
 
life technologies
10966083
5000 reactions Each for $4,347.00

10966018

DFS Item
 
life technologies
10966018
120 reactions Each for $114.45

10966034

DFS Item
 
life technologies
10966034
600 reactions Each for $527.10

10966026

DFS Item
 
life technologies
10966026
300 reactions Each for $298.20
Description & Specifications

Specifications

Overhang 3'-A
Size (Final Product) 10 kb or less
Storage Requirements Store at -30° to -10°C. Do not store in a frost-free freezer.

Use Platinum Taq DNA Polymerase for the amplification of DNA from complex genomic, viral, and plasmid templates, as well as in RT-PCR. The “hot start” property of the enzyme preparation is conferred by thermolabile monoclonal antibodies that render Taq DNA polymerase inactive until the initial PCR denaturation step.

  • Convenience: assemble PCR reactions at room temperature
  • Specificity: “hot start” kinetics reduce nonspecific primer annealing, improving product yield
  • Product size: use for PCR products up to 10kb
  • Flexibility: optional KB Extender gives more versatility in PCR assays

Using PlatinumTaq DNA Polymerase

  • Just as with Taq DNA Polymerase, Platinum Taq DNA Polymerase has a nontemplate-dependent terminal transferase activity that adds a 3' deoxyadenosine to product ends, and has a 5'→3' exonuclease activity (but not 3'→5' exonuclease activity)
  • PCR products generated with PlatinumTaq DNA Polymerase may be used in the same downstream applications without protocol modifications

Amplification of Bisulfite-Treated DNA, Chromatin Biology, Chromatin Immunoprecipitation (ChIP), Fast PCR, Hot Start PCR, Methylation Analysis, PCR, PCR and Real-Time PCR, RNAi, Epigenetics and Noncoding RNA Research, Routine PCR

Shipping Condition: Approved for shipment on wet or dry ice