Cell Based Assays
Cell Based Assays
Invitrogen™ JC-9 Dye (Mitochondrial Membrane Potential Probe)
Mitochondrial membrane potential probe
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
Invitrogen™ DAPI and Hoechst Nucleic Acid Stains
DAPI and Hoechst are classic popular nuclear counterstains for use in all fluorescent cell and tissue techniques. The blue fluorescence is vivid contrast to green, yellow, orange, red, far red and NIR fluorescent probes and labels.
Invitrogen™ NucBlue™ Fixed Cell ReadyProbes™ Reagent (DAPI)
High-purity form of DAPI stain in room temperature-stable solution that is provided in dropper bottle
Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Useful with a variety of fluorescence-detection methodologies
Invitrogen™ XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)
Redox-based viability probe
Invitrogen™ MitoSOX™ Mitochondrial Superoxide Indicators, for live-cell imaging
MitoSOX superoxide indicators are novel fluorogenic dyes specifically targeted to mitochondria in live cells. Oxidation of the MitoSOX reagent by mitochondrial superoxide produces bright green or red fluorescence.
Invitrogen™ ATP Determination Kit
Detect and quantify ATP in biochemical and cell viability assays with the ATP Determination Kit. This luminescence-based ATP assay utilizes the luciferase-luciferin reaction, offering exceptional sensitivity to detect as little as 0.1 picomole of ATP using a luminometer or microplate reader.
Invitrogen™ eBioscience™ 7-AAD Viability Staining Solution
Ready-to-use solution for the exclusion of nonviable cells in flow cytometric analysis. Can be used in combination with PE- and FITC-conjugated antibodies in 2-color analysis. The advantage of 7-AAD over PI is that there is minimal spectral overlap between these emissions.
Invitrogen™ CM-H2DCFDA (General Oxidative Stress Indicator)
General oxidative stress indicator
Invitrogen™ CyQUANT™ LDH Cytotoxicity Assay
CyQUANT™ LDH Cytotoxicity Assay Kit is a colorimetric assay that provides a simple and reliable method for determining cellular cytotoxicity.
Invitrogen™ Alexa Fluor™ 488 Phalloidin
High-affinity filamentous actin (F-actin) probe conjugated to bright, photostable, green-fluorescent Alexa Fluor 488 dye
Molecular Probes™ Fluo-4, AM, cell permeant
Fluo-4 is an analog of fluo-3 with the two chlorine substituents replaced by fluorines, which results in increased fluorescence excitation at 488 nm and consequently higher fluorescence signal levels.
Molecular Probes™ SYTOX™ Blue Dead Cell Stain, for flow cytometry
SYTOX™ Blue dead cell stain is a simple and quantitative single-step dead-cell indicator for use with violet laser equipped flow cytometers.
Molecular Probes™ PrestoBlue™ Cell Viability Reagent
PrestoBlue™ Cell Viability Reagent