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Molecular Probes™ Annexin V Conjugates for Apoptosis Detection DFS Item

Detect early stages of apoptosis with Annexin V stand-alone Alexa Fluor, APC, Pacific Blue, PE, FITC, and biotin conjugates using flow cytometry.

$558.00 - $860.00

Specifications

Product Type Annexin V conjugate
Shipping Condition Wet Ice
For Use With (Equipment) Flow Cytometer
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Products 10
Catalog Number Mfr. No. Excitation/Emission Flow Cytometer Laser Lines Conjugate Price Quantity  
Catalog Number Mfr. No. Excitation/Emission Flow Cytometer Laser Lines Conjugate Price Quantity  
A13201 DFS Item
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Molecular Probes™
A13201
495/519 488 Alexa Fluor 488
Each for $636.00
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A13199 DFS Item
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Molecular Probes™
A13199
494/518 488 FITC
Each for $558.00
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501121497 DFS Item
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Molecular Probes™
A13202
578/603 532, 561 Alexa Fluor 568
Each for $636.00
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A13203 DFS Item
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Molecular Probes™
A13203
590/617 532 Alexa Fluor 594
Each for $640.00
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501121498 DFS Item
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Molecular Probes™
A13204
Biotin-X
Each for $718.00
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A23204 DFS Item
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Molecular Probes™
A23204
650/665 633-637 Alexa Fluor 647
Each for $646.00
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A35108
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Molecular Probes™
A35108
555/565 532, 561 Alexa Fluor 555
Each for $694.00
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501121504 DFS Item
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Molecular Probes™
A35109
679/702 633-637 Alexa Fluor 680
Each for $670.00
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501137399 DFS Item
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Molecular Probes™
A35111
565/578 488, 532, 561 PE
Each for $860.00
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501121505 DFS Item
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Molecular Probes™
A35122
410/455 405 Pacific Blue
Each for $666.00
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Description

Description

Achieve quick and reliable detection of early cell apoptosis with Annexin V stand-alone conjugates for apoptosis detection. Annexin V conjugates offer up to 100-fold difference in fluorescence signal intensity between apoptotic and non-apoptotic cells using flow cytometry.

Annexin V has a high affinity for phosphatidylserine (PS), which becomes exposed on the outer leaflet of cells undergoing apoptosis. Because of this affinity, fluorescently labeled annexin V reagents are commonly used in apoptosis research.

Annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, an indicator of intermediate stages of apoptosis. The difference in fluorescence intensity between apoptotic and nonapoptotic cells stained with our fluorescent annexin V conjugates, as measured by flow cytometry, is typically about 100-fold.

In collaboration with Nexins Research BV, we provide the best and brightest annexin V conjugates available, including Alexa Fluor 350, 488, 555, 568, 594, 647, and 680 annexin V conjugates, as well as Annexin V APC, Biotin-X, FITC, Pacific Blue, and PE conjugates. Highly fluorescent annexin V conjugates provide quick and reliable detection methods for studying the externalization of phosphatidylserine, one of the earliest indicators of apoptosis.

The Annexin V Pacific Blue conjugate is violet excitable, making it ideal for instruments with a violet laser and for multicolor experiments that include green- or red-fluorescent dyes.

The benefits of our annexin V conjugates include:
• Conjugated to Invitrogen Alexa Fluor and eFluor dyes for brighter signals
• Conjugates for all available lasers
• Available as stand-alone reagents or easy-to-use kits

Annexin V staining to detect apoptotic cells can only be done on live cells and tissue. If samples are to be fixed post-staining, there are specific conditions required to achieve transient retention of signal. These include use of an alcohol-free, aldehyde-based fixation method, use of buffers containing Ca2+ and avoidance of surfactants/detergents. For your convenience, we also offer a concentrated annexin-binding buffer that facilitates the binding of annexin V to phosphatidylserine in apoptosis assays.

Specifications

Specifications

Annexin V conjugate
Wet Ice
Flow Cytometer
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