PCR Equipment and Supplies
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
| Physical Form | Liquid |
|---|---|
| pH | 7 |
| Chemical Name or Material | Saline-Sodium Citrate |
| Grade | Molecular Biology |
| DNase | DNase free |
| Recommended Storage | RT |
| Concentration | 2.9 to 3.1 M |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| GC-Rich PCR Performance | Medium |
| Content And Storage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Hot Start PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR |
Invitrogen™ Platinum™ Taq DNA Polymerase, High Fidelity
Platinum Taq DNA Polymerase, High Fidelity, is ideal for amplification of DNA fragments when high yields and robust amplification are required.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 6X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Invitrogen™ SuperScript IV UniPrime One-Step RT-PCR System
Invitrogen SuperScript IV UniPrime One-Step RT-PCR System is a convenient one-step formulation for performing fast cDNA synthesis and PCR amplification in a single tube.
| GC-Rich PCR Performance | High |
|---|---|
| Format | 1-step RT-PCR Kit |
| Reverse Transcriptase | SuperScript IV |
| Size (Final Product) | Up to 13 kb |
| Reaction Format | Master Mix |
| Reagent Type | Reverse Transcription |
| Hot Start | Built-in Hot Start |
| Optimal Reaction Temperature | 50°C to 65°C |
| Technique | 1-step RT-PCR |
| Starting Material | RNA |
| PCR Method | 1-step RT-PCR |
| Multiplex Capability | Yes |
| Detection Method | Gel Electrophoresis |
Invitrogen™ SuperScript™ One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
SuperScript One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the convenient end-point detection and analysis of RNA molecules by RT-PCR.
Invitrogen™ Platinum™ Taq DNA Polymerase
Invitrogen Platinum Taq DNA Polymerase is a convenient and reliable 'hot start' thermostable DNA polymerase for PCR that provides enhanced specificity over that of Taq DNA Polymerase.
| Polymerase | Platinum Taq DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Format | Tube |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Starting Material | DNA |
| For Use With (Application) | Hot-start PCR |
| Exonuclease Activity | 5' - 3' |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Buffer II and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 5 kb or less |
| Product Type | DNA Polymerase |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| Exonuclease Activity | 5' - 3' |
Applied Biosystems™ AmpliTaq Gold™ DNA Polymerase with Gold Buffer and MgCl2
AmpliTaq Gold DNA Polymerase is a chemically modified form of AmpliTaq DNA Polymerase requiring thermal activation.
| Polymerase | AmpliTaq Gold DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Hot Start | Built-In Hot Start |
| Overhang | 3'-A |
| Concentration | 5 U/μL |
| Exonuclease Activity | 5' - 3' |
Invitrogen™ Platinum™ II Taq Hot-Start DNA Polymerase
Invitrogen Platinum II Taq Hot-Start DNA Polymerase is designed for universal primer annealing and fast, easy PCR with its unique combination of innovative buffer, high-performance engineered Taq DNA polymerase, and superior hot-start technology.
| Polymerase | Platinum II Taq Hot-Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 1X |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Product Type | Hot Start DNA Polymerase |
| Reaction Speed | Fast or Standard |
| Overhang | 3'-A |
| Concentration | 2X |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2 U/μL |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus PCR Master Mixes
Thermo Scientific Phusion Plus PCR Master Mix is a convenient, ready-to-use 2X mixture of Phusion Plus DNA Polymerase, buffer, magnesium chloride, and dNTPs.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion Plus DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >100X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Ready to use master mix |
| Reaction Speed | 15 to 30 sec./kb |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Master Mix with HF or GC Buffer
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion High-Fidelity DNA Polymerase |
| Fidelity (vs. Taq) | 52X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | High-Fidelity PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | No |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Standard PCR,High-fidelity PCR |
Thermo Scientific™ Phusion U Multiplex PCR Master Mixes
Thermo Scientific Phusion U Multiplex PCR Master Mix is a ready-to-use, 2X end-point PCR master mix designed for simultaneous amplification of multiple targets in a single tube.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Phusion U Hot Start DNA Polymerase |
| Fidelity (vs. Taq) | 25X |
| Size (Final Product) | 2.5 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | Multiplex PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerase & dNTP Mix (10 mM each)
This package includes Phusion Hot Start II High-Fidelity DNA Polymerase (4 x 500 U) and high quality dNTP Mix (10 mM each, 2 x 1 mL).