PCR Equipment and Supplies
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
| Physical Form | Liquid |
|---|---|
| pH | 7 |
| Chemical Name or Material | Saline-Sodium Citrate |
| Grade | Molecular Biology |
| DNase | DNase free |
| Recommended Storage | RT |
| Concentration | 2.9 to 3.1 M |
Invitrogen™ ROX Reference Dye
ROX Reference Dye is designed to normalize the fluorescent reporter signal in real-time quantitative PCR or RT-PCR.
| Content And Storage | Store in freezer (-5° to -30°C). |
|---|---|
| Label or Dye | ROX |
| Detection Method | Primer-probe |
Invitrogen™ AccuPrime™ GC-Rich DNA Polymerase
AccuPrime GC-Rich DNA Polymerase is designed to provide high-yield, high-specificity amplification of difficult-to-amplify templates, such as those with >65% GC content.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | AccuPrime GC-Rich DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 2X |
| Size (Final Product) | 5 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR |
Invitrogen™ AccuPrime™ Pfx SuperMix
AccuPrime Pfx SuperMix is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Invitrogen™ AccuPrime™ Taq DNA Polymerase, High Fidelity
Invitrogen AccuPrime Taq DNA Polymerase, High Fidelity, provides qualified reagents for the high-fidelity PCR amplification of nucleic acid templates.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | AccuPrime Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 9X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Separate Components |
| Product Type | DNA Polymerase |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |
Invitrogen™ Platinum™ SuperFi™ PCR Master Mix
Invitrogen Platinum SuperFi PCR Master Mix is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | 300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi II DNA Polymerase
Invitrogen Platinum SuperFi II DNA Polymerase is a proofreading DNA polymerase that combines superior fidelity with an innovative buffer, enabling universal primer annealing for the highest success in PCR.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | Standalone |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ Platinum™ SuperFi II PCR Master Mixes
Invitrogen Platinum SuperFi II PCR Master Mix (2X) is a ready-to-use mixture of Platinum SuperFi II DNA Polymerase, Platinum SuperFi II Buffer, and dNTPs for convenient PCR setup and efficient amplification.
| Shipping Condition | Dry Ice |
|---|---|
| Polymerase | Platinum SuperFi II DNA Polymerase |
| GC-Rich PCR Performance | High |
| Fidelity (vs. Taq) | >300X |
| Size (Final Product) | 20 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Product Type | PCR Master Mix |
| Reaction Speed | Fast |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| Concentration | 2X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ AccuPrime™ SuperMix II
AccuPrime SuperMix II is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Invitrogen™ AccuPrime™ SuperMix I
AccuPrime SuperMix I is a ready-to-use mixture of DNA polymerase, accessory proteins, salts, magnesium, and dNTPs for efficient PCR amplification.
Invitrogen™ AccuPrime™ Pfx DNA Polymerase
AccuPrime Pfx DNA Polymerase is ideal for high-fidelity amplification of DNA fragments for downstream applications such as cloning and mutagenesis.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | AccuPrime Pfx DNA Polymerase |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 26X |
| Size (Final Product) | 12 kb or less |
| Reaction Format | Separate Components |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | Blunt |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
| Detection Method | Primer-probe |
Invitrogen™ Platinum™ PCR SuperMix High Fidelity
Platinum PCR SuperMix, High Fidelity, is a ready-to-use mixture of DNA polymerase, salts, magnesium, and dNTPs for efficient PCR amplification.
| Shipping Condition | Wet or Dry Ice |
|---|---|
| Polymerase | Platinum Taq DNA Polymerase High Fidelity |
| GC-Rich PCR Performance | Low |
| Fidelity (vs. Taq) | 6X |
| Size (Final Product) | 15 kb or less |
| Reaction Format | SuperMix or Master Mix |
| Reaction Speed | Standard |
| Hot Start | Built-In Hot Start |
| Overhang | Mixed |
| Concentration | 1.1X |
| For Use With (Application) | Hot-start PCR,High-fidelity PCR |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq DNA Polymerase is designed for the sensitive, reproducible, end-point detection and analysis of RNA molecules by RT-PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq DNA Polymerase |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 4.5 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Invitrogen™ SuperScript™ III One-Step RT-PCR System with Platinum™ Taq High Fidelity DNA Polymerase
The SuperScript III One-Step RT-PCR System with Platinum Taq High Fidelity is designed for convenient end-point detection and analysis of RNA molecules by one-step RT-PCR.
| Shipping Condition | Dry Ice |
|---|---|
| GC-Rich PCR Performance | High |
| Reverse Transcriptase | SuperScript III |
| Final Product Type | PCR Amplified cDNA |
| PCR Method | 1-step RT-PCR |
| Detection Method | Gel Electrophoresis |
| Polymerase | Platinum Taq High Fidelity |
| Format | One-Step RT-PCR System |
| Size (Final Product) | Up to 10 kb |
| Reaction Format | Premixed Components |
| Reaction Speed | 30 min. |
| Reagent Type | Reverse Transcription |
| Ribonuclease H Activity | Reduced |
| Hot Start | Built-In Hot Start |
| Optimal Reaction Temperature | 50°C |
| Technique | 1-Step RT-PCR |
| Starting Material | RNA |
Invitrogen™ Anchored Oligo(dT)20 Primer
Anchored Oligo(dT)20 Primer is a primer mixture consisting of a string of 20 deoxythymidylic acid residues followed by dV (dG, dA, or dC) and then by dN (dA, dT, dG, or dC).