PCR Equipment and Supplies
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Saline-Sodium Citrate (SSC), 20X Solution (Molecular Biology), Fisher BioReagents
| Nom chimique ou matériau | Saline-Sodium Citrate |
|---|---|
| Qualité | Molecular Biology |
| pH | 7 |
| DNase | DNase free |
| Concentration | 2.9 to 3.1 M |
| Forme physique | Liquid |
| Température de stockage | RT |
Thermo Scientific™ Phusion U Hot Start PCR Master Mix
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Polymérase | Phusion U Hot Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | Medium |
| Fidélité (par rapport à Taq) | 25X |
| Vitesse de réaction | Fast |
| Format de réaction | SuperMix or Master Mix |
| Type de produit | Hot Start PCR Master Mix |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Concentration | 2X |
| Taille (produit final) | 20 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR |
| Contenu et stockage | • Phusion U Hot Start DNA Polymerase • 2X reaction buffer • 3 mM MgCl2 • 400 μM each of dATP, dTTP, dCTP and dGTP Master mix is supplied with nuclease-free water. Store at -20°C. |
Thermo Scientific™ Phusion™ Hot Start II DNA Polymerases (2 U/μL)
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| GC-Rich PCR Performance | High |
|---|---|
| Fidélité (par rapport à Taq) | 52X |
| Vitesse de réaction | Fast |
| Format de réaction | Standalone |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Concentration | 2 U/μL |
| Taille (produit final) | 20 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion High-Fidelity PCR Kit
Thermo Scientific Phusion High-Fidelity DNA polymerases set a gold standard for high performance PCR.
| Polymérase | Phusion High-Fidelity DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 52X |
| Vitesse de réaction | Fast |
| Format de réaction | Separate Components |
| Type de produit | High-Fidelity PCR Kit |
| Hot Start | No |
| En surplomb | Blunt |
| Taille (produit final) | 20 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Standard PCR,High-fidelity PCR |
| Contenu et stockage | • Phusion DNA Polymerase (2 U/μL) • 5X Phusion GC & HF Buffer • dNTP mix (10 mM each) • Control template (λ DNA) and DNA size standard • 1.3 kb & 10 kb primers (4 μM each) • 50 mM MgCl2 solution and DMSO Store at –20°C. |
Thermo Scientific™ Phusion U Hot Start DNA Polymerases
Thermo Scientific Phusion U DNA polymerase is a novel engineered high fidelity enzyme developed using fusion technology.
| Polymérase | Phusion U Hot Start DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | 25X |
| Vitesse de réaction | Fast |
| Format de réaction | Standalone |
| Type de produit | Hot Start DNA Polymerase |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Concentration | 2 U/μL |
| Taille (produit final) | 20 kb or less |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Phusion™ Plus DNA Polymerase
Thermo Scientific Phusion Plus DNA Polymerase is a hot-start, high-fidelity DNA polymerase that brings together protein fusion technology and universal primer annealing.
| Polymérase | Phusion Plus DNA Polymerase |
|---|---|
| GC-Rich PCR Performance | High |
| Fidélité (par rapport à Taq) | >100X |
| Vitesse de réaction | 15 to 30 sec./kb |
| Format de réaction | Separate components |
| Hot Start | Built-In Hot Start |
| En surplomb | Blunt |
| Taille (produit final) | 20 kb or less |
| Conditions d’expédition | Room Temp or Wet Ice |
| À utiliser avec (application) | Hot-start PCR,High-fidelity PCR |
Thermo Scientific™ Mutation Generation System Kit
Perform functional analysis of proteins with these transposon-based tools.
Thermo Scientific™ MuA Transposase (concentrated)
Perform functional analysis of proteins with these transposon-based tools.
Applied Biosystems™ QuantStudio™ Absolute Q™ AutoRun dPCR Suite, single configuration system
The Applied Biosystems QuantStudio Absolute Q AutoRun Digital PCR Suite is an integrated system solution that scales precise nucleic acid quantification and offers flexible instrument configurations and intelligent automation support for up to 72 hours of hands-free operation.
Applied Biosystems™ VeritiPro™ Dx Thermal Cycler, 96 well, IQ-OQ, Extended Care
The Applied Biosystems VeritiPro Dx Thermal Cycler is a next-generation end-point thermal cycler designed to amplify nucleic acids using the polymerase chain reaction (PCR). The VeritiPro Dx Thermal Cycler is both US-IVD and CE-IVD, IVDR compliant.
Applied Biosystems™ MycoSEQ™ Plus Mycoplasma Detection Kits
The MycoSEQ™ Plus Mycoplasma Detection Kit is a TaqMan™-based quantitative PCR (qPCR) kit for the detection of mycoplasma in complex bioproduction samples.
| GC-Rich PCR Performance | Medium |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Standard |
| Type de produit | Mycoplasma Detection |
| Nbre de réactions | 100 Reactions |
| Gamme de produits | MycoSEQ |
| Type d’échantillon | Biopharmaceutical Purification Sample, Bulk Drug Substance Sample, Cell Cultures |
| Classe d’organisme cible | Mollicutes, mycoplasma |
| Méthode PCR | Multiplex qPCR |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Microbial Detection |
| Marqueur ou colorant | FAM |
| Forme | Liquid |
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| Concept écologique | REACH compliant, less waste |
|---|---|
| Méthode de détection | TaqMan |
| Technologie d’isolement | Direct cell lysis |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | RNA |
| Méthode PCR | 2-step RT-qPCR |
| Conditions d’expédition | Frozen, Dry Ice |
| Type de kit | Direct Cell Lysis RT-qPCR |
| À utiliser avec (application) | Gene Expression |
| Contenu et stockage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
| Compatibilité à haut débit | Yes |
| Format | Kit |
| Cible | cDNA |
| Matériau de départ | 10-100,000 Cells |
Applied Biosystems™ SteriSEQ™ Rapid Sterility Testing Kit
The SteriSEQ Rapid Sterility Testing Kit is a TaqMan-based quantitative PCR (qPCR) kit for the detection of bacteria and fungi in complex bioproduction samples.
| Méthode de détection | qPCR |
|---|---|
| Gamme de produits | SteriSEQ |
| Méthode PCR | qPCR |
| À utiliser avec (application) | Real Time PCR (qPCR) |
| Marqueur ou colorant | FAM + VIC,ABY,Alexa Fluor 647 |
| Forme | Liquid |
| Contenu et stockage | • SteriSEQ™ Assay Mix, 2 x 110 μL; Store at -25°C to -15°C and protect from light • qPCR Master Mix Plus, 2 x 750 μL; Store at -25°C to -15°C until first thawed, then store at 2°C to 8°C and protect from light • SteriSEQ™ Discriminatory Positive Control, 2 x 30 μL; Store at -25°C to -15°C • SteriSEQ™ DNA Dilution Buffer, 1 x 7 mL; Store at -25°C to -15°C until first thawed, then store at room temperature |
Thermo Scientific™ SureTect™ Beverage Spoilage Multiplex PCR Assay
Rapid qPCR solutions for beverage manufacturers. Rapidly detect a broad range of beverage spoilers from one PCR reaction with this assay.
Applied Biosystems™ VetMAX™ Fast Multiplex Mastermix, with ROX
VetMAX Fast Multiplex Master Mix with ROX is designed to be a fast-cycling-capable, multiplex-assay-compatible one-step RT-PCR master mix for animal molecular diagnostics.
| Polymérase | AmpliTaq™ Fast DNA Polymerase |
|---|---|
| Méthode de détection | Primer-probe |
| Vitesse de réaction | Fast |
| Durée d’activation | 5 min. |
| Hot Start | Built-in Hot Start |
| Type de produit final | Mastermix |
| Méthode PCR | 1-step RT-qPCR |
| Concentration | 2X |
| Compatibilité | TaqMan™ |
| Matériau de départ | DNA/RNA |
| À utiliser avec (application) | Animal Health |
| Marqueur ou colorant | ROX |
| Forme | Liquid |