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Apoptosis Reagents and Kits

Assays, reagents, kits, and other assorted products used to detect cellular apoptosis, determine the activity levels of caspase or cathepsin, or identify similar cellular activity.
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1

Invitrogen™ CellEvent™ Caspase-3/7 Detection Reagents  DFS Item

CellEvent Caspase-3/7 detection reagents are novel fluorogenic substrates for apoptosis detection. These substrates detect activated caspase-3/7 in live cells that can be fixed and multiplexed with antibody detection workflows.

Detection Method Fluorescence
2

Invitrogen™ Apo-BrdU Apoptosis Detection Kit

Contains the instructions and reagents required for measuring apoptosis in cells. APO-BRDU™ Apoptosis Detection Kit is a 2-color staining method for labeling DNA breaks and total cellular DNA to detect apoptotic cells by flow cytometric analysis in immunochemistry applications.

3

Invitrogen™ CellEvent™ Caspase-3/7 Green ReadyProbes™ Reagent  DFS Item

Fluorogenic, no-wash indicator for live- and fixed-cell applications

4

Invitrogen™ CaspGLOW™ Fluorescein Active Caspase-9 Staining Kit

Contains all the reagents necessary to detect active Caspase-9 in mammalian cells with high sensitivity. Detection of the labeled cells can be determined by flow cytometry, fluorescent microscopy or a fluorescent plate reader.

5

Invitrogen™ Annexin V Recombinant Protein, FITC, eBioscience™

Recombinant Protein

Purity or Quality Grade >89% by SDS-PAGE and RP-HPLC
Conjugate FITC
Specific Reactivity All
Form Liquid
pH Range 7.4
Common Name Annexin V
Molecular Weight (g/mol) 35.8kDa
For Use With (Application) Neutralization,Flow Cytometry
Source E. coli
Name Annexin V
Purification Method Purified
Biological Activity Annexin V exhibits anti-phospholipase activity and binds to phosphatidylserine (PS).
Formulation TBS with 1% BSA and 0.02% sodium azide; pH 7.4
Species E. coli
Recombinant Recombinant
Gene Symbol Anxa5
Storage Requirements 4°C, store in dark, DO NOT FREEZE!
Concentration 5 μL/Test
Protein Subtype Recombinant
Accession Number P08758, P14668, P48036
Regulatory Status RUO
Product Type Protein
Gene ID (Entrez) 11747, 25673, 308
Structural Form E. Coli
Shelf Life 12 Months
Cross Reactivity All
6

Molecular Probes™ Annexin V Conjugates for Apoptosis Detection  DFS Item

Detect early stages of apoptosis with Annexin V stand-alone Alexa Fluor, APC, Pacific Blue, PE, FITC, and biotin conjugates using flow cytometry.

Shipping Condition Wet Ice
For Use With (Equipment) Flow Cytometer
Product Type Annexin V conjugate
7

Molecular Probes™ CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit  DFS Item

The CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit enables the flow cytometric detection of activated caspase-3 and caspase-7 in apoptotic cells.

Shipping Condition Wet Ice
Content And Storage Contains 1 vial CellEvent™ Caspase 3/7 Green Detection Reagent, 1 vial SYTOX AADvanced dead cell stain, and 1 vial of DMSO.

Store all vials in the kit upright, at or below -20deg;C, desiccated, and protected from light.
Format Kit
For Use With (Equipment) Attune™ Acoustic Focusing Cytometer, Flow Cytometer
Flow Cytometer Laser Lines 488
Product Type Caspase Assay Kit
Label or Dye CellEvent™ Caspase 3/7 Green Detection Reagent, SYTOX AADvanced dead cell stain
Label Type Other Label(s) or Dye(s)
For Use With (Application) Flow Cytometry
Detection Method Fluorescence
Product Line CellEvent
8

Molecular Probes™ Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry  DFS Item

Mitochondrial Membrane Potential Apoptosis Kit, with Mitotracker™ Red & Annexin V Alexa Fluor™ 488, for flow cytometry

9

Molecular Probes™ Membrane Permeability Dead Cell Apoptosis Kit with PO-PRO™-1 and 7-Aminoactinomycin D, for flow cytometry  DFS Item

Vybrant™ Apoptosis Assay Kit #13 *PO-PRO™-1/7-aminoactinomycin D* *for flow cytometry* *200 assays*

11

Invitrogen™ Image-iT™ LIVE Red and Green Caspase Apoptosis Detection Kits for microscopy  DFS Item

Detect apoptosis via microscopy, including fluorescence microscopy and HCS, with Image-iT LIVE Red and Green Caspase Apoptosis Detection Kits that report poly and caspase-3/7 activities.

Shipping Condition Room Temperature
No. of Reactions 25 tests (labeling volumes of 300μL)
Format Slide
For Use With (Equipment) Fluorescence Microscope
Quantity 1 kit
Storage Requirements Store in refrigerator (2–8°C) and protect from light.
Label Type Other Label(s) or Dye(s)
Detection Method Fluorescence
Product Line Image-iT
12

Molecular Probes™ Metabolic Activity Dead Cell Apoptosis Kit with C12 Resazurin, Annexin V APC, and SYTOX™ Green, for flow cytometry  DFS Item

Vybrant™ Apoptosis Assay Kit #10 - allophycocyanin annexin V/C12-resazurin/SYTOX™ Green - 50 assays

13

Invitrogen™ Click-iT™ Plus TUNEL Assay Kits for In Situ Apoptosis Detection  DFS Item
GREENER_CHOICE

Detect apoptosis in cells and tissues samples with Click-iT Plus TUNEL Assay kits, which offer easy dye incorporation and can be multiplexed with GFP and RFP.

Shipping Condition Dry Ice
No. of Reactions 50 coverslips
Format Coverslip
For Use With (Equipment) Fluorescence Microscope
Product Type TUNEL Assay
Quantity 1 kit
Label Type Alexa Fluor™ Dyes
Detection Method Fluorescence
Product Line Click-iT
14

Molecular Probes™ Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry  DFS Item

Pacific Blue™ Annexin V/SYTOX™ AADvanced™ Apoptosis Kit, for flow cytometry