Cell Function Reagents and Kits
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Invitrogen™ XTT (2,3-Bis-(2-Methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide)
Redox-based viability probe
Invitrogen™ PrestoBlue™ and PrestoBlue™ HS Cell Viability Reagents
Monitor cell proliferation and viability with the resazurin-based PrestoBlue and PrestoBlue HS cell viability reagents. These nontoxic and cell permeable reagents quantitatively measure cell viability in as little as 10 minutes using absorbance- or fluorescence-based microplate readers.
| Content And Storage | Store at 2°C to 8°C and protect from light. |
|---|---|
| Form | Liquid |
| For Use With (Equipment) | Microplate Reader,Spectrophotometer,HTS Reader |
| For Use With (Application) | Viability Assay |
| Product Line | PrestoBlue |
| Detection Method | Colorimetric, Fluorescence |
Molecular Probes™ MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
MTT (3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide)
Invitrogen™ LIVE/DEAD™ BacLight™ Bacterial Viability Kits
LIVE/DEAD™ BacLight™ Bacterial Viability Kits are easy-to-use fluorescent staining kits commonly used for assessing the viability of bacterial cells. These kits provide a two-color fluorescence assay of bacterial viability using green and red fluorescent dyes for use with imaging, flow, and assays.
| Shipping Condition | Room Temperature |
|---|---|
| Product Type | Bacterial Viability Kit |
| Dye Type | Other Label(s) or Dye(s) |
| Product Line | BacLight, LIVE/DEAD |
| Detection Method | Fluorescence |
| Cell Type | Bacteria |
Invitrogen™ LIVE/DEAD™ Viability/Cytotoxicity Kit, for mammalian cells
Useful with a variety of fluorescence-detection methodologies
Invitrogen™ Calcein AM, cell-permeant green and blue dyes
Calcein AM is a cell-permeant dye that can be used to determine cell viability in most eukaryotic cells. In live cells, the non-fluorescent Calcein AM is converted to green or blue fluorescent Calcein, after acetoxymethyl ester hydrolysis by intracellular esterases.
| Shipping Condition | Room Temperature |
|---|---|
| Content And Storage | Store in freezer (-5°C to -30°C) and protect from light. |
| For Use With (Equipment) | Fluorescence Microscope |
| Reagent Type | Cell Tracker Compounds, Cell Labeling Reagents |
| Dye Type | Other Label(s) or Dye(s) |
| For Use With (Application) | Cell Tracing,Cell Tracker |
Molecular Probes™ LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation
The LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
| Shipping Condition | Room Temperature |
|---|---|
| dimExcitationWavelengthRange | 375 nm |
| Description | LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit, for 405 nm excitation |
| Form | Solid |
| Color | Aqua |
| Emission | 526 |
| Dye Type | LIVE/DEAD™ Fixable Aqua Dead Cell Stain |
| For Use With (Application) | Viability Assay |
| Detection Method | Fluorescence |
| Cell Type | Eukaryotic Cells |
| Cell Permeability | Impermeant |
| Product Type | Stain |
| For Use With (Equipment) | Flow Cytometer |
| Product Line | LIVE/DEAD |
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 660
Can be used to irreversibly label dead cells prior to cryopreservation, fixation and/or permeabilization procedures. Unlike 7-AAD and PI, cells labeled with Fixable Viability Dyes can be washed, fixed, permabilized and stained for intracellular antigens without loss of staining intensity.
| Product Type | Dye |
|---|---|
| For Use With (Equipment) | Flow Cytometer |
| Color | Red |
| Emission | 633/660 |
| For Use With (Application) | Viability Assay |
| Product Line | eBioscience |
| Detection Method | Fluorescence |
Invitrogen™ eBioscience™ Fixable Viability Dye eFluor™ 780
A viability dye that can be used to irreversibly label dead cells prior to cryopreservation, fixation, and/or permeabilization procedures.
| Product Type | Dye |
|---|---|
| For Use With (Equipment) | Flow Cytometer |
| Color | Infrared |
| Emission | 633/780 |
| For Use With (Application) | Viability Assay |
| Product Line | eBioscience |
| Detection Method | Fluorescence |
Invitrogen™ 5-CFDA, AM (5-Carboxyfluorescein Diacetate, Acetoxymethyl Ester)
A cell-permeant esterase substrate that can serve as a viability probe
Invitrogen™ EnzChek™ Gelatinase/Collagenase Assay Kit, 250-2,000 assays
Fluorometric method for measuring gelatinase or collagenase activity
Invitrogen™ CyQUANT™ MTT and XTT Cell Viability Assays
CyQUANT MTT and XTT Cell Viability assays are colorimetric microplate assays for accurate assessment of cell health, proliferation, and cytotoxicity. CyQUANT MTT is a well-established assay, while CyQUANT XTT offers improved performance and continuous measurement without cell lysis or solubilization.
| Format | 96-well plate |
|---|---|
| Product Type | Cell Viability Assay |
| For Use With (Equipment) | Microplate Reader |
| For Use With (Application) | Viability Assay,Proliferation Assay,Cytotoxicity Assay |
| Product Line | CyQUANT |
| Detection Method | Colorimetric |
| Cell Type | Mammalian Cells |
Invitrogen™ LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation
The LIVE/DEAD™ Fixable Far-Red Dead Cell Stain Kit is used to determine the viability of cells prior to the fixation and permeabilization required for intracellular antibody staining, or prior to elimination of biohazardous materials using formaldehyde fixation.
| Shipping Condition | Room Temperature |
|---|---|
| dimExcitationWavelengthRange | 633 nm |
| Description | LIVE/DEAD™ Fixable Far Red Dead Cell Stain Kit, for 633 or 635 nm excitation |
| Form | Solid |
| Solubility | DMSO (Dimethylsulfoxide) |
| Color | Far-red |
| Emission | 655 |
| Dye Type | LIVE/DEAD™ Fixable Far Red Dead Cell Stain |
| For Use With (Application) | Viability Assay |
| Detection Method | Fluorescence |
| Cell Type | Eukaryotic Cells |
| Cell Permeability | Impermeant |
| Format | Tube(s) |
| Product Type | Stain |
| For Use With (Equipment) | Flow Cytometer |
| Product Line | LIVE/DEAD |