Direct PCR Reagents and Kits

Direct PCR Reagents and Kits
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Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
Polymérase | Phire Hot Start II DNA Polymerase |
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GC-Rich PCR Performance | Medium |
Vitesse de réaction | Fast |
Format de réaction | SuperMix or Master Mix |
Type de produit | Direct PCR Master Mix |
Hot Start | Built-In Hot Start |
Type d’échantillon | Plant |
En surplomb | Blunt |
Méthode PCR | Direct PCR |
Concentration | 2X |
Matériau de départ | DNA |
Conditions d’expédition | Wet or Dry Ice |
À utiliser avec (application) | Genotyping,Hot-start PCR |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Durée de fonctionnement | 10 min. |
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Format | Kit |
Technologie d’isolement | Silica Spin Column |
Type d’échantillon | PCR Products |
Conditions d’expédition | Room Temperature |
Contenu et stockage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
GC-Rich PCR Performance | Medium |
---|---|
Vitesse de réaction | Fast |
Format de réaction | SuperMix or Master Mix |
Type de produit | Direct PCR Master Mix |
Type d’échantillon | Tissue |
En surplomb | Blunt |
Méthode PCR | Direct PCR |
Concentration | 2X |
Conditions d’expédition | Wet or Dry Ice |
À utiliser avec (application) | Genotyping,Hot-start PCR |
Comprend l’étiquette ou le colorant | Yes |
Polymérase | Phire Hot Start II DNA Polymerase |
Hot Start | Built-In Hot Start |
Matériau de départ | DNA |
Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer
These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.
Invitrogen™ Single Cell Lysis Kit
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
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Durée de fonctionnement | 5 min. |
Format | Spin Column |
Contenu et stockage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Méthode de détection | Primer-probe |
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À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Vitesse de réaction | Fast or Standard |
Gamme de produits | CellsDirect™, Platinum™, SuperScript™ |
Type d’échantillon | Cells |
Concentration | 2X |
À utiliser avec (application) | RT-PCR |
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Méthode de détection | Primer-probe |
---|---|
À utiliser avec (équipement) | 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Vitesse de réaction | Fast or Standard |
Gamme de produits | CellsDirect™, Platinum™, SuperScript™ |
Type d’échantillon | Cells |
Concentration | 2X |
À utiliser avec (application) | RT-PCR |
Invitrogen™ GeneArt™ Genomic Cleavage Detection Kit
The GeneArt Genomic Cleavage Detection Kit provides a simple, reliable, and rapid method for the detection of locus-specific double-strand break formation.
Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
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Concept écologique | Fewer resources used and less waste, Less hazardous |
Méthode de détection | TaqMan |
Type de réactif | Cell Lysis |
À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Vitesse de réaction | 7 min. |
Format | Tube |
Type de produit | 1-Step qPCR kit |
Gamme de produits | Cells-to-CT™, TaqMan™ |
Type d’échantillon | Mammalian Cells |
Méthode PCR | 1-step RT-qPCR |
Matériau de départ | 10-100,000 Cells |
À utiliser avec (application) | Gene Expression |
Invitrogen™ Precision gRNA Synthesis Kit
Simple, ready-to-use, all-in-one expression vector system for CRISPR-Cas9. Transfection-ready guide RNA (gRNA) in as little as four hours.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The TaqMan Cells-to-CT Express Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
---|---|
Concept écologique | Less hazardous, less waste |
Méthode de détection | TaqMan |
Vitesse de réaction | 5 min. (Fast) |
Technologie d’isolement | Direct cell lysis |
Type d’échantillon | Mammalian Cells |
Cible de purification | RNA |
Méthode PCR | 2-step RT-qPCR |
Conditions d’expédition | Frozen, Dry Ice |
Type de kit | Direct Cell Lysis RT-qPCR |
À utiliser avec (application) | Gene Expression |
Forme | Liquid |
Contenu et stockage | Express Lysis Solution (room temperature or 4°C), Express ezDNase (-20°C), SuperScript IV VILO Master Mix (-20°C), SuperScript IV VILO No RT Control (-20°C), TaqMan Fast Advanced Master Mix (4°C) |
Compatibilité à haut débit | Yes |
Format | Kit |
Cible | cDNA |
Durée de conservation | 1 Year |
Matériau de départ | 10-100,000 Cells |
Marqueur ou colorant | ROX |
Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.