Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.

1 – 15 of 61 results

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

Pricing and Availability
Specifications

GC-Rich PCR Performance	High
Format	Tube
Product Type	1-Step qPCR kit
For Use With (Equipment)	7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Green Features	Fewer resources used and less waste, less hazardous
Starting Material	10-100,000 Cells
For Use With (Application)	Gene Expression
PCR Method	1-step RT-qPCR
Product Line	Cells-to-CT™, Power SYBR™ Green
Detection Method	SYBR

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

Pricing and Availability

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit

The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
GC-Rich PCR Performance	High
Fidelity (vs. Taq)	2X
Green Features	Fewer resources used and less waste, Less hazardous
Concentration	2X
For Use With (Application)	Gene Expression
High-throughput Compatibility	High-throughput Compatible
PCR Method	2-step RT-qPCR
Detection Method	SYBR
Format	Tube
Product Type	Fast Advanced Cells-to-Ct Kit
Sample Type	Mammalian Cells
Reaction Speed	7 min.
Reagent Type	Cell Lysis
Purification Target	Lysate
Starting Material	10-100,000 Cells
Product Line	Cells-to-CT™

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
GC-Rich PCR Performance	Medium
Includes Label or Dye	Yes
Overhang	Blunt
Concentration	2X
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR
Polymerase	Phire Hot Start II DNA Polymerase
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Tissue
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Starting Material	DNA

Applied Biosystems™ SequalPrep™ Normalization Plate Kit, 96-well

SequalPrep™ Normalization Plate Kit, 96-well

Pricing and Availability

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Pricing and Availability
Specifications

Shipping Condition	Wet or Dry Ice
Polymerase	Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance	Medium
Reaction Format	SuperMix or Master Mix
Product Type	Direct PCR Master Mix
Sample Type	Plant
Reaction Speed	Fast
Hot Start	Built-In Hot Start
Overhang	Blunt
Concentration	2X
Starting Material	DNA
For Use With (Application)	Genotyping,Hot-start PCR
PCR Method	Direct PCR

Invitrogen™ TaqMan™ MicroRNA Cells-to-C_T™ Kit

First to offer a simple, complete workflow for miRNA expression analysis from a few samples, or it can easily be incorporated into automated, high throughput applications.

Pricing and Availability

Invitrogen™ TaqMan™ Fast Cells-to-C_T™ Kit

The TaqMan™ Fast Cells-to-C_T™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

Pricing and Availability

Invitrogen™ Fast SYBR™ Green Cells-to-C_T™ Kit

Ambion™ Fast SYBR™ Green Cells-to-C_T™ Kit is a fast, easy, and robust method to go directly from cultured cells to real-time PCR results.

Pricing and Availability
Specifications

Shipping Condition	Dry Ice
Content And Storage	Store Stop Solution, DNase I and 20X RT Enzyme Mix at -20°C. Store Lysis Solution, 2X SYBR™ RT Buffer, Fast SYBR™ Green PCR Master Mix at 4°C. Store Nuclease-free Water at room temperature.
Purity or Quality Grade	UP (Ultra Pure)
Reverse Transcriptase	M-MLV
Purification Time	5 min.
Green Features	Fewer resources used and less waste, Less hazardous
Concentration	20X
For Use With (Application)	RT-PCR
Detection Method	SYBR
Polymerase	AmpliTaq Fast DNA Polymerase
Passive Reference Dye	ROX (Pre-mixed)
For Use With (Equipment)	7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Sample Type	Cells
Reaction Speed	Fast
Label or Dye	SYBR Green
Hot Start	Built-In Hot Start
Product Line	Cells-to-CT™, SYBR™

Invitrogen™ TaqMan™ Cells-to-C_T™ Control Kit

The Invitrogen™ TaqMan™ Cells-to-CT Control Kit is designed for use with the TaqMan™ Gene Expression Cells-to-CT Kit.

Pricing and Availability

MicroSEQ™ 500 16S rDNA PCR Kit

Pricing and Availability

Invitrogen™ SYBR™ Green Cells-to-C_T™ Control Kit

This kit is designed for use with the Power SYBRGreen and Fast SYBRGreen Cells-to-C_T™ Kits with sufficient reagents are provided for 100 reactions.

Pricing and Availability

Content And Storage	Store at -15°C to -25°C upon receipt and 2 to 8°C after initial use for up to 6 months.
Product Type	PCR Amplification Kit
Product Line	GlobalFiler™

Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit

The ChargeSwitch™-Pro PCR Clean-up Kit purifies high-quality DNA fragments without the use of ethanol or harsh salts known to interfere with enzymatic reactions.

Pricing and Availability
Specifications

Shipping Condition	Room Temperature
Target	DNA from Enzymatic Reactions (e.g. PCR)
Content And Storage	The ChargeSwitch™-Pro PCR Clean-up Kit contains columns, collection tubes, recovery tubes, binding buffer, wash buffer, and elution buffer. Store at room temperature. Guaranteed stable for 6 months when properly stored.
Format	Kit
Product Type	PCR Clean-Up Kit
Sample Type	PCR Products
Isolation Technology	ChargeSwitch™ Chemistry
Green Features	Fewer resources used and less waste, Less hazardous
For Use With (Application)	Next-Generation Sequencing (NGS),Cloning,Standard PCR
High-throughput Compatibility	Not High-throughput Compatible (Manual)
Product Line	ChargeSwitch™

Invitrogen™ RNA Control 250 with Manual

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

Pricing and Availability
Specifications

Content And Storage	RNA Control and THE RNA Storage Solution should be stored at –20°C
Format	Tube
Label or Dye	Unconjugated
Product Line	Ambion™

Direct PCR Reagents and Kits

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Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ MicroRNA Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ Fast SYBR™ Green Cells-to-CT™ Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ TaqMan™ Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ SYBR™ Green Cells-to-CT™ Control Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

Invitrogen™ ChargeSwitch™-Pro PCR Clean-Up Kit Greener ChoiceOffers one or more environmental benefits itemized in the U.S. FTC “Green Guides.”Learn More

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