Direct PCR Reagents and Kits

Direct PCR Reagents and Kits
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Thermo Scientific™ Phire Plant Direct PCR Master Mix
Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.
Polymérase | Phire Hot Start II DNA Polymerase |
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GC-Rich PCR Performance | Medium |
Vitesse de réaction | Fast |
Format de réaction | SuperMix or Master Mix |
Type de produit | Direct PCR Master Mix |
Hot Start | Built-In Hot Start |
Type d’échantillon | Plant |
En surplomb | Blunt |
Méthode PCR | Direct PCR |
Concentration | 2X |
Matériau de départ | DNA |
Conditions d’expédition | Wet or Dry Ice |
À utiliser avec (application) | Genotyping,Hot-start PCR |
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
Durée de fonctionnement | 10 min. |
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Format | Kit |
Technologie d’isolement | Silica Spin Column |
Type d’échantillon | PCR Products |
Conditions d’expédition | Room Temperature |
Thermo Scientific™ Phire Tissue Direct PCR Master Mix
Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.
GC-Rich PCR Performance | Medium |
---|---|
Vitesse de réaction | Fast |
Format de réaction | SuperMix or Master Mix |
Type de produit | Direct PCR Master Mix |
Type d’échantillon | Tissue |
En surplomb | Blunt |
Méthode PCR | Direct PCR |
Concentration | 2X |
Conditions d’expédition | Wet or Dry Ice |
À utiliser avec (application) | Genotyping,Hot-start PCR |
Comprend l’étiquette ou le colorant | Yes |
Polymérase | Phire Hot Start II DNA Polymerase |
Hot Start | Built-In Hot Start |
Matériau de départ | DNA |
Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer
These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.
Invitrogen™ TaqMan™ Cells-to-CT™ Express Lysis Reagents
This TaqMan Cells-to-CT Express Lysis Reagents product is a convenient, large volume offering of the cell lysis reagents used in our TaqMan Cells-to-CT Express Kit.
Invitrogen™ Single Cell Lysis Kit
Single-cell analysis has the potential to transform life science research. However, detecting and quantitating the minute amounts of RNA contained in single cells remains a significant challenge.
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Thermo Scientific™ GeneJET PCR Purification Kit
Thermo Scientific GeneJET PCR Purification Kit utilizes a proprietary silica-based membrane technology in the form of a convenient spin column, eliminating the need for tedious resin manipulations or toxic phenol-chloroform extractions.
Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
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Durée de fonctionnement | 5 min. |
Format | Spin Column |
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
Méthode de détection | Primer-probe |
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À utiliser avec (équipement) | 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Vitesse de réaction | Fast or Standard |
Gamme de produits | CellsDirect™, Platinum™, SuperScript™ |
Type d’échantillon | Cells |
Concentration | 2X |
À utiliser avec (application) | RT-PCR |
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
Température de réaction optimale | 42°C |
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Gamme de produits | Ambion™, Cells-to-cDNA™ |
Type d’échantillon | Mammalian Cells |
Type de produit final | cDNA |
Taille (produit final) | 7 kb or less |
Conditions d’expédition | Dry Ice |
Transcriptase inverse | M-MLV |
Temps de purification | 2 hr. |
À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
Contenu et stockage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
GC-Rich PCR Performance | High |
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Concept écologique | Fewer resources used and less waste, Less hazardous |
Méthode de détection | Primer-probe |
À utiliser avec (équipement) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
Vitesse de réaction | Standard |
Type de produit | Gene Expression Cells-to-C Kit |
Colorant de référence passive | ROX (Pre-mixed) |
Pureté ou qualité | UP (Ultra Pure) |
Type d’échantillon | Cell |
Méthode PCR | 2-step RT-qPCR |
Conditions d’expédition | Dry Ice |
Polymérase | DNA Polymerase |
Format | Tube |
Gamme de produits | Ambion™, Cells-to-CT™, TaqMan™ |
Transcriptase inverse | M-MLV |
Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.
Invitrogen™ RNA Control 250 with Manual
The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.
Format | Tube |
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Gamme de produits | Ambion™ |
Marqueur ou colorant | Unconjugated |
Contenu et stockage | RNA Control and THE RNA Storage Solution should be stored at –20°C |
ABsolute™ qPCR Master Mixes, SYBR Green-Based Detection
Thermo Scientific™ ABsolute qPCR Master Mixes offer sensitivity and reproducibility for real-time PCR applications.