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Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit
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Catalog No. 4399003
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100 reactions
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4399003 100 reactions
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Catalog No. 4399003 Supplier Invitrogen™ Supplier No. 4399003
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Description

Includes

Stop Solution: 500μL tube; DNase I: 55μL tube; 20X RT Enzyme Mix: 275μL tube; Lysis Solution: 5.5mL bottle; 2X RT Buffer: 5.5mL bottle; TaqMan Fast Universal PCR Master Mix: 4 x 1.25mL bottle

The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

  • Complete: Optimized workflow includes cell lysis reagents with gDNA removal, RT enzyme mix, RT buffer, and TaqMan™ Fast Universal PCR Master Mix
  • Fast: Prepare samples at room temperature in 7 minutes, including DNase treatment; TaqMan™ Fast Universal PCR Master Mix delivers real-time PCR results in ∼35 minutes
  • Convenient: Lyse samples in tube or directly in 96- or 384-well culture plates
  • Robust: Perform gene expression analysis on 10 to 100,000 cells per sample, with results equivalent to those from purified RNA
  • Efficient: Contains sufficient reagents to generate 500 real-time PCR results from 100 starting samples

Order Info

Shipping Condition: Dry ice

Specifications

Content And Storage

Contains:
• Stop Solution: 500 μL tube
• DNase I: 55 μL tube
• 20X RT Enzyme Mix: 275 μL tube
• Lysis Solution: 5.5 mL bottle
• 2X RT Buffer: 5.5 mL bottle
• TaqMan™ Fast Universal PCR Master Mix: 4 x 1.25 mL bottle

Store Stop Solution, DNase I, and 20X RT Enzyme Mix at -20°C.
Store Lysis Solution, 2X RT Buffer, and TaqMan™ Fast Universal PCR Master Mix at 4°C.
Detection Method Primer-probe
Format Tube
GC-Rich PCR Performance Low
PCR Method 2-step RT-qPCR
Polymerase DNA Polymerase
Reaction Speed Fast
For Use With (Equipment) 7500 Fast System, 7900HT Fast System, StepOne™, Fast Mode, StepOnePlus™, Fast Mode
Passive Reference Dye ROX (Pre-mixed)
Product Line Ambion, Cells-to-CT, TaqMan
Product Type Fast Cells-to-C Kit
Quantity 100 reactions
Reverse Transcriptase M-MLV
Shipping Condition Dry Ice
Sufficient For 100 Reactions
For Use With (Application) Gene Expression
No. of Reactions 100 reactions
Sample Type Cell
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For Research Use Only. Not for use in diagnostic procedures.