Direct PCR Reagents and Kits
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Résultats de la recherche filtrée
Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used and less waste, less hazardous |
| Méthode de détection | SYBR |
| Type de réactif | Cell Lysis |
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | 7 min. |
| Format | Tube |
| Type de produit | 1-Step qPCR kit |
| Gamme de produits | Cells-to-CT™, Power SYBR™ Green |
| Type d’échantillon | Mammalian Cells |
| Méthode PCR | 1-step RT-qPCR |
| Matériau de départ | 10-100,000 Cells |
| À utiliser avec (application) | Gene Expression |
Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit
The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.
Invitrogen™ SYBR™ Green Fast Advanced Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
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The SYBR Green Fast Advanced Cells-to-Ct Kit is a complete cell lysate system designed for gene expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used and less waste, Less hazardous |
| Méthode de détection | SYBR |
| Vitesse de réaction | 7 min. |
| Type de produit | Fast Advanced Cells-to-Ct Kit |
| Type d’échantillon | Mammalian Cells |
| Cible de purification | Lysate |
| Méthode PCR | 2-step RT-qPCR |
| Concentration | 2X |
| Conditions d’expédition | Dry Ice |
| À utiliser avec (application) | Gene Expression |
| Compatibilité à haut débit | High-throughput Compatible |
| Type de réactif | Cell Lysis |
| Fidélité (par rapport à Taq) | 2X |
| Format | Tube |
| Gamme de produits | Cells-to-CT |
| Matériau de départ | 10-100,000 Cells |
Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)
Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit with ROX, for ABI 7000, 7300, 7700, and 7900(HT) instruments
The CellsDirect One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 7000 System, 7300 System, 7700 System, 7900HT Fast System, 7900HT System, Eppendorf™ MasterCycler™ RealPlex, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Fast or Standard |
| Gamme de produits | CellsDirect, Platinum, SuperScript |
| Type d’échantillon | Cells |
| Concentration | 2X |
| À utiliser avec (application) | RT-PCR |
Invitrogen™ CellsDirect Resuspension & Lysis Buffers includes 10 ml Resuspension Buffer & 1 ml Lysis Buffer
These two buffers are to be used only as a complement to the SuperScript™ III Platinum™ CellsDirect Kits when large number of samples are processed.
Invitrogen™ CellsDirect™ One-Step qRT-PCR Kit
The CellsDirect™ One-Step qRT-PCR Kit delivers highly sensitive and specific real-time qPCR results directly from cells, without the need for an RNA purification step.
| Méthode de détection | Primer-probe |
|---|---|
| À utiliser avec (équipement) | 7500 Fast System, 7500 System, BioRad iQ5, BioRad MyiQ, Corbett RotorGene, MJ Opticon, Stratagene Mx3000P, Stratagene Mx3005P, Stratagene Mx4000, BioRad iCycler iQ, Cepheid SmartCycler, MJ Chromo4, QuantStudio 3 and 5 systems, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Fast or Standard |
| Gamme de produits | CellsDirect, Platinum, SuperScript |
| Type d’échantillon | Cells |
| Concentration | 2X |
| À utiliser avec (application) | RT-PCR |
Invitrogen™ Cells-to-cDNA™ II Cell Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-cDNA™ II Kit. Supplied in five 10 mL bottles.
Invitrogen™ Cells-to-cDNA™ II Kit
The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.
| Température de réaction optimale | 42°C |
|---|---|
| Gamme de produits | Ambion, Cells-to-cDNA |
| Type d’échantillon | Mammalian Cells |
| Type de produit final | cDNA |
| Taille (produit final) | 7 kb or less |
| Conditions d’expédition | Dry Ice |
| Transcriptase inverse | M-MLV |
| Temps de purification | 2 hr. |
| À utiliser avec (application) | Real Time PCR (qPCR),RT-PCR |
| Contenu et stockage | 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at -20°C. Nuclease-free water may be stored at any temperature. |
Invitrogen™ TaqMan™ Gene Expression Cells-to-CT™ Kit
Greener Choice
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
Offers one or more environmental benefits itemized in the U.S. FTC Green Guides.
Learn More
The TaqMan™ Gene Expression Cells-to-CT™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.
| GC-Rich PCR Performance | High |
|---|---|
| Concept écologique | Fewer resources used and less waste, Less hazardous |
| Méthode de détection | Primer-probe |
| À utiliser avec (équipement) | 7000 System, 7300 System, 7500 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system |
| Vitesse de réaction | Standard |
| Type de produit | Gene Expression Cells-to-C Kit |
| Colorant de référence passive | ROX (Pre-mixed) |
| Pureté ou qualité | UP (Ultra Pure) |
| Type d’échantillon | Cell |
| Méthode PCR | 2-step RT-qPCR |
| Conditions d’expédition | Dry Ice |
| Polymérase | DNA Polymerase |
| Format | Tube |
| Gamme de produits | Ambion, Cells-to-CT, TaqMan |
| Transcriptase inverse | M-MLV |
Invitrogen™ RNA Control 250 with Manual
The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.
| Format | Tube |
|---|---|
| Gamme de produits | Ambion |
| Marqueur ou colorant | Unconjugated |
| Contenu et stockage | RNA Control and THE RNA Storage Solution should be stored at –20°C |
Invitrogen™ Cells-to-Signal™ Lysis Buffer (10 ml Bottle)
The same lysis buffer found in the Invitrogen Cells-to-Signal™ Kit. Supplied in five 10 mL bottles.
Invitrogen™ PureLink™ PCR Purification Kit
The PureLink PCR Purification Kit provides a more convenient, rapid, and high-yielding purification process compared to other companies' kits.
| Durée de fonctionnement | 10 min. |
|---|---|
| Format | Kit |
| Technologie d’isolement | Silica Spin Column |
| Type d’échantillon | PCR Products |
| Conditions d’expédition | Room Temperature |
| Contenu et stockage | For better long-term performance, it is recommended to store the purification columns at 2°C to 8°C. |
Invitrogen™ PureLink™ Pro 96 PCR Purification Kit
The PureLink™ Pro 96 PCR Purification Kit combines advanced silica plate extraction chemistry with an optimized 96-well plate design for manual or automated processing of PCR cleanup in as little as 20 minutes.
Invitrogen™ PureLink™ PCR Micro Kit
PureLink PCR Micro Kits are ideal for purifying and concentrating DNA from low-yielding PCR or restriction digestion reactions.
| Volume d’élution max | 20 μL |
|---|---|
| Technologie d’isolement | Silica Spin Column |
| Type de produit | PCR Micro Kit |
| Conditions d’expédition | Room Temperature |
| À utiliser avec (application) | Cloning,Standard PCR |
| Contenu et stockage | Kit includes columns, binding and wash buffers, recovery tubes, and the manual. Store at room temperature. |
| Volume de matériau de démarrage | 125 μL |
| Compatibilité à haut débit | Not High-throughput Compatible (Manual) |
| Volume d’élution min | 5 μL |
| Durée de fonctionnement | 10 min. |
| Format | Spin Column |
| Cible | DNA from Enzymatic Reactions (e.g. PCR) |
| Plage de séparation | 100 bp to 12 kb |
| Gamme de produits | PureLink |