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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1 – 15 of 58 results
1

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Green Features Fewer resources used and less waste, less hazardous
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, Power SYBR™ Green
Detection Method SYBR
2

Applied Biosystems™ CLA IdentiFiler™ Direct PCR Amplification Kit

The CLA (Cell Line Authentication) IdentiFiler Direct PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

3

Thermo Scientific™ Phire Animal Tissue Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Animal Tissue Direct PCR Kit has been developed for amplification of DNA directly from a wide variety of animal tissues including mice, fish, birds and insects.

4

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
5

Thermo Scientific™ Phire Tissue Direct PCR Master Mix

Thermo Scientific Phire Tissue Direct PCR Master Mix has been developed for amplification of DNA directly from a wide variety of tissues obtained from mice, human, fish, birds and insects.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance Medium
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
Polymerase Phire Hot Start II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Tissue
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
6

Thermo Scientific™ Phusion Blood Direct PCR Kit

Thermo Scientific Phusion Blood Direct PCR Kit is developed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Shipping Condition Wet or Dry Ice
Polymerase Phusion Blood II DNA Polymerase
GC-Rich PCR Performance High
Reaction Format Separate Components
Product Type Direct PCR Kit
Sample Type Blood
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2 U/μL
Starting Material DNA
PCR Method Direct PCR
7

Thermo Scientific™ Phire Plant Direct PCR Kit (without sampling tools)

Thermo Scientific Phire Plant Direct PCR Kit is designed to amplify DNA directly from plant samples. No DNA purification is required prior to PCR.

8

Thermo Scientific™ Phusion Human Specimen Direct PCR Kit

Thermo Scientific Phusion Human Specimen Direct PCR Kit delivers unprecedented convenience for DNA amplification by allowing PCR directly from unpurified human samples.

9

Thermo Scientific™ Phusion Blood Direct PCR Master Mix

Thermo Scientific Phusion Blood Direct PCR Master Mix is designed for amplification of DNA from whole blood. It eliminates the need for a separate DNA purification step prior to PCR.

Shipping Condition Wet or Dry Ice
GC-Rich PCR Performance High
Includes Label or Dye Yes
Overhang Blunt
Concentration 2X
For Use With (Application) Genotyping
PCR Method Direct PCR
Polymerase Phusion Blood II DNA Polymerase
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Blood
Reaction Speed Fast
Hot Start Built-In Hot Start
Starting Material DNA
10

Thermo Scientific™ Mutation Generation System Kit

Perform functional analysis of proteins with these transposon-based tools.

11

Thermo Scientific™ Template Generation System II Kit

Perform transposon-mediated insertion of primer binding sites into target DNA for sequencing or PCR amplification.

12

Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.

13

Thermo Scientific™ MuA Transposase

Transposon-based tools for functional analysis of proteins.

14

Invitrogen™ Cells-to-cDNA™ II Kit  DFS Item

The Ambion™ Cells-to-cDNA™ II Kit (patent pending) produces cDNA from cultured mammalian cells in less than 2 hr., no RNA isolation is required.

Shipping Condition Dry Ice
Content And Storage 1X PBS, Cell Lysis II Buffer, μL DNase 1, 10X RT Buffer, M-mLV Reverse Transcriptase, RNase Inhibitor, dNTP Mix, Random Decamers, Oligo(dT)18 Primers, Armored RNA™ Control, Armored RNA™ Primer Pair, and Endogenous Primer Pair should be stored at
-20°C. Nuclease-free water may be stored at any temperature.
Reverse Transcriptase M-MLV
Size (Final Product) 7 kb or less
Final Product Type cDNA
Sample Type Mammalian Cells
Purification Time 2 hr.
Optimal Reaction Temperature 42°C
For Use With (Application) Real Time PCR (qPCR),RT-PCR
Product Line Ambion, Cells-to-cDNA
15

Invitrogen™ RNA Control 250 with Manual  DFS Item

The Invitrogen RNA Control 250 provides a precise, accurate, and reproducible RNA concentration reference for use in conjunction with NanoDrop™ Spectrophotometers.

Content And Storage RNA Control and THE RNA Storage Solution should be stored at –20°C
Format Tube
Label or Dye Unconjugated
Product Line Ambion