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Direct PCR Reagents and Kits

PCR products that allow for the amplification of DNA directly from unpurified source material. Includes various kits, master mixes, enzymes, and reagents.
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1

Thermo Scientific™ Phire Plant Direct PCR Master Mix

Thermo Scientific Phire Plant Direct PCR Master Mix is designed to amplify DNA directly from plant samples.

Shipping Condition Wet or Dry Ice
Polymerase Phire Hot Start II DNA Polymerase
GC-Rich PCR Performance Medium
Reaction Format SuperMix or Master Mix
Product Type Direct PCR Master Mix
Sample Type Plant
Reaction Speed Fast
Hot Start Built-In Hot Start
Overhang Blunt
Concentration 2X
Starting Material DNA
For Use With (Application) Genotyping,Hot-start PCR
PCR Method Direct PCR
2

Invitrogen™ TaqMan™ Fast Cells-to-CT™ Kit  DFS Item
GREENER_CHOICE

The TaqMan™ Fast Cells-to-CT™ Kit makes it possible to use Fast-cycling, real-time PCR chemistry and instrumentation to perform gene expression analysis directly from cultured cells without RNA purification.

4

Invitrogen™ Cells-to-CT Bulk RT Reagents  DFS Item
GREENER_CHOICE

Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription in the TaqMan™ Gene Expression Cells-to-CT™ Kit (20X RT enzyme mix and 20X RT buffer).

5
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Invitrogen™ PureLink™ PCR Purification Binding Buffer B2  DFS Item

PureLink™ PCR Purification Binding Buffer B2 is the binding buffer used with the PureLink™ PCR Purification Kit.

6

Applied Biosystems™ CLA GlobalFiler™ PCR Amplification Kit

The CLA (Cell Line Authentication) GlobalFiler PCR Amplification Kit simplifies the amplification step during authentication of cell lines.

7

Invitrogen™ Cells-to-CT™ 1-Step TaqMan™ Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step TaqMan™ Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT, TaqMan
Detection Method TaqMan
8

Invitrogen™ Cells-to-CT™ Bulk Fast Advanced RT Reagents  DFS Item
GREENER_CHOICE

Invitrogen Fast Advanced Cells-to-CT Bulk RT Reagents are separately-available, large-quantity versions of the reagents used for reverse transcription (RT) in the TaqMan Fast Advanced Cells-to-CT Kit and the SYBR Green Fast Advanced Cells-to-CT Kit.

9

Applied Biosystems™ MicroSEQ™ D2 rDNA Fungal PCR Kit
GREENER_CHOICE

This kit is the PCR component of the MicroSEQ™ Fungal Identification System, which provides an easy-to-use DNA sequence-based method to identify most fungi.

10

Invitrogen™ Cells-to-CT™ 1-Step Power SYBR™ Green Kit  DFS Item
GREENER_CHOICE

Like other members of the Cells-to-CT™ product family, the Cells-to-CT™ 1-Step Power SYBR™ Green Kit makes it possible to perform expression analysis directly from cultured cells without RNA purification.

GC-Rich PCR Performance High
Format Tube
Product Type 1-Step qPCR kit
For Use With (Equipment) 7500 Fast System, 7500 System, 7900HT Fast System, 7900HT System, QuantStudio™, ViiA™ 7 System, StepOnePlus™ System, StepOne™ System, Real-Time PCR Systems or Thermal Cyclers, 7500 Fast Dx System, QuantStudio 6 and 7 Pro systems, QuantStudio 7 Flex system, QuantStudio 3 and 5 systems, QuantStudio 12k Flex system, QuantStudio 6 Flex system
Sample Type Mammalian Cells
Reaction Speed 7 min.
Reagent Type Cell Lysis
Starting Material 10-100,000 Cells
For Use With (Application) Gene Expression
PCR Method 1-step RT-qPCR
Product Line Cells-to-CT™, Power SYBR™ Green
Detection Method SYBR
11

Thermo Scientific™ Mutation Generation System Kit

Perform functional analysis of proteins with these transposon-based tools.

12

Thermo Scientific™ Template Generation System II Kit

Perform transposon-mediated insertion of primer binding sites into target DNA for sequencing or PCR amplification.

13

Thermo Scientific™ MuA Transposase (concentrated)

Perform functional analysis of proteins with these transposon-based tools.

14

Applied Biosystems™ QuantStudio™ 12K Flex OpenArray™ Calibration Kit  DFS Item

Offered in order to maintain the QuantStudio™ 12K Flex Real Time PCR System OpenArray™ block type.