Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™
CAS: 10043-35-3 Synonym: TBE
HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™
Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol
PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™
Commonly used buffer
Gibco™ PBS, pH 7.4
Gibco PBS, pH 7.4, is a balanced salt solution ideal for cell culture applications. It lacks calcium, magnesium, and phenol red, preventing cell adhesion and assay interference. Trusted for quality, Gibco PBS ensures reliable results in cell washing, transport, and reagent preparation.
Gibco™ DPBS, no calcium, no magnesium
Dulbecco's Phosphate-Buffered Saline (DPBS) is a balanced salt solution used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue, diluting cells for counting, and preparing reagents.
Thermo Scientific Chemicals TRIS, 1.0M buffer soln., pH 8.0, 0.2 micron filtered
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Thermo Scientific™ TRIS, 1.0M buffer soln., pH 8.0
CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O
Tris-Glycine, 10X Solution (Electrophoresis), Fisher BioReagents™
Tris Hydrochloride (Small White Flakes/Molecular Biology), Fisher BioReagents
CAS: 1185-53-1 Molecular Formula: C4H12ClNO3 Molecular Weight (g/mol): 157.594 InChI Key: QKNYBSVHEMOAJP-UHFFFAOYSA-N Synonym: Tris(hydroxymethyl)aminomethane Hydrochloride PubChem CID: 93573 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydrochloride SMILES: C(C(CO)(CO)N)O.Cl
PBS Tablets, Phosphate Buffered Saline, Fisher BioReagents™
Phosphate Buffered Saline Tablets, CAS Number-7647-14-5, 7447-40-7, 100 tablets, Clear, Gray, White, 5/8 in., 600 SEC max., CAUTION!