Buffers and Standards

Hydrogen ion buffers comprising a mixture of a weak acid and its conjugate base or vice-versa and used to stabilize pH; also diluents, washing solutions, and standard-value solutions used for a broad array of scientific calibration purposes.

The buffers used to calibrate pH meters may be certified and/or traceable to the National Institute of Standards and Technology (NIST). These buffers may also be color-coded for easy identification:

Red: pH 4.0
Yellow: pH 7.0
Blue: pH 10.0

Chromatography Standards (6,439)

General Chemistry Solutions (2,860)

Spectroscopy Standards (2,009)

Matrix Blanks and Standardization Solutions (1,205)

Electrochemistry Buffers (988)

Life Science Buffers (945)

Electrochemistry Standards (647)

Environmental Testing Standards (401)

Karl Fischer Buffers and Reagents (186)

Kjeldahl Buffers and Reagents (57)

Viscosity Standards (42)

Quality Control and Proficiency Samples (16)

Isotope Standards (4)

Matrix Modifiers (3)

Osmolality Standards (3)

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1 – 15 of 11,800 results

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TE Buffer, Tris-EDTA, 1X Solution, pH 8.0, Molecular Biology, Fisher BioReagents

Pricing and Availability
Specifications

Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris-EDTA
Grade	Molecular Biology
DNase	DNase free
Filtered Through	Filtered through a 5-micron filter.
ChemAlert Storage Symbol	Gray
Name Note	1X Solution, pH 8.0
CAS	7732-18-5
Protease	Protease free
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. Avoid contact with skin and eyes. Do not breathe dust. Do not breathe vapors or spray mist. Wash off immediately with soap and plenty of water removing all contaminated clothes and shoes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Remove from exposure, lie down. Move to fresh air. If breathing is difficult, give oxygen. If not breathing, give artificial respiration. If not breathing, give artificial respiration. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
Health Hazard 2	CAUTION!
pH	7.4 to 8.1
Synonym	TE
Recommended Storage	RT

Phosphate Buffered Saline (PBS), 10X Powder, pH 7.4, Fisher BioReagents™

Phosphate Buffered Saline (PBS), 10X Powder provides a convenient way to make 1 L of 10X stock PBS solution.

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Tris (Tromethamine) (Granular/USP), Fisher Chemical™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 MDL Number: MFCD00004679 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trishydroxymethylaminomethane,Tris Buffer,Tris,THAM,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
MDL Number	MFCD00004679
SMILES	C(C(CO)(CO)N)O
Synonym	Trishydroxymethylaminomethane,Tris Buffer,Tris,THAM,Trizma™,2-Amino-2-(hydroxymethyl)-1,3-propanediol,Tris base,Tris(hydroxymethyl)aminomethane,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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MES (Fine White Crystals), Fisher BioReagents

CAS: 145224-94-8 Molecular Formula: C6H15NO5S Molecular Weight (g/mol): 213.248 InChI Key: MIIIXQJBDGSIKL-UHFFFAOYSA-N Synonym: 2-(4-Morpholino)ethane Sulfonic Acid,2-(N-Morpholino)ethanesulfonic acid hydrate PubChem CID: 16218417 IUPAC Name: 2-morpholin-4-ylethanesulfonic acid;hydrate SMILES: C1COCCN1CCS(=O)(=O)O.O

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Specifications

PubChem CID	16218417
CAS	145224-94-8
Molecular Weight (g/mol)	213.248
SMILES	C1COCCN1CCS(=O)(=O)O.O
Synonym	2-(4-Morpholino)ethane Sulfonic Acid,2-(N-Morpholino)ethanesulfonic acid hydrate
IUPAC Name	2-morpholin-4-ylethanesulfonic acid;hydrate
InChI Key	MIIIXQJBDGSIKL-UHFFFAOYSA-N
Molecular Formula	C6H15NO5S

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Tris Base (White Crystals or Crystalline Powder/Triple-Crystallized), Fisher BioReagents™

For sensitive molecular biology, tissue culture, and electrophoresis methods | CAS: 77-86-1 | C4H11NO3 | 121.136 g/mol

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Tris Base (White Crystals or Crystalline Powder/Molecular Biology), Fisher BioReagents™

CAS: 77-86-1 Molecular Formula: C4H11NO3 Molecular Weight (g/mol): 121.136 InChI Key: LENZDBCJOHFCAS-UHFFFAOYSA-N Synonym: Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol PubChem CID: 6503 ChEBI: CHEBI:9754 IUPAC Name: 2-amino-2-(hydroxymethyl)propane-1,3-diol SMILES: C(C(CO)(CO)N)O

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Specifications

PubChem CID	6503
CAS	77-86-1
Molecular Weight (g/mol)	121.136
ChEBI	CHEBI:9754
SMILES	C(C(CO)(CO)N)O
Synonym	Trimethylol Aminomethane,Tris(hydroxymethyl)aminomethane,2-Amino-2-(hydroxymethyl)-1,3-propanediol,THAM,Tris base,Trometamol
IUPAC Name	2-amino-2-(hydroxymethyl)propane-1,3-diol
InChI Key	LENZDBCJOHFCAS-UHFFFAOYSA-N
Molecular Formula	C4H11NO3

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TBE Buffer, Tris-Borate-EDTA, 10X Solution, Electrophoresis, Fisher BioReagents™

CAS: 10043-35-3 Synonym: TBE

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Specifications

CAS	10043-35-3
Synonym	TBE

TBS, Tris Buffered Saline, 10X Solution, pH 7.4, Molecular Biology, Fisher BioReagents™

Pricing and Availability
Specifications

Boiling Point	100°C
Color	Colorless
Physical Form	Liquid
Chemical Name or Material	Tris Buffered Saline
Grade	Molecular Biology
DNase	DNase free
ChemAlert Storage Symbol	Gray
Name Note	10X Solution
Concentration or Composition (by Analyte or Components)	10X solution contains 1.37M Sodium Chloride, 0.027M Potassium Chloride, and 0.25M Tris/Tris-HCl.
CAS	7732-18-5
Protease	Protease free
pH	7.5
Synonym	TBS
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT

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HEPES (Fine White Crystals/Molecular Biology), Fisher BioReagents™

Commonly used buffering agent | CAS: 7365-45-9 | C8H18N2O4S | 238.30 g/mol

Pricing and Availability
Specifications

Molecular Weight (g/mol)	238.30
ChEBI	CHEBI:42334
Color	White
Chemical Name or Material	HEPES
Grade	Molecular Biology
SMILES	OCCN1CCN(CCS(O)(=O)=O)CC1
Identification	Pass Test
DNase	DNase free
Merck Index	15, 4689
InChI Key	JKMHFZQWWAIEOD-UHFFFAOYSA-N
ChemAlert Storage Symbol	Gray
Assay Percent Range	≥99 %
PubChem CID	23831
Absorbance	0.01 max. (0.1M solution) at 280nm
Percent Purity	≥99%
CAS	7365-45-9
Protease	Protease free
Health Hazard 3	Emergency Overview Causes eye, skin, and respiratory tract irritation. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Obtain medical attention. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Obtain medical attention. Do not induce vomiting. Obtain medical attention. Obtain medical attention. . NFPA Health:2 Flammability:1 Instability:1
MDL Number	MFCD00006158
Health Hazard 2	WARNING!
Solubility Information	Soluble in water
pH	5.0 to 6.5
Purity Grade Notes	DNase-, RNase- and Protease-Free
Recommended Storage	RT
Molecular Formula	C8H18N2O4S

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TAE Buffer, Tris-Acetate-EDTA, 50X Solution, Electrophoresis, Fisher BioReagents™

Tris-Acetate-EDTA, CAS Number-77-86-1, 60-00-4, 6850-28-8, TAE, 4L, Gray, Tris (24%), Acetic Acid (5.0%), and EDTA (<2%)., DNase free, Pass Test, Filtered through a 0.2-micron filter., Electrophoresis, 50X Solution, Poly CUBE, Liquid, Protease free, DNase-, RNase- and Protease-Free, RT

Pricing and Availability
Specifications

Name Note	50X Solution
Concentration or Composition (by Analyte or Components)	Tris (24%), Acetic Acid (5.0%), and EDTA (<2%).
CAS	6850-28-8
Protease	Protease free
Physical Form	Liquid
Grade	Electrophoresis
Synonym	TAE
Purity Grade Notes	DNase-, RNase- and Protease-Free
DNase	DNase free
Recommended Storage	RT
Filtered Through	Filtered through a 0.2-micron filter.
ChemAlert Storage Symbol	Gray

PBS, Phosphate Buffered Saline, 10X Solution, Fisher BioReagents™

Commonly used buffer

Pricing and Availability
Specifications

Physical Form	Liquid
Recommended Storage	Room temperature

Buffer Solution, pH 4.00, Color-Coded Red (Certified), Fisher Chemical™

pH/temperature conversions given on label

Pricing and Availability
Specifications

Boiling Point	100°C
Description	pH/temperature conversions given on label.
Color	Red
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 4.00, Color-Coded Red
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Density	1.0g/cm³
Absorbance	Pass Test
Concentration or Composition (by Analyte or Components)	Potassium Hydrogen Phthalate
CAS	16423-68-0
Health Hazard 3	Emergency Overview Contains a known or suspected carcinogen. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
Solubility Information	Soluble in water
pH	4.0
Specific Gravity	1
Melting Point	0°C

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Buffer Solution, pH 3.00 (Certified), Fisher Chemical™

Pricing and Availability
Specifications

Boiling Point	100°C
Color	Undesignated
Traceability to NIST	Traceable to NIST.
Physical Form	Liquid
Chemical Name or Material	Buffer Solution, pH 3.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Density	0.7g/cm³
CAS	7732-18-5
Health Hazard 3	Emergency Overview May cause eye, skin, and respiratory tract irritation. The toxicological properties have not been fully investigated. Use personal protective equipment. Ensure adequate ventilation. Wash off immediately with plenty of water for at least 15 minutes. Get medical attention immediately if symptoms occur. Rinse immediately with plenty of water, also under the eyelids, for at least 15 minutes. Obtain medical attention. Move to fresh air. If breathing is difficult, give oxygen. Get medical attention immediately if symptoms occur. Do not induce vomiting. Do not induce vomiting. Obtain medical attention. NFPA Health:1 Flammability:0 Instability:0
MDL Number	MFCD00146206
Health Hazard 2	CAUTION!
pH	3.00
Melting Point	0°C

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Buffer Solution, pH 5.00 (Certified), Fisher Chemical™

Pricing and Availability
Specifications

Boiling Point	100°C
CAS	7732-18-5
MDL Number	MFCD00146206
Traceability to NIST	Traceable to NIST.
Packaging	Poly Bottle
Physical Form	Liquid
pH	5.0
Chemical Name or Material	Buffer Solution, pH 5.00
Grade	Certified
Identification	Pass Test
ChemAlert Storage Symbol	Gray
Melting Point	0°C

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Buffer Concentrate, pH 7.00 (Certified), Fisher Chemical™

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Specifications

CAS	7664-38-2
Molecular Weight (g/mol)	380.17
MDL Number	MFCD00146206
Traceability to NIST	Traceable to NIST.
Packaging	Poly Bottle
Physical Form	Liquid
Chemical Name or Material	Buffer Concentrate, pH 7.00
Grade	Certified
Identification	Pass Test
InChI Key	UEUXEKPTXMALOB-UHFFFAOYSA-J
Molecular Formula	C10H12N2Na4O8
ChemAlert Storage Symbol	Gray

Buffers and Standards

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