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Thermo Scientific™ DH5α Competent Cells

Numéro de catalogue. FEREC0111
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20 Reactions
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FEREC0111 20 Reactions
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Numéro de catalogue. FEREC0111 Fournisseur Thermo Scientific™ Code fournisseur EC0111
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Description

Chemically competent E. coli cells recommended for routine subcloning into plasmid vectors. Subcloning efficiency cells are not suitable for the generation of cDNA libraries.

  • The φ80dlacZΔM15 marker provides α-complementation of the β-galactosidase gene from pUC or similar vectors to allow blue/white colony screening on bacterial agar plates containing Bluo-Gal or X-Gall
  • Transformation efficiency: >1x106 cfu/μg pUC19 DNA
  • Suitable for routine cloning applicationsConvenient economical packaging
  • Contain genetic markers that allow for blue/white colony screening
  • DH5α Competent Cells for Subcloning are suitable for the standard DNA cloning applications:
    • DNA cloning following PCR or restriction digestion reactions
    • Subcloning (transferring DNA from a parent vector to a destination vector)
    • Plasmid isolation
  • Genotype: F– φ80lacZΔ M15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK– mK+) phoA supE44 λ- thi–1 gyrA96 relA1
  • For Research Use Only. Not for use in diagnostic procedures.

Spécifications

Description Chemically Competent
Contains F' Episome Lacks F' Episome
Content And Storage Dry Ice (-80°C)
Bacterial or Yeast Strain DH5α
Species E. coli
Format Tube
For Use With (Application) Cloning
Quantity 20 Reactions