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Gibco™ Freestyle™ CHO Expression Medium DFS Item

Animal origin-free, chemically defined, protein-free medium

$145.95 - $613.20

Specifications

Additive No glutamine, no phenol red
Cell Line CHO, FreeStyle™ CHO-S Cells
Format Liquid
Storage Requirements Storage conditions: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture
View More Specs

 Disclaimers

For Research Use Only. Not for use in diagnostic procedures.

Products
Catalog Number Mfr. No. Quantity Price Quantity    

12651014

DFS Item
 
life technologies
12651014
1000mL Each for $145.95

12651022

DFS Item
 
life technologies
12651022
6 x 1000mL Each for $613.20
Description & Specifications

Specifications

Additive No glutamine, no phenol red
Cell Line CHO, FreeStyle™ CHO-S Cells
Format Liquid
Storage Requirements Storage conditions: 2°C to 8°C. Protect from light. Shelf life: 12 months from date of manufacture

FreeStyle CHO Expression Medium is specifically developed to support the growth and transient transfection of CHO-S cells in suspension without adaptation. This medium requires supplementation with 8 mM glutamine before use.

  • Formulation without hypoxanthine and thymidine for use with or without dhfr systems
  • Ability to support high-density suspension growth and transient transfection of CHO-S cells
  • Animal origin-free, protein-free and chemically defined formulation
  • Easy use as part of the FreeStyle MAX CHO Expression System for protein production
  • Scalability in spinner flasks and bioreactors

Formulation without hypoxanthine and thymidine for use with or without dhfr systems

  • Gibco FreeStyle CHO Expression Medium is made without hypoxanthine and thymidine for use in dihydrofolate reductase (dhfr) amplification systems
  • Non-dhfr systems require the addition of HT Supplement before use
Ability to support high-density suspension growth and transient transfection of CHO-S cells
  • Gibco FreeStyle CHO cells in suspension can be cultured directly into Gibco FreeStyle CHO Expression Medium without adaptation
  • Cells adapted to other commercially available serum-free media can be subcultured directly into Gibco FreeStyle CHO Expression Medium, usually without any further adaptation
  • Cells usually require adaptation from serum-containing formulations
  • Cells cultured in Gibco FreeStyle CHO Expression Medium can be transfected using lipid-based transfection reagents, such as FreeStyle MAX reagent
Animal origin-free, protein-free and chemically defined formulation
  • Gibco FreeStyle CHO Expression Medium is animal origin–free, protein-free, and chemically defined, allowing for easier purification of your protein of interest
  • Gibco chemically defined media contain no proteins, hydrolysates, or components of unknown composition
Scalability in spinner flasks and bioreactors
  • Protein production using Gibco FreeStyle CHO Expression Medium can be scaled up in spinner flasks or bioreactors
  • The appropriate spinner or impeller speed and seeding density should be optimized for each system
  • Depending on the impeller design and speed, it may be necessary to supplement Gibco FreeStyle CHO Expression Medium with additional Pluronic™ F-68 to avoid sheer stress in the culture

Antibody and Cell Culture Production, Bioproduction, Cell Culture, Mammalian Cell Culture

Shipping Condition: Room temperature

Gibco FreeStyle CHO Expression Medium is manufactured at a cGMP-compliant facility located in Grand Island, New York. The facility is registered with the FDA as a medical device manufacturer and is certified to ISO 13485 standards.