Invitrogen™ ElectroMAX™ DH10B T1 Phage-Resistant Competent Cells

ElectroMAX DH10B T1 Phage-Resistant Competent Cells offer transformation efficiencies that are among the highest we offer and can exceed 1 x 10¹⁰ cfu/μg plasmid DNA in efficiency. They are ideal for applications requiring high-efficiency transformation, including cDNA and gDNA library construction.

ElectroMAX DH10B cells:
• Maximize yield of transformants from limited cloning products
• Permit efficient cloning of methylated DNA
• Are resistant to T1 and T5 bacteriophages
• Support blue/white screening by α-complementation on plates containing X-Gal or Bluo-Gal
• Deliver high-yield plasmid preparations for downstream applications

Phage-resistant representative libraries from a single transformation
The tonA mutation in DH10B cells confers resistance to bacteriophages T1 and T5, safeguarding valuable libraries against contamination. Additionally, mutations in the methylation-dependent restriction system (mcrA, mcrBC, and mrr) make ElectroMAX DH10B T1 Phage-Resistant Cells ideal for construction of genomic libraries of both prokaryotic and eukaryotic genomic DNA and allow for efficient plasmid rescue from eukaryotic genomes. ElectroMAX DH10B T1 Phage-Resistant Cells are suitable for construction of gene banks, for the generation of cDNA libraries using plasmid-derived vectors, and for situations when DNA is limiting. This strain also has the Φ80lacZΔM15 genotype, providing for the option of blue/white screening on plates containing either X-Gal or Bluo-Gal. Finally, the endA1 mutation makes DH10B an excellent vehicle for amplifying plasmid DNA for subsequent extraction and purification.

Genotype
F^-mcrA Δ(mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 endA1 araD139Δ(ara, leu)7697 galU galK λ^-rpsL nupG tonA

Find the strain and format that fits your needs
• DH10B cells are available in both electrocompetent and chemically competent formats.
• ElectroMAX DH10B cells (Cat. No.18290015) are suitable for cDNA or gDNA library construction but are not resistant to T1 and T5 phage infection.
• MegaX DH10B T1^R Electrocomp Cells (Cat. No. C640003) have the highest transformation efficiency (>3 x 10¹⁰ cfu/μg plasmid DNA) and also offer the benefit of T1 and T5 phage resistance.