Chemically modified bacterial cells capable of uptaking DNA from the environment via transformation. Competent cultures of E. coli are used in the lab for various procedures including cloning, protein expression, and genetic library creation.
High efficiency, chemically competent E. coli cells. The DH10B strain is suitable for cloning DNA containing methylcytosine, 5-hydroxymethylcytosine, and methyladenine, allowing both prokaryotic and eukaryotic genomic DNA to be cloned efficiently.
Suitable for the expression of non-toxic heterologous genes. The strain contains the lambda DE3 prophage that carries the gene for T7 RNA polymerase under control of a lacUV5 promoter, allowing expression of the T7 RNA polymerase to be induced with IPTG.
MultiShot FlexPlate Stbl3 Chemically Competent E. coli cells are cloning competent cells that are pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
MultiShot TOP10 Chemically Competent E. coli Cells provide high-efficiency transformation for routine cloning in an automation-ready 96-well format. Ideal for stable propagation of high-copy plasmids and blue/white screening in high-throughput workflows.
One Shot MAX Efficiency DH5α-T1R Competent Cells are derived from the popular DH5α strain and carry the tonA (fhuA) mutation that confers resistance to T1 and T5 phage.
MultiShot FlexPlate DH10B T1R chemically competent E. coli cells are cloning competent cells pre-aliquoted in a 96-well PCR plate to increase transformation productivity.
BL21 host strain that expresses T7 RNA polymerase and also encode T7 lysozyme that suppresses basal expression of toxic target proteins prior to induction.
MAX Efficiency™ DH5α Competent Cells are a well-known, versatile strain that is used in many everyday cloning applications. Prepared by a proprietary modification of the Hanahan method, these cells are suitable for high efficiency transformations in applications where low amount of DNA is used or to construct libraries using plasmid-derived vectors.
MultiShot StripWell Mach1 T1R chemically competent E. coli cells are exceptionally fast-growing cloning competent cells packaged in a rack containing 12 strips of 8 tubes for increased productivity of bacterial transformations.
MultiShot Stbl3 Chemically Competent E. coli Cells support cloning of unstable vectors with direct repeats. Automation-compatible 96-well format ensures high-throughput use while the Stbl3 strain minimizes recombination and maintains complex constructs.
One Shot PIR2 Chemically Competent E. coli cells are for use with vectors that contain the R6Kγ origin of replication (e.g., pUni/V5-His-TOPO for recombination with an Echo cloning system).